Ramalin inhibits LPS‐induced autophagic response in macrophages through the downregulation of iNOS expression
Activation of macrophages is essential for the host defense system and accumulating evidence suggests that autophagy is an important component in macrophage activity. Prolonged activation of macrophages, however, becomes detrimental and contributes to the progression of chronic inflammatory diseases...
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Online Access: | http://dx.doi.org/10.1096/fasebj.27.1_supplement.888.2 |
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crwiley:10.1096/fasebj.27.1_supplement.888.2 2024-06-02T07:58:14+00:00 Ramalin inhibits LPS‐induced autophagic response in macrophages through the downregulation of iNOS expression Park, Hye‐Jin Yim, Joung Han Lee, Hong Kum Pyo, Suhkneung 2013 http://dx.doi.org/10.1096/fasebj.27.1_supplement.888.2 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 27, issue S1 ISSN 0892-6638 1530-6860 journal-article 2013 crwiley https://doi.org/10.1096/fasebj.27.1_supplement.888.2 2024-05-03T10:52:47Z Activation of macrophages is essential for the host defense system and accumulating evidence suggests that autophagy is an important component in macrophage activity. Prolonged activation of macrophages, however, becomes detrimental and contributes to the progression of chronic inflammatory diseases with abnormal cell death. Therefore, controlling the overactivation of macrophages may be a new therapeutic strategy for various inflammatory diseases. Here we showed that ramalin, an antioxidant compound from the Antarctic lichen Ramalina terebrata , inhibited both LPS‐induced autophagy and iNOS in macrophages. To verify the role of NO in autophagy, we compared autophagy‐related gene expression in LPS‐stimulated and SNP‐stimulated macrophages by western blotting. Interestingly, ramalin did not suppress NO and autophagy in macrophages exposed to NO donor, SNP. In addition, inhibition of NO production by SMT significantly reduced LPS‐induced autophagy. These finding imply that ramalin might suppress LPS‐induced autophagy by inhibiting NO secretion. Ramalin also showed inhibitory effect on LPS‐induced NF‐κB and MAPK activation. Overall, these finding indicate that ramalin suppresses autophagy in LPS‐stimulated macrophages by preventing NO production and downregulating both NF‐κB and MAPK activation. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The Antarctic The FASEB Journal 27 S1 |
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Wiley Online Library |
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crwiley |
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English |
description |
Activation of macrophages is essential for the host defense system and accumulating evidence suggests that autophagy is an important component in macrophage activity. Prolonged activation of macrophages, however, becomes detrimental and contributes to the progression of chronic inflammatory diseases with abnormal cell death. Therefore, controlling the overactivation of macrophages may be a new therapeutic strategy for various inflammatory diseases. Here we showed that ramalin, an antioxidant compound from the Antarctic lichen Ramalina terebrata , inhibited both LPS‐induced autophagy and iNOS in macrophages. To verify the role of NO in autophagy, we compared autophagy‐related gene expression in LPS‐stimulated and SNP‐stimulated macrophages by western blotting. Interestingly, ramalin did not suppress NO and autophagy in macrophages exposed to NO donor, SNP. In addition, inhibition of NO production by SMT significantly reduced LPS‐induced autophagy. These finding imply that ramalin might suppress LPS‐induced autophagy by inhibiting NO secretion. Ramalin also showed inhibitory effect on LPS‐induced NF‐κB and MAPK activation. Overall, these finding indicate that ramalin suppresses autophagy in LPS‐stimulated macrophages by preventing NO production and downregulating both NF‐κB and MAPK activation. |
format |
Article in Journal/Newspaper |
author |
Park, Hye‐Jin Yim, Joung Han Lee, Hong Kum Pyo, Suhkneung |
spellingShingle |
Park, Hye‐Jin Yim, Joung Han Lee, Hong Kum Pyo, Suhkneung Ramalin inhibits LPS‐induced autophagic response in macrophages through the downregulation of iNOS expression |
author_facet |
Park, Hye‐Jin Yim, Joung Han Lee, Hong Kum Pyo, Suhkneung |
author_sort |
Park, Hye‐Jin |
title |
Ramalin inhibits LPS‐induced autophagic response in macrophages through the downregulation of iNOS expression |
title_short |
Ramalin inhibits LPS‐induced autophagic response in macrophages through the downregulation of iNOS expression |
title_full |
Ramalin inhibits LPS‐induced autophagic response in macrophages through the downregulation of iNOS expression |
title_fullStr |
Ramalin inhibits LPS‐induced autophagic response in macrophages through the downregulation of iNOS expression |
title_full_unstemmed |
Ramalin inhibits LPS‐induced autophagic response in macrophages through the downregulation of iNOS expression |
title_sort |
ramalin inhibits lps‐induced autophagic response in macrophages through the downregulation of inos expression |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1096/fasebj.27.1_supplement.888.2 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
The FASEB Journal volume 27, issue S1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.27.1_supplement.888.2 |
container_title |
The FASEB Journal |
container_volume |
27 |
container_issue |
S1 |
_version_ |
1800741517974831104 |