Preserved PAR2 calcium signalling in endothelial cells of hypertensive mice
In hypertension where other signal transduction pathways are attenuated, protease‐activated receptor 2 (PAR2)‐mediated vascular relaxation is preserved. Despite this difference, PAR2 calcium events in vascular endothelium are not understood. We hypothesize that PAR2‐mediated calcium events are prese...
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crwiley:10.1096/fasebj.27.1_supplement.676.2 2024-06-02T08:10:44+00:00 Preserved PAR2 calcium signalling in endothelial cells of hypertensive mice Hennessey, John Charles Stuyvers, Bruno D McGuire, John J Canadian Institutes of Health Research 2013 http://dx.doi.org/10.1096/fasebj.27.1_supplement.676.2 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 27, issue S1 ISSN 0892-6638 1530-6860 journal-article 2013 crwiley https://doi.org/10.1096/fasebj.27.1_supplement.676.2 2024-05-03T10:44:13Z In hypertension where other signal transduction pathways are attenuated, protease‐activated receptor 2 (PAR2)‐mediated vascular relaxation is preserved. Despite this difference, PAR2 calcium events in vascular endothelium are not understood. We hypothesize that PAR2‐mediated calcium events are preserved in isolated mesenteric endothelial cells from hypertensive mice. Calcium activity was measured by spinning‐disk confocal microscopy in PAR2 wild type (PAR2‐WT) and PAR2 deficient (PAR2‐KO) mice treated subcutaneously with saline or angiotensin II (hypertensive). Isolated endothelial cells were incubated with fluorescent calcium indicator, fluo‐4 AM, and then exposed to a PAR2‐activating peptide 2‐furoyl LIGRLO‐amide (2fly) or muscarinic M 3 agonist acetylcholine. Our results show the number of calcium release sites and event rate are preserved in hypertensive PAR2‐WT exposed to 2fly. Acetylcholine‐induced calcium release sites and event rate were attenuated by 21% and 24% respectively. Similar results were found in hypertensive PAR2‐KO. Two types of calcium event were identified; small peripheral and repetitive, large central amplitude events. We conclude that PAR2‐mediated calcium signaling in isolated resistance endothelial cells is preserved in hypertension. Research funded by Canadian Institutes of Health Research and Research & Development Corporation Newfoundland and Labrador. Article in Journal/Newspaper Newfoundland Wiley Online Library Newfoundland The FASEB Journal 27 S1 |
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Wiley Online Library |
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English |
description |
In hypertension where other signal transduction pathways are attenuated, protease‐activated receptor 2 (PAR2)‐mediated vascular relaxation is preserved. Despite this difference, PAR2 calcium events in vascular endothelium are not understood. We hypothesize that PAR2‐mediated calcium events are preserved in isolated mesenteric endothelial cells from hypertensive mice. Calcium activity was measured by spinning‐disk confocal microscopy in PAR2 wild type (PAR2‐WT) and PAR2 deficient (PAR2‐KO) mice treated subcutaneously with saline or angiotensin II (hypertensive). Isolated endothelial cells were incubated with fluorescent calcium indicator, fluo‐4 AM, and then exposed to a PAR2‐activating peptide 2‐furoyl LIGRLO‐amide (2fly) or muscarinic M 3 agonist acetylcholine. Our results show the number of calcium release sites and event rate are preserved in hypertensive PAR2‐WT exposed to 2fly. Acetylcholine‐induced calcium release sites and event rate were attenuated by 21% and 24% respectively. Similar results were found in hypertensive PAR2‐KO. Two types of calcium event were identified; small peripheral and repetitive, large central amplitude events. We conclude that PAR2‐mediated calcium signaling in isolated resistance endothelial cells is preserved in hypertension. Research funded by Canadian Institutes of Health Research and Research & Development Corporation Newfoundland and Labrador. |
author2 |
Canadian Institutes of Health Research |
format |
Article in Journal/Newspaper |
author |
Hennessey, John Charles Stuyvers, Bruno D McGuire, John J |
spellingShingle |
Hennessey, John Charles Stuyvers, Bruno D McGuire, John J Preserved PAR2 calcium signalling in endothelial cells of hypertensive mice |
author_facet |
Hennessey, John Charles Stuyvers, Bruno D McGuire, John J |
author_sort |
Hennessey, John Charles |
title |
Preserved PAR2 calcium signalling in endothelial cells of hypertensive mice |
title_short |
Preserved PAR2 calcium signalling in endothelial cells of hypertensive mice |
title_full |
Preserved PAR2 calcium signalling in endothelial cells of hypertensive mice |
title_fullStr |
Preserved PAR2 calcium signalling in endothelial cells of hypertensive mice |
title_full_unstemmed |
Preserved PAR2 calcium signalling in endothelial cells of hypertensive mice |
title_sort |
preserved par2 calcium signalling in endothelial cells of hypertensive mice |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1096/fasebj.27.1_supplement.676.2 |
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Newfoundland |
geographic_facet |
Newfoundland |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
The FASEB Journal volume 27, issue S1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.27.1_supplement.676.2 |
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The FASEB Journal |
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27 |
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S1 |
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1800756650608427008 |