Homarus Gemmarus: Seriated CT‐Scan and Re‐Constructions: Horizontal, Sagittal and 3D

• Published in: The FASEB Journal
• Main Authors: le floch‐prigent, patrice pierre, Verdeille, stéphane
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2018
• Subjects: European lobster; Homarus gammarus
• Online Access: http://dx.doi.org/10.1096/fasebj.2018.32.1_supplement.lb535; https://onlinelibrary.wiley.com/doi/full-xml/10.1096/fasebj.2018.32.1_supplement.lb535; https://onlinelibrary.wiley.com/doi/full/10.1096/fasebj.2018.32.1_supplement.lb535

The european lobster is a large, common, marine, crustacean decapod. Material An entire animal was acquired still alive and deeply frozen in extension of the abdomen. It was 26,5 cm long; 7 cm width at its maximal dimension of the cephalo‐thorax. The CT‐scan apparatus was a Siemens somatom sensitive, whole body apparatus with 128 stellar detectors. Methods The entire animal was CT‐scanned all along the rostro‐caudal axis, in axial cross‐sections and reconstructed in the two other planes (horizontal and para‐sagittal) and in 3‐ dimensional ones. The frozen lobster was laid in prone position on the radiological table. It was CT‐scanned with an acquisition (100 kV, 18 mAs) in the axial plane: the axial cross‐sections (every 0,8 mm, 1 mm thick) were joined, and frontal. Two different penetrations were used: B30 for the soft parts and B70 for the hard parts (cartilage and extra‐skeleton). Results 500 frontal cross‐sections were obtained from the native axial acquisition. 120 para‐sagittal cross‐sections were reconstructed from the preceding ones, and 80 horizontal ones as well. 11, 3D reconstructions were realized with several incidences: superior, inferior, rostral, caudal, postero‐superior, antero‐inferior, antero‐lateral and superior (right and left), lateral (right and left), median sagittal. Conclusion The seriated CT‐scan and the reconstructions afforded very numerous morphological elements in Homarus gammarus , and also in the study of their relationships. The muscular masses presented very few differenciation. The rare cartilaginous structures were well vizualized. A voluminous aerial poach at the upper and rostral part of the cephalo‐thorax corresponded to the two parts of the stomach: cardial (rostral) and pyloric (caudal) ones. The reconstructions were very contributive. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .