Tolerance of a choanoflagellate to environmental stressors
Choanoflagellates are the protozoan antecedents of multicellular animals, but little is known about their response to environmental change. Recently, the arctic choanoflagellate Diaphanoeca grandis was found in a supersulfidic, anoxic fjord in Norway, suggesting that at least some choanoflagellates...
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crwiley:10.1096/fasebj.22.1_supplement.648.21 2024-06-02T08:02:22+00:00 Tolerance of a choanoflagellate to environmental stressors Misiak, Kristen Dunlap, Kristen J Rodriguez, Sara S Julian, David 2008 http://dx.doi.org/10.1096/fasebj.22.1_supplement.648.21 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 22, issue S1 ISSN 0892-6638 1530-6860 journal-article 2008 crwiley https://doi.org/10.1096/fasebj.22.1_supplement.648.21 2024-05-03T11:29:32Z Choanoflagellates are the protozoan antecedents of multicellular animals, but little is known about their response to environmental change. Recently, the arctic choanoflagellate Diaphanoeca grandis was found in a supersulfidic, anoxic fjord in Norway, suggesting that at least some choanoflagellates are highly stress tolerant. We investigated this by testing the 24 h survival of D. grandis exposed to variations in pH (7.3–8.7), temperature (4, 10, 15, 20, 25, 30°C), Po 2 (normoxia vs. anoxia), salinity (13, 19, 26, 32, 48, 64‰), H 2 O 2 , (0.01, 0.1 and 1 mM) and H 2 S (0.03, 0.3, 1%). Cell death was assessed by morphology and propidium iodide labeling. Compared to control conditions (normoxia, 10°C, 32 ppt salinity, pH 8.0), cell death was significantly increased only at the highest pH (8.7, p = 0.023), temperatures of 20°C and above (p < 0.0001), the lowest and highest salinity (13 and 64 ppt; p < 0.0001), H 2 O 2 of 0.1 mM and above (p<0.0001), and H 2 S of 0.1% and above (p=0.0002). Furthermore, 65% of D. grandis were alive after 24 h of anoxia (p= 0.0023). These results indicate that D. grandis is surprisingly tolerant of typical environmental stressors. Whether this is characteristic of all choanoflagellates is still unknown. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Norway The FASEB Journal 22 S1 |
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English |
description |
Choanoflagellates are the protozoan antecedents of multicellular animals, but little is known about their response to environmental change. Recently, the arctic choanoflagellate Diaphanoeca grandis was found in a supersulfidic, anoxic fjord in Norway, suggesting that at least some choanoflagellates are highly stress tolerant. We investigated this by testing the 24 h survival of D. grandis exposed to variations in pH (7.3–8.7), temperature (4, 10, 15, 20, 25, 30°C), Po 2 (normoxia vs. anoxia), salinity (13, 19, 26, 32, 48, 64‰), H 2 O 2 , (0.01, 0.1 and 1 mM) and H 2 S (0.03, 0.3, 1%). Cell death was assessed by morphology and propidium iodide labeling. Compared to control conditions (normoxia, 10°C, 32 ppt salinity, pH 8.0), cell death was significantly increased only at the highest pH (8.7, p = 0.023), temperatures of 20°C and above (p < 0.0001), the lowest and highest salinity (13 and 64 ppt; p < 0.0001), H 2 O 2 of 0.1 mM and above (p<0.0001), and H 2 S of 0.1% and above (p=0.0002). Furthermore, 65% of D. grandis were alive after 24 h of anoxia (p= 0.0023). These results indicate that D. grandis is surprisingly tolerant of typical environmental stressors. Whether this is characteristic of all choanoflagellates is still unknown. |
format |
Article in Journal/Newspaper |
author |
Misiak, Kristen Dunlap, Kristen J Rodriguez, Sara S Julian, David |
spellingShingle |
Misiak, Kristen Dunlap, Kristen J Rodriguez, Sara S Julian, David Tolerance of a choanoflagellate to environmental stressors |
author_facet |
Misiak, Kristen Dunlap, Kristen J Rodriguez, Sara S Julian, David |
author_sort |
Misiak, Kristen |
title |
Tolerance of a choanoflagellate to environmental stressors |
title_short |
Tolerance of a choanoflagellate to environmental stressors |
title_full |
Tolerance of a choanoflagellate to environmental stressors |
title_fullStr |
Tolerance of a choanoflagellate to environmental stressors |
title_full_unstemmed |
Tolerance of a choanoflagellate to environmental stressors |
title_sort |
tolerance of a choanoflagellate to environmental stressors |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1096/fasebj.22.1_supplement.648.21 |
geographic |
Arctic Norway |
geographic_facet |
Arctic Norway |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
The FASEB Journal volume 22, issue S1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.22.1_supplement.648.21 |
container_title |
The FASEB Journal |
container_volume |
22 |
container_issue |
S1 |
_version_ |
1800746864245473280 |