Evaluation on Activity of Cytochrome P450 Enzymes in Turbot Via a Probe Drug Cocktail
Abstract Cytochrome P450s (CYPs) are the main catalytic enzymes for metabolism by a variety of endogenous and exogenous substrates in mammals, fish, insects, etc. We evaluated the application of a multidrug cocktail on changes in CYP1, CYP2, and CYP3 activity in Turbot Scophthalmus maximus . The pro...
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crwiley:10.1080/08997659.2014.938868 2024-06-02T08:14:11+00:00 Evaluation on Activity of Cytochrome P450 Enzymes in Turbot Via a Probe Drug Cocktail Chang, Zhi‐Qiang Li, Jian Zhai, Qian‐Qian Chinese Academy of Fishery Sciences 2014 http://dx.doi.org/10.1080/08997659.2014.938868 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2014.938868 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Aquatic Animal Health volume 26, issue 4, page 272-277 ISSN 0899-7659 1548-8667 journal-article 2014 crwiley https://doi.org/10.1080/08997659.2014.938868 2024-05-03T11:12:55Z Abstract Cytochrome P450s (CYPs) are the main catalytic enzymes for metabolism by a variety of endogenous and exogenous substrates in mammals, fish, insects, etc. We evaluated the application of a multidrug cocktail on changes in CYP1, CYP2, and CYP3 activity in Turbot Scophthalmus maximus . The probe drugs were a combination of caffeine (5 mg/kg body weight), dapsone (5 mg/kg), and chlorzoxazone (10 mg/kg). After a single intraperitoneal injection of the cocktail, the concentration of all three probe drugs in the plasma increased quickly to a peak and then decreased gradually over 24 h. Pharmacokinetic profiles of the three probe drugs were determined using a noncompartmental analysis, and the typical parameters were calculated. In the assay for CYP induction, pretreatment with rifampicin significantly reduced the typical pharmacokinetic metrics for caffeine and chlorzoxazone, but not dapsone, indicating that the activity of CYP1 and CYP2 in turbot were induced by rifampicin. Received November 25, 2013; accepted May 29, 2014. Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library Journal of Aquatic Animal Health 26 4 272 277 |
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Wiley Online Library |
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English |
description |
Abstract Cytochrome P450s (CYPs) are the main catalytic enzymes for metabolism by a variety of endogenous and exogenous substrates in mammals, fish, insects, etc. We evaluated the application of a multidrug cocktail on changes in CYP1, CYP2, and CYP3 activity in Turbot Scophthalmus maximus . The probe drugs were a combination of caffeine (5 mg/kg body weight), dapsone (5 mg/kg), and chlorzoxazone (10 mg/kg). After a single intraperitoneal injection of the cocktail, the concentration of all three probe drugs in the plasma increased quickly to a peak and then decreased gradually over 24 h. Pharmacokinetic profiles of the three probe drugs were determined using a noncompartmental analysis, and the typical parameters were calculated. In the assay for CYP induction, pretreatment with rifampicin significantly reduced the typical pharmacokinetic metrics for caffeine and chlorzoxazone, but not dapsone, indicating that the activity of CYP1 and CYP2 in turbot were induced by rifampicin. Received November 25, 2013; accepted May 29, 2014. |
author2 |
Chinese Academy of Fishery Sciences |
format |
Article in Journal/Newspaper |
author |
Chang, Zhi‐Qiang Li, Jian Zhai, Qian‐Qian |
spellingShingle |
Chang, Zhi‐Qiang Li, Jian Zhai, Qian‐Qian Evaluation on Activity of Cytochrome P450 Enzymes in Turbot Via a Probe Drug Cocktail |
author_facet |
Chang, Zhi‐Qiang Li, Jian Zhai, Qian‐Qian |
author_sort |
Chang, Zhi‐Qiang |
title |
Evaluation on Activity of Cytochrome P450 Enzymes in Turbot Via a Probe Drug Cocktail |
title_short |
Evaluation on Activity of Cytochrome P450 Enzymes in Turbot Via a Probe Drug Cocktail |
title_full |
Evaluation on Activity of Cytochrome P450 Enzymes in Turbot Via a Probe Drug Cocktail |
title_fullStr |
Evaluation on Activity of Cytochrome P450 Enzymes in Turbot Via a Probe Drug Cocktail |
title_full_unstemmed |
Evaluation on Activity of Cytochrome P450 Enzymes in Turbot Via a Probe Drug Cocktail |
title_sort |
evaluation on activity of cytochrome p450 enzymes in turbot via a probe drug cocktail |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1080/08997659.2014.938868 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2014.938868 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Journal of Aquatic Animal Health volume 26, issue 4, page 272-277 ISSN 0899-7659 1548-8667 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1080/08997659.2014.938868 |
container_title |
Journal of Aquatic Animal Health |
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26 |
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4 |
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272 |
op_container_end_page |
277 |
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1800737902931476480 |