Gene expression analysis of healthy adults rapidly transported to high altitude

• Published in: The FASEB Journal
• Main Authors: Herman, Nicole Marie, Grill, Diane E, Anderson, Paul J, Miller, Andrew D, O'Malley, Kathy A, Johnson, Jacob B, Richert, Maile L Ceridon, Johnson, Bruce D
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2013
• Subjects: South Pole; McMurdo Station; Antarc*; Antarctica; South pole
• Online Access: http://dx.doi.org/10.1096/fasebj.27.1_supplement.715.1

Objective To identify novel genes that change expression in healthy adults exposed to hypobaric hypoxia. Background The uniform and rapid transfer of adults from McMurdo Station (sea level, SL) in Antarctica to the South Pole (2835m, SP) provides a unique opportunity to study the changes in gene expression related to the body's adaptation to high altitude. We hypothesized that a large number of genes would change expression and that many of these genes would be associated with inflammatory processes. Methods Peripheral venous blood was drawn at SL and after 2 d at SP in 98 subjects (age 37±9 y, 70% male). Peripheral blood mononuclear cells (PBMCs) were isolated and microarray analysis of the mRNA isolated from these cells was performed (Affymetrix HG U133 Plus 2 microarray chip). Analysis and the computation of expression fold changes was performed using the Bioconductor package in R. Pathway analysis was performed using MetaCore™. Results 1118 probe sets with significant p‐values (p<0.05) and fold changes were identified and entered into MetaCore™. Several pathways including oxidative phosphorylation, cytoskeletal remodeling, and platelet aggregation were identified as having significant representation in the restricted data set. Conclusion Several pathways indicating significantly increased activity in PBMCs were identified. This is indicative of an increased inflammatory response. NSF B‐179‐M.