Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters
Abstract Preventing vibriosis in juvenile cultured Turbot Scophthalmus maximus caused by Vibrio anguillarum frequently requires the use of feed supplemented with antibiotics in addition to vaccines. Whether the use of probiotics instead of antibiotics in juvenile Turbot is a safer strategy requires...
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crwiley:10.1080/08997659.2014.920734 2024-06-02T08:14:10+00:00 Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters Farto Seguín, Rosa M. Bermúdez, María Bobo Rivera, Leticia Nieto, Teresa P. 2014 http://dx.doi.org/10.1080/08997659.2014.920734 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2014.920734 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Aquatic Animal Health volume 26, issue 4, page 251-262 ISSN 0899-7659 1548-8667 journal-article 2014 crwiley https://doi.org/10.1080/08997659.2014.920734 2024-05-03T11:01:02Z Abstract Preventing vibriosis in juvenile cultured Turbot Scophthalmus maximus caused by Vibrio anguillarum frequently requires the use of feed supplemented with antibiotics in addition to vaccines. Whether the use of probiotics instead of antibiotics in juvenile Turbot is a safer strategy requires more study. The antibacterial potential of 148 Vibrio spp. strains (mostly isolated from cultures of healthy oysters, clams, and Turbot) was analyzed in vitro against V. anguillarum and other pathogens by means of an agar diffusion assay. A wide spectrum of inhibitory activity was shown by 9 strains. Based on their easy phenotypic differentiation from V. anguillarum , we selected two strains (S1 and S2, both isolated from the European flat oyster Ostrea edulis ) for testing in juvenile Turbot (3 g). None of the strains were virulent by intraperitoneal or bath challenges, and all were susceptible to the antibiotics most frequently used in aquaculture. Three different stocks of Turbot, which were assayed separately, were significantly protected from infection with V. anguillarum . The final survival rates of fish treated in mixed challenges with S1 or S2 and V. anguillarum were 44% and 66%, respectively, whereas only 17% of the fish treated with only the pathogenic strain survived. The application of probiotic strains also increased the survival time of juvenile Turbot after infection with V. anguillarum. Both strains persisted in the epidermal mucus layer of the fish for 30 d, and they were not displaced by the pathogen. These data prove the efficacy of using bacteria well adapted to the dynamics of culture production as a way to provide juvenile Turbot immediate protection against infection by V. anguillarum. Moreover, the epidermal mucus sampling was useful for investigating the persistence of both probiotic strains when exposed to the pathogen. Received October 11, 2013; accepted March 10, 2014 Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library Journal of Aquatic Animal Health 26 4 251 262 |
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Wiley Online Library |
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English |
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Abstract Preventing vibriosis in juvenile cultured Turbot Scophthalmus maximus caused by Vibrio anguillarum frequently requires the use of feed supplemented with antibiotics in addition to vaccines. Whether the use of probiotics instead of antibiotics in juvenile Turbot is a safer strategy requires more study. The antibacterial potential of 148 Vibrio spp. strains (mostly isolated from cultures of healthy oysters, clams, and Turbot) was analyzed in vitro against V. anguillarum and other pathogens by means of an agar diffusion assay. A wide spectrum of inhibitory activity was shown by 9 strains. Based on their easy phenotypic differentiation from V. anguillarum , we selected two strains (S1 and S2, both isolated from the European flat oyster Ostrea edulis ) for testing in juvenile Turbot (3 g). None of the strains were virulent by intraperitoneal or bath challenges, and all were susceptible to the antibiotics most frequently used in aquaculture. Three different stocks of Turbot, which were assayed separately, were significantly protected from infection with V. anguillarum . The final survival rates of fish treated in mixed challenges with S1 or S2 and V. anguillarum were 44% and 66%, respectively, whereas only 17% of the fish treated with only the pathogenic strain survived. The application of probiotic strains also increased the survival time of juvenile Turbot after infection with V. anguillarum. Both strains persisted in the epidermal mucus layer of the fish for 30 d, and they were not displaced by the pathogen. These data prove the efficacy of using bacteria well adapted to the dynamics of culture production as a way to provide juvenile Turbot immediate protection against infection by V. anguillarum. Moreover, the epidermal mucus sampling was useful for investigating the persistence of both probiotic strains when exposed to the pathogen. Received October 11, 2013; accepted March 10, 2014 |
format |
Article in Journal/Newspaper |
author |
Farto Seguín, Rosa M. Bermúdez, María Bobo Rivera, Leticia Nieto, Teresa P. |
spellingShingle |
Farto Seguín, Rosa M. Bermúdez, María Bobo Rivera, Leticia Nieto, Teresa P. Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
author_facet |
Farto Seguín, Rosa M. Bermúdez, María Bobo Rivera, Leticia Nieto, Teresa P. |
author_sort |
Farto Seguín, Rosa M. |
title |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_short |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_full |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_fullStr |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_full_unstemmed |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_sort |
increased survival of juvenile turbot scophthalmus maximus by using bacteria associated with cultured oysters |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1080/08997659.2014.920734 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2014.920734 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Journal of Aquatic Animal Health volume 26, issue 4, page 251-262 ISSN 0899-7659 1548-8667 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1080/08997659.2014.920734 |
container_title |
Journal of Aquatic Animal Health |
container_volume |
26 |
container_issue |
4 |
container_start_page |
251 |
op_container_end_page |
262 |
_version_ |
1800737898041966592 |