Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla
Abstract In a study of reproduction in male European eels Anguilla anguilla , we induced spermatogenesis through hormone injection and established a spermatogenic maturity index (SMI) as a novel quantification of testis development. Eels in the experiments were sacrificed weekly and testis tissue wa...
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Online Access: | http://dx.doi.org/10.1080/19425120.2011.556902 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/19425120.2011.556902 |
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crwiley:10.1080/19425120.2011.556902 2024-06-23T07:45:32+00:00 Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla Tomkiewicz, Jonna Kofoed, Tanja M. N. Pedersen, Jes S. 2011 http://dx.doi.org/10.1080/19425120.2011.556902 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/19425120.2011.556902 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Marine and Coastal Fisheries volume 3, issue 1, page 106-118 ISSN 1942-5120 1942-5120 journal-article 2011 crwiley https://doi.org/10.1080/19425120.2011.556902 2024-06-04T06:38:23Z Abstract In a study of reproduction in male European eels Anguilla anguilla , we induced spermatogenesis through hormone injection and established a spermatogenic maturity index (SMI) as a novel quantification of testis development. Eels in the experiments were sacrificed weekly and testis tissue was sampled for histological analysis of spermatogenesis. Testis development was followed over 18 weeks, during which the males continued to develop spermatocytes and produce spermatozoa. The SMI describes testis development from estimation of the area fractions of various tissue categories characterized by progressive gamete development stages in histological sections of the testes. The index weighs the volume fractions of the different tissues (somatic cells and germ cell stages) and describes development on a scale of 0 to 1. The method improves the existing histological classification, providing a quantitative measure that reflects the spermatogenic process and can be correlated with morphological and physiological parameters. In this study, the SMI reacted immediately to the onset of spermatogenesis and increased linearly over time, tracking the development of spermatocysts and spermatozoa. In week 7, the SMI reached a stable level of around 0.75, where it remained, with limited fluctuations, until the end of the experiment. This reflected the composition of different germ cell stages in the testis tissue with a continuous generation of spermatocysts and production of spermatozoa. In comparison, the gonadosomatic index showed a delayed response to the onset of spermatogenesis and fluctuated substantially during the sperm production period. The properties of the SMI made it a useful index for describing spermatogenesis in male European eels during this experiment and a promising tool for quantifying testis development and describing male reproductive strategy in other fish species. Article in Journal/Newspaper Anguilla anguilla European eel Wiley Online Library Marine and Coastal Fisheries 3 1 106 118 |
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English |
description |
Abstract In a study of reproduction in male European eels Anguilla anguilla , we induced spermatogenesis through hormone injection and established a spermatogenic maturity index (SMI) as a novel quantification of testis development. Eels in the experiments were sacrificed weekly and testis tissue was sampled for histological analysis of spermatogenesis. Testis development was followed over 18 weeks, during which the males continued to develop spermatocytes and produce spermatozoa. The SMI describes testis development from estimation of the area fractions of various tissue categories characterized by progressive gamete development stages in histological sections of the testes. The index weighs the volume fractions of the different tissues (somatic cells and germ cell stages) and describes development on a scale of 0 to 1. The method improves the existing histological classification, providing a quantitative measure that reflects the spermatogenic process and can be correlated with morphological and physiological parameters. In this study, the SMI reacted immediately to the onset of spermatogenesis and increased linearly over time, tracking the development of spermatocysts and spermatozoa. In week 7, the SMI reached a stable level of around 0.75, where it remained, with limited fluctuations, until the end of the experiment. This reflected the composition of different germ cell stages in the testis tissue with a continuous generation of spermatocysts and production of spermatozoa. In comparison, the gonadosomatic index showed a delayed response to the onset of spermatogenesis and fluctuated substantially during the sperm production period. The properties of the SMI made it a useful index for describing spermatogenesis in male European eels during this experiment and a promising tool for quantifying testis development and describing male reproductive strategy in other fish species. |
format |
Article in Journal/Newspaper |
author |
Tomkiewicz, Jonna Kofoed, Tanja M. N. Pedersen, Jes S. |
spellingShingle |
Tomkiewicz, Jonna Kofoed, Tanja M. N. Pedersen, Jes S. Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
author_facet |
Tomkiewicz, Jonna Kofoed, Tanja M. N. Pedersen, Jes S. |
author_sort |
Tomkiewicz, Jonna |
title |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_short |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_full |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_fullStr |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_full_unstemmed |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_sort |
assessment of testis development during induced spermatogenesis in the european eel anguilla anguilla |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1080/19425120.2011.556902 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/19425120.2011.556902 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Marine and Coastal Fisheries volume 3, issue 1, page 106-118 ISSN 1942-5120 1942-5120 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1080/19425120.2011.556902 |
container_title |
Marine and Coastal Fisheries |
container_volume |
3 |
container_issue |
1 |
container_start_page |
106 |
op_container_end_page |
118 |
_version_ |
1802640628405764096 |