Hypoxia inducible factor 1α, hypoxanthine‐guanine phosphoribolsyl transferase and NADPH oxidase 4 from northern elephant seals: cDNA characterization and tissue expression
Northern elephant seals ( Mirounga angustirostris ) are naturally adapted to prolonged periods (1–3 months) of food deprivation (fasting) and tissue ischemia. Fasting elephant seal pups do not exhibit signs of oxidative damage or inflammation despite increases in the renin‐angiotensin system, NADPH...
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crwiley:10.1096/fasebj.25.1_supplement.lb508 2024-06-02T08:06:00+00:00 Hypoxia inducible factor 1α, hypoxanthine‐guanine phosphoribolsyl transferase and NADPH oxidase 4 from northern elephant seals: cDNA characterization and tissue expression Organis, Jose Guadalupe Sonanez Medina, Jose Pablo Vazquez Aguilar, Andy Forman, Henry Crocker, Daniel Ortiz, Rudy 2011 http://dx.doi.org/10.1096/fasebj.25.1_supplement.lb508 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 25, issue S1 ISSN 0892-6638 1530-6860 journal-article 2011 crwiley https://doi.org/10.1096/fasebj.25.1_supplement.lb508 2024-05-03T11:53:27Z Northern elephant seals ( Mirounga angustirostris ) are naturally adapted to prolonged periods (1–3 months) of food deprivation (fasting) and tissue ischemia. Fasting elephant seal pups do not exhibit signs of oxidative damage or inflammation despite increases in the renin‐angiotensin system, NADPH oxidase activity and the superoxide‐generating enzyme, NADPH oxidase 4 (Nox4). Inducible factor 1α (HIF‐1α) and hypoxanthine‐guanine phosphoribosyl transferase (HGPRT) may be activated in response to fasting‐ and hypoxia‐induced increases in ROS. We report the molecular characterization and tissue expression of Nox4, HIF‐1α and HGPRT from fasting northern elephant seal pups. The full‐length sequence for HGPRT is 761 bp long and codes for 218 amino acids, which is similar to other mammalian homologs. The partial sequences of Nox4 and HIF‐1α are 1131 bp and 745 bp long respectively and correspond to approximately 65% and 35% of the full sequence of mammalian homologs, respectively. HGPRT, Nox4 and HIF‐1α sequences are similar to vertebrate homologs and contain conserved functional and regulatory domains. HGPRT, Nox4 and HIF‐1α expression was detected in both muscle and adipose tissue, with higher expression in muscle than in adipose. The present study represents a critical first step towards advancing our understanding of the molecular mechanisms of oxidant regulation in response to prolonged fasting and apnea‐induced ischemia. Article in Journal/Newspaper Elephant Seal Elephant Seals Wiley Online Library The FASEB Journal 25 S1 |
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Wiley Online Library |
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crwiley |
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English |
description |
Northern elephant seals ( Mirounga angustirostris ) are naturally adapted to prolonged periods (1–3 months) of food deprivation (fasting) and tissue ischemia. Fasting elephant seal pups do not exhibit signs of oxidative damage or inflammation despite increases in the renin‐angiotensin system, NADPH oxidase activity and the superoxide‐generating enzyme, NADPH oxidase 4 (Nox4). Inducible factor 1α (HIF‐1α) and hypoxanthine‐guanine phosphoribosyl transferase (HGPRT) may be activated in response to fasting‐ and hypoxia‐induced increases in ROS. We report the molecular characterization and tissue expression of Nox4, HIF‐1α and HGPRT from fasting northern elephant seal pups. The full‐length sequence for HGPRT is 761 bp long and codes for 218 amino acids, which is similar to other mammalian homologs. The partial sequences of Nox4 and HIF‐1α are 1131 bp and 745 bp long respectively and correspond to approximately 65% and 35% of the full sequence of mammalian homologs, respectively. HGPRT, Nox4 and HIF‐1α sequences are similar to vertebrate homologs and contain conserved functional and regulatory domains. HGPRT, Nox4 and HIF‐1α expression was detected in both muscle and adipose tissue, with higher expression in muscle than in adipose. The present study represents a critical first step towards advancing our understanding of the molecular mechanisms of oxidant regulation in response to prolonged fasting and apnea‐induced ischemia. |
format |
Article in Journal/Newspaper |
author |
Organis, Jose Guadalupe Sonanez Medina, Jose Pablo Vazquez Aguilar, Andy Forman, Henry Crocker, Daniel Ortiz, Rudy |
spellingShingle |
Organis, Jose Guadalupe Sonanez Medina, Jose Pablo Vazquez Aguilar, Andy Forman, Henry Crocker, Daniel Ortiz, Rudy Hypoxia inducible factor 1α, hypoxanthine‐guanine phosphoribolsyl transferase and NADPH oxidase 4 from northern elephant seals: cDNA characterization and tissue expression |
author_facet |
Organis, Jose Guadalupe Sonanez Medina, Jose Pablo Vazquez Aguilar, Andy Forman, Henry Crocker, Daniel Ortiz, Rudy |
author_sort |
Organis, Jose Guadalupe Sonanez |
title |
Hypoxia inducible factor 1α, hypoxanthine‐guanine phosphoribolsyl transferase and NADPH oxidase 4 from northern elephant seals: cDNA characterization and tissue expression |
title_short |
Hypoxia inducible factor 1α, hypoxanthine‐guanine phosphoribolsyl transferase and NADPH oxidase 4 from northern elephant seals: cDNA characterization and tissue expression |
title_full |
Hypoxia inducible factor 1α, hypoxanthine‐guanine phosphoribolsyl transferase and NADPH oxidase 4 from northern elephant seals: cDNA characterization and tissue expression |
title_fullStr |
Hypoxia inducible factor 1α, hypoxanthine‐guanine phosphoribolsyl transferase and NADPH oxidase 4 from northern elephant seals: cDNA characterization and tissue expression |
title_full_unstemmed |
Hypoxia inducible factor 1α, hypoxanthine‐guanine phosphoribolsyl transferase and NADPH oxidase 4 from northern elephant seals: cDNA characterization and tissue expression |
title_sort |
hypoxia inducible factor 1î±, hypoxanthine‐guanine phosphoribolsyl transferase and nadph oxidase 4 from northern elephant seals: cdna characterization and tissue expression |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1096/fasebj.25.1_supplement.lb508 |
genre |
Elephant Seal Elephant Seals |
genre_facet |
Elephant Seal Elephant Seals |
op_source |
The FASEB Journal volume 25, issue S1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.25.1_supplement.lb508 |
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The FASEB Journal |
container_volume |
25 |
container_issue |
S1 |
_version_ |
1800750885004902400 |