Effects of a Pharmacological Inhibitor on P‐38 MAPK in Drosophila melanogaster during Rapid Cold Hardening
Recent data suggests that p‐38 MAPK signaling may be involved in rapid cold hardening (RCH) of the house fly Musca Domestica , the Antarctic midge Belgica Antarctica , and the flesh fly Sarcophaga crassipalpis . Accordingly, this study used pharmacological techniques to determine whether p‐38 MAPK s...
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crwiley:10.1096/fasebj.26.1_supplement.1072.4 2024-06-02T07:58:07+00:00 Effects of a Pharmacological Inhibitor on P‐38 MAPK in Drosophila melanogaster during Rapid Cold Hardening Gardner, Daniel Dennis Kelty, Jonathan 2012 http://dx.doi.org/10.1096/fasebj.26.1_supplement.1072.4 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 26, issue S1 ISSN 0892-6638 1530-6860 journal-article 2012 crwiley https://doi.org/10.1096/fasebj.26.1_supplement.1072.4 2024-05-03T11:41:29Z Recent data suggests that p‐38 MAPK signaling may be involved in rapid cold hardening (RCH) of the house fly Musca Domestica , the Antarctic midge Belgica Antarctica , and the flesh fly Sarcophaga crassipalpis . Accordingly, this study used pharmacological techniques to determine whether p‐38 MAPK signaling is required for RCH in Drosophila melanogaster. As in prior studies, RCH afforded protection against cold shock injury for flies reared on drug‐free medium (yeast paste) for 48 h beginning within 6 h following eclosion; whereas no flies transferred directly from 23° C to −6° C for 1 h survived, 74% of those exposed to 1° C for 2 h immediately prior to a 1 h exposure to −6°C survived. By contrast, rearing flies on yeast paste containing 1 μg/mL SB 239063 impaired the capacity to rapidly cold harden; all flies fed SB 239063 were killed by a 1 h exposure to −6°C, regardless of whether they were first subjected to a RCH treatment. Similarly, preliminary data suggests that pharmacological inhibition of p38‐MAPK interferes with the protection of clinging behavior by rapid cold hardening. Article in Journal/Newspaper Antarc* Antarctic Antarctic midge Antarctica Belgica antarctica Wiley Online Library Antarctic The Antarctic The FASEB Journal 26 S1 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Recent data suggests that p‐38 MAPK signaling may be involved in rapid cold hardening (RCH) of the house fly Musca Domestica , the Antarctic midge Belgica Antarctica , and the flesh fly Sarcophaga crassipalpis . Accordingly, this study used pharmacological techniques to determine whether p‐38 MAPK signaling is required for RCH in Drosophila melanogaster. As in prior studies, RCH afforded protection against cold shock injury for flies reared on drug‐free medium (yeast paste) for 48 h beginning within 6 h following eclosion; whereas no flies transferred directly from 23° C to −6° C for 1 h survived, 74% of those exposed to 1° C for 2 h immediately prior to a 1 h exposure to −6°C survived. By contrast, rearing flies on yeast paste containing 1 μg/mL SB 239063 impaired the capacity to rapidly cold harden; all flies fed SB 239063 were killed by a 1 h exposure to −6°C, regardless of whether they were first subjected to a RCH treatment. Similarly, preliminary data suggests that pharmacological inhibition of p38‐MAPK interferes with the protection of clinging behavior by rapid cold hardening. |
format |
Article in Journal/Newspaper |
author |
Gardner, Daniel Dennis Kelty, Jonathan |
spellingShingle |
Gardner, Daniel Dennis Kelty, Jonathan Effects of a Pharmacological Inhibitor on P‐38 MAPK in Drosophila melanogaster during Rapid Cold Hardening |
author_facet |
Gardner, Daniel Dennis Kelty, Jonathan |
author_sort |
Gardner, Daniel Dennis |
title |
Effects of a Pharmacological Inhibitor on P‐38 MAPK in Drosophila melanogaster during Rapid Cold Hardening |
title_short |
Effects of a Pharmacological Inhibitor on P‐38 MAPK in Drosophila melanogaster during Rapid Cold Hardening |
title_full |
Effects of a Pharmacological Inhibitor on P‐38 MAPK in Drosophila melanogaster during Rapid Cold Hardening |
title_fullStr |
Effects of a Pharmacological Inhibitor on P‐38 MAPK in Drosophila melanogaster during Rapid Cold Hardening |
title_full_unstemmed |
Effects of a Pharmacological Inhibitor on P‐38 MAPK in Drosophila melanogaster during Rapid Cold Hardening |
title_sort |
effects of a pharmacological inhibitor on p‐38 mapk in drosophila melanogaster during rapid cold hardening |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1096/fasebj.26.1_supplement.1072.4 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic midge Antarctica Belgica antarctica |
genre_facet |
Antarc* Antarctic Antarctic midge Antarctica Belgica antarctica |
op_source |
The FASEB Journal volume 26, issue S1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.26.1_supplement.1072.4 |
container_title |
The FASEB Journal |
container_volume |
26 |
container_issue |
S1 |
_version_ |
1800741387815092224 |