Cold‐adapted signal proteins: NMR structures of pheromones from the antarctic ciliate Euplotes nobilii
Abstract Cell type‐specific signal proteins, known as pheromones, are synthesized by ciliated protozoa in association with their self/nonself mating‐type systems, and are utilized to control the vegetative growth and mating stages of their life cycle. In species of the most ubiquitous ciliate, Euplo...
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crwiley:10.1080/15216540701258165 2024-06-23T07:47:07+00:00 Cold‐adapted signal proteins: NMR structures of pheromones from the antarctic ciliate Euplotes nobilii Placzek, William J. Etezady‐Esfarjani, Touraj Herrmann, Torsten Pedrini, Bill Peti, Wolfgang Alimenti, Claudio Luporini, Pierangelo Wüthrich, Kurt 2007 http://dx.doi.org/10.1080/15216540701258165 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1080%2F15216540701258165 https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1080/15216540701258165 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor IUBMB Life volume 59, issue 8-9, page 578-585 ISSN 1521-6543 1521-6551 journal-article 2007 crwiley https://doi.org/10.1080/15216540701258165 2024-06-13T04:23:58Z Abstract Cell type‐specific signal proteins, known as pheromones, are synthesized by ciliated protozoa in association with their self/nonself mating‐type systems, and are utilized to control the vegetative growth and mating stages of their life cycle. In species of the most ubiquitous ciliate, Euplotes, these pheromones form families of structurally homologous molecules, which are constitutively secreted into the extracellular environment, from where they can be isolated in sufficient amounts for chemical characterization. This paper describes the NMR structures of En‐1 and En‐2, which are members of the cold‐adapted pheromone family produced by Euplotes nobilii, a species inhabiting the freezing coastal waters of Antarctica. The structures were determined with the proteins from the natural source, using homonuclear 1H NMR techniques in combination with automated NOESY peak picking and NOE assignment. En‐1 and En‐2 have highly homologous global folds, which consist of a central three‐α‐helix bundle with an up‐down‐up topology and a 310‐helical turn near the N‐terminus. This fold is stabilized by four disulfide bonds and the helices are connected by bulging loops. Apparent structural specificity resides in the variable C‐terminal regions of the pheromones. The NMR structures of En‐1 and En‐2 provide novel insights into the cold‐adaptive modifications that distinguish the E. nobilii pheromone family from the closely related E. raikovi pheromone family isolated from temperate waters. Article in Journal/Newspaper Antarc* Antarctic Antarctica Wiley Online Library Antarctic The Antarctic IUBMB Life 59 8 578 585 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Cell type‐specific signal proteins, known as pheromones, are synthesized by ciliated protozoa in association with their self/nonself mating‐type systems, and are utilized to control the vegetative growth and mating stages of their life cycle. In species of the most ubiquitous ciliate, Euplotes, these pheromones form families of structurally homologous molecules, which are constitutively secreted into the extracellular environment, from where they can be isolated in sufficient amounts for chemical characterization. This paper describes the NMR structures of En‐1 and En‐2, which are members of the cold‐adapted pheromone family produced by Euplotes nobilii, a species inhabiting the freezing coastal waters of Antarctica. The structures were determined with the proteins from the natural source, using homonuclear 1H NMR techniques in combination with automated NOESY peak picking and NOE assignment. En‐1 and En‐2 have highly homologous global folds, which consist of a central three‐α‐helix bundle with an up‐down‐up topology and a 310‐helical turn near the N‐terminus. This fold is stabilized by four disulfide bonds and the helices are connected by bulging loops. Apparent structural specificity resides in the variable C‐terminal regions of the pheromones. The NMR structures of En‐1 and En‐2 provide novel insights into the cold‐adaptive modifications that distinguish the E. nobilii pheromone family from the closely related E. raikovi pheromone family isolated from temperate waters. |
format |
Article in Journal/Newspaper |
author |
Placzek, William J. Etezady‐Esfarjani, Touraj Herrmann, Torsten Pedrini, Bill Peti, Wolfgang Alimenti, Claudio Luporini, Pierangelo Wüthrich, Kurt |
spellingShingle |
Placzek, William J. Etezady‐Esfarjani, Touraj Herrmann, Torsten Pedrini, Bill Peti, Wolfgang Alimenti, Claudio Luporini, Pierangelo Wüthrich, Kurt Cold‐adapted signal proteins: NMR structures of pheromones from the antarctic ciliate Euplotes nobilii |
author_facet |
Placzek, William J. Etezady‐Esfarjani, Touraj Herrmann, Torsten Pedrini, Bill Peti, Wolfgang Alimenti, Claudio Luporini, Pierangelo Wüthrich, Kurt |
author_sort |
Placzek, William J. |
title |
Cold‐adapted signal proteins: NMR structures of pheromones from the antarctic ciliate Euplotes nobilii |
title_short |
Cold‐adapted signal proteins: NMR structures of pheromones from the antarctic ciliate Euplotes nobilii |
title_full |
Cold‐adapted signal proteins: NMR structures of pheromones from the antarctic ciliate Euplotes nobilii |
title_fullStr |
Cold‐adapted signal proteins: NMR structures of pheromones from the antarctic ciliate Euplotes nobilii |
title_full_unstemmed |
Cold‐adapted signal proteins: NMR structures of pheromones from the antarctic ciliate Euplotes nobilii |
title_sort |
cold‐adapted signal proteins: nmr structures of pheromones from the antarctic ciliate euplotes nobilii |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1080/15216540701258165 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1080%2F15216540701258165 https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1080/15216540701258165 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
IUBMB Life volume 59, issue 8-9, page 578-585 ISSN 1521-6543 1521-6551 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1080/15216540701258165 |
container_title |
IUBMB Life |
container_volume |
59 |
container_issue |
8 |
container_start_page |
578 |
op_container_end_page |
585 |
_version_ |
1802651021064798208 |