Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout
Abstract A novel herpesvirus was found by molecular methods in samples of Lake Trout Salvelinus namaycush from Lake Erie, Pennsylvania, and Lake Ontario, Keuka Lake, and Lake Otsego, New York. Based on PCR amplification and partial sequencing of polymerase, terminase, and glycoprotein genes, a numbe...
Published in: | Journal of Aquatic Animal Health |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2016
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/08997659.2015.1111270 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2015.1111270 |
id |
crwiley:10.1080/08997659.2015.1111270 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1080/08997659.2015.1111270 2024-06-02T08:03:32+00:00 Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout Glenney, Gavin W. Barbash, Patricia A. Coll, John A. 2016 http://dx.doi.org/10.1080/08997659.2015.1111270 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2015.1111270 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Aquatic Animal Health volume 28, issue 1, page 46-55 ISSN 0899-7659 1548-8667 journal-article 2016 crwiley https://doi.org/10.1080/08997659.2015.1111270 2024-05-03T10:54:18Z Abstract A novel herpesvirus was found by molecular methods in samples of Lake Trout Salvelinus namaycush from Lake Erie, Pennsylvania, and Lake Ontario, Keuka Lake, and Lake Otsego, New York. Based on PCR amplification and partial sequencing of polymerase, terminase, and glycoprotein genes, a number of isolates were identified as a novel virus, which we have named Namaycush herpesvirus (NamHV) salmonid herpesvirus 5 (SalHV5). Phylogenetic analyses of three NamHV genes indicated strong clustering with other members of the genus Salmonivirus , placing these isolates into family Alloherpesviridae. The NamHV isolates were identical in the three partially sequenced genes; however, they varied from other salmonid herpesviruses in nucleotide sequence identity. In all three of the genes sequenced, NamHV shared the highest sequence identity with Atlantic Salmon papillomatosis virus (ASPV; SalHV4) isolated from Atlantic Salmon Salmo salar in northern Europe, including northwestern Russia. These results lead one to believe that NamHV and ASPV have a common ancestor that may have made a relatively recent host jump from Atlantic Salmon to Lake Trout or vice versa. Partial nucleotide sequence comparisons between NamHV and ASPV for the polymerase and glycoprotein genes differ by >5% and >10%, respectively. Additional nucleotide sequence comparisons between NamHV and epizootic epitheliotropic disease virus (EEDV/SalHV3) in the terminase, glycoprotein, and polymerase genes differ by >5%, >20%, and >10%, respectively. Thus, NamHV and EEDV may be occupying discrete ecological niches in Lake Trout. Even though NamHV shared the highest genetic identity with ASPV, each of these viruses has a separate host species, which also implies speciation. Additionally, NamHV has been detected over the last 4 years in four separate water bodies across two states, which suggests that NamHV is a distinct, naturally replicating lineage. This, in combination with a divergence in nucleotide sequence from EEDV, indicates that NamHV is ... Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Aquatic Animal Health 28 1 46 55 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract A novel herpesvirus was found by molecular methods in samples of Lake Trout Salvelinus namaycush from Lake Erie, Pennsylvania, and Lake Ontario, Keuka Lake, and Lake Otsego, New York. Based on PCR amplification and partial sequencing of polymerase, terminase, and glycoprotein genes, a number of isolates were identified as a novel virus, which we have named Namaycush herpesvirus (NamHV) salmonid herpesvirus 5 (SalHV5). Phylogenetic analyses of three NamHV genes indicated strong clustering with other members of the genus Salmonivirus , placing these isolates into family Alloherpesviridae. The NamHV isolates were identical in the three partially sequenced genes; however, they varied from other salmonid herpesviruses in nucleotide sequence identity. In all three of the genes sequenced, NamHV shared the highest sequence identity with Atlantic Salmon papillomatosis virus (ASPV; SalHV4) isolated from Atlantic Salmon Salmo salar in northern Europe, including northwestern Russia. These results lead one to believe that NamHV and ASPV have a common ancestor that may have made a relatively recent host jump from Atlantic Salmon to Lake Trout or vice versa. Partial nucleotide sequence comparisons between NamHV and ASPV for the polymerase and glycoprotein genes differ by >5% and >10%, respectively. Additional nucleotide sequence comparisons between NamHV and epizootic epitheliotropic disease virus (EEDV/SalHV3) in the terminase, glycoprotein, and polymerase genes differ by >5%, >20%, and >10%, respectively. Thus, NamHV and EEDV may be occupying discrete ecological niches in Lake Trout. Even though NamHV shared the highest genetic identity with ASPV, each of these viruses has a separate host species, which also implies speciation. Additionally, NamHV has been detected over the last 4 years in four separate water bodies across two states, which suggests that NamHV is a distinct, naturally replicating lineage. This, in combination with a divergence in nucleotide sequence from EEDV, indicates that NamHV is ... |
format |
Article in Journal/Newspaper |
author |
Glenney, Gavin W. Barbash, Patricia A. Coll, John A. |
spellingShingle |
Glenney, Gavin W. Barbash, Patricia A. Coll, John A. Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout |
author_facet |
Glenney, Gavin W. Barbash, Patricia A. Coll, John A. |
author_sort |
Glenney, Gavin W. |
title |
Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout |
title_short |
Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout |
title_full |
Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout |
title_fullStr |
Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout |
title_full_unstemmed |
Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout |
title_sort |
initial detection and molecular characterization of namaycush herpesvirus (salmonid herpesvirus 5) in lake trout |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1080/08997659.2015.1111270 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2015.1111270 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Aquatic Animal Health volume 28, issue 1, page 46-55 ISSN 0899-7659 1548-8667 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1080/08997659.2015.1111270 |
container_title |
Journal of Aquatic Animal Health |
container_volume |
28 |
container_issue |
1 |
container_start_page |
46 |
op_container_end_page |
55 |
_version_ |
1800748089234948096 |