Prolonged Fasting Increases DNA Methylation in Northern Elephant Seal Pups
Northern elephant seals (NES) are naturally exposed to extreme conditions, including prolonged food and water deprivation (fasting). NES pups initially nurse for a month before they are weaned and fast for two months. During this fasting period NES pups lose about 25% of their body mass, while maint...
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Online Access: | http://dx.doi.org/10.1096/fasebj.2020.34.s1.04334 |
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crwiley:10.1096/fasebj.2020.34.s1.04334 2024-06-02T08:06:01+00:00 Prolonged Fasting Increases DNA Methylation in Northern Elephant Seal Pups Gibson, Emily Torres-Velarde, Julia Maria Vazquez-Medina, Jose Pablo Crocker, Daniel 2020 http://dx.doi.org/10.1096/fasebj.2020.34.s1.04334 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 34, issue S1, page 1-1 ISSN 0892-6638 1530-6860 journal-article 2020 crwiley https://doi.org/10.1096/fasebj.2020.34.s1.04334 2024-05-03T11:11:21Z Northern elephant seals (NES) are naturally exposed to extreme conditions, including prolonged food and water deprivation (fasting). NES pups initially nurse for a month before they are weaned and fast for two months. During this fasting period NES pups lose about 25% of their body mass, while maintaining biochemical homeostasis and supporting muscle development. The environment, especially stressful environmental conditions, can modify the methylation status of DNA, consequently regulating gene expression. We compared global DNA methylation between early (1–2 weeks) and late (7–8 weeks) fasting NES pups. DNA was extracted from white blood cells collected from fasting NES pups. Global DNA methylation was measured using a MethylFlash™ Global DNA Methylation (5‐mC) Kit. Prolonged fasting significantly increased global DNA methylation (p = 0.0463) in NES pups, suggesting a decrease in transcription activity. We are in the process of identifying changes in specific genes involved in growth, hypoxia tolerance, and metabolism between early and late fasting pups by analyzing the methylation status of CpG islands in putative promoter region sequences. Our initial results suggest that DNA methylation is an important regulator of gene expression during natural, prolonged food deprivation. Article in Journal/Newspaper Elephant Seal Elephant Seals Wiley Online Library Nes ENVELOPE(7.634,7.634,62.795,62.795) Nes’ ENVELOPE(44.681,44.681,66.600,66.600) The FASEB Journal 34 S1 1 1 |
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Wiley Online Library |
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English |
description |
Northern elephant seals (NES) are naturally exposed to extreme conditions, including prolonged food and water deprivation (fasting). NES pups initially nurse for a month before they are weaned and fast for two months. During this fasting period NES pups lose about 25% of their body mass, while maintaining biochemical homeostasis and supporting muscle development. The environment, especially stressful environmental conditions, can modify the methylation status of DNA, consequently regulating gene expression. We compared global DNA methylation between early (1–2 weeks) and late (7–8 weeks) fasting NES pups. DNA was extracted from white blood cells collected from fasting NES pups. Global DNA methylation was measured using a MethylFlash™ Global DNA Methylation (5‐mC) Kit. Prolonged fasting significantly increased global DNA methylation (p = 0.0463) in NES pups, suggesting a decrease in transcription activity. We are in the process of identifying changes in specific genes involved in growth, hypoxia tolerance, and metabolism between early and late fasting pups by analyzing the methylation status of CpG islands in putative promoter region sequences. Our initial results suggest that DNA methylation is an important regulator of gene expression during natural, prolonged food deprivation. |
format |
Article in Journal/Newspaper |
author |
Gibson, Emily Torres-Velarde, Julia Maria Vazquez-Medina, Jose Pablo Crocker, Daniel |
spellingShingle |
Gibson, Emily Torres-Velarde, Julia Maria Vazquez-Medina, Jose Pablo Crocker, Daniel Prolonged Fasting Increases DNA Methylation in Northern Elephant Seal Pups |
author_facet |
Gibson, Emily Torres-Velarde, Julia Maria Vazquez-Medina, Jose Pablo Crocker, Daniel |
author_sort |
Gibson, Emily |
title |
Prolonged Fasting Increases DNA Methylation in Northern Elephant Seal Pups |
title_short |
Prolonged Fasting Increases DNA Methylation in Northern Elephant Seal Pups |
title_full |
Prolonged Fasting Increases DNA Methylation in Northern Elephant Seal Pups |
title_fullStr |
Prolonged Fasting Increases DNA Methylation in Northern Elephant Seal Pups |
title_full_unstemmed |
Prolonged Fasting Increases DNA Methylation in Northern Elephant Seal Pups |
title_sort |
prolonged fasting increases dna methylation in northern elephant seal pups |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1096/fasebj.2020.34.s1.04334 |
long_lat |
ENVELOPE(7.634,7.634,62.795,62.795) ENVELOPE(44.681,44.681,66.600,66.600) |
geographic |
Nes Nes’ |
geographic_facet |
Nes Nes’ |
genre |
Elephant Seal Elephant Seals |
genre_facet |
Elephant Seal Elephant Seals |
op_source |
The FASEB Journal volume 34, issue S1, page 1-1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.2020.34.s1.04334 |
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The FASEB Journal |
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34 |
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S1 |
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1 |
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1 |
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1800750892288311296 |