Molecular Characterization of the VP2 Gene of Infectious Pancreatic Necrosis Virus (IPNV) Isolates from Mexico
Abstract Infectious pancreatic necrosis virus (IPNV) is one of the most important viruses in the Pacific salmon Oncorhynchus spp., Atlantic Salmon Salmo salar , and Rainbow Trout O. mykiss industry. This virus has been shown to produce high mortality among salmonid fry and juveniles, and survivors m...
Published in: | Journal of Aquatic Animal Health |
---|---|
Main Authors: | , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2014
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/08997659.2013.860060 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2013.860060 |
id |
crwiley:10.1080/08997659.2013.860060 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1080/08997659.2013.860060 2024-06-02T08:03:42+00:00 Molecular Characterization of the VP2 Gene of Infectious Pancreatic Necrosis Virus (IPNV) Isolates from Mexico Salgado‐Miranda, Celene Rojas‐Anaya, Edith García‐Espinosa, Gary Loza‐Rubio, Elizabeth Consejo Nacional de Ciencia y Tecnología 2014 http://dx.doi.org/10.1080/08997659.2013.860060 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2013.860060 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Aquatic Animal Health volume 26, issue 1, page 43-51 ISSN 0899-7659 1548-8667 journal-article 2014 crwiley https://doi.org/10.1080/08997659.2013.860060 2024-05-03T12:04:07Z Abstract Infectious pancreatic necrosis virus (IPNV) is one of the most important viruses in the Pacific salmon Oncorhynchus spp., Atlantic Salmon Salmo salar , and Rainbow Trout O. mykiss industry. This virus has been shown to produce high mortality among salmonid fry and juveniles, and survivors might become carriers. Since 2000, IPNV has affected Mexican Rainbow Trout culture, resulting in considerable economic losses. In the current study, molecular characterization of the VP2 gene of a number of Mexican IPNV isolates was done and the virus's phylogenetic relationships to IPNV reference strains were investigated. The phylogenetic analysis indicated that Mexican IPNV isolates are closely related to strains from the United States and Canada and that all Mexican IPNV isolates belong to genogroup 1. Furthermore, low genetic diversity was found between the Mexican isolates (identity, 95.8–99.8% nucleotides and 95.8–99.6% aminoacids). The result of the analysis of the amino acid residues found at positions 217, 221, and 247 (alanine, threonine, and glutamic acid, respectively) could be associated with virulence, although the expression of virulence factors is more complex and may be influenced by the agent and host factors. The high percentage of identity among the VP2 genes from geographically distant IPNV isolates and the evidence of wide distribution in the country might have been facilitated by carrier trout. This hypothesis is supported by the identification of the amino acid threonine at position 221 in all Mexican isolates, a factor related to the carrier state for IPNV, as reported by other studies. Received July 14, 2013; accepted October 24, 2013 Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Canada Pacific Journal of Aquatic Animal Health 26 1 43 51 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Infectious pancreatic necrosis virus (IPNV) is one of the most important viruses in the Pacific salmon Oncorhynchus spp., Atlantic Salmon Salmo salar , and Rainbow Trout O. mykiss industry. This virus has been shown to produce high mortality among salmonid fry and juveniles, and survivors might become carriers. Since 2000, IPNV has affected Mexican Rainbow Trout culture, resulting in considerable economic losses. In the current study, molecular characterization of the VP2 gene of a number of Mexican IPNV isolates was done and the virus's phylogenetic relationships to IPNV reference strains were investigated. The phylogenetic analysis indicated that Mexican IPNV isolates are closely related to strains from the United States and Canada and that all Mexican IPNV isolates belong to genogroup 1. Furthermore, low genetic diversity was found between the Mexican isolates (identity, 95.8–99.8% nucleotides and 95.8–99.6% aminoacids). The result of the analysis of the amino acid residues found at positions 217, 221, and 247 (alanine, threonine, and glutamic acid, respectively) could be associated with virulence, although the expression of virulence factors is more complex and may be influenced by the agent and host factors. The high percentage of identity among the VP2 genes from geographically distant IPNV isolates and the evidence of wide distribution in the country might have been facilitated by carrier trout. This hypothesis is supported by the identification of the amino acid threonine at position 221 in all Mexican isolates, a factor related to the carrier state for IPNV, as reported by other studies. Received July 14, 2013; accepted October 24, 2013 |
author2 |
Consejo Nacional de Ciencia y Tecnología |
format |
Article in Journal/Newspaper |
author |
Salgado‐Miranda, Celene Rojas‐Anaya, Edith García‐Espinosa, Gary Loza‐Rubio, Elizabeth |
spellingShingle |
Salgado‐Miranda, Celene Rojas‐Anaya, Edith García‐Espinosa, Gary Loza‐Rubio, Elizabeth Molecular Characterization of the VP2 Gene of Infectious Pancreatic Necrosis Virus (IPNV) Isolates from Mexico |
author_facet |
Salgado‐Miranda, Celene Rojas‐Anaya, Edith García‐Espinosa, Gary Loza‐Rubio, Elizabeth |
author_sort |
Salgado‐Miranda, Celene |
title |
Molecular Characterization of the VP2 Gene of Infectious Pancreatic Necrosis Virus (IPNV) Isolates from Mexico |
title_short |
Molecular Characterization of the VP2 Gene of Infectious Pancreatic Necrosis Virus (IPNV) Isolates from Mexico |
title_full |
Molecular Characterization of the VP2 Gene of Infectious Pancreatic Necrosis Virus (IPNV) Isolates from Mexico |
title_fullStr |
Molecular Characterization of the VP2 Gene of Infectious Pancreatic Necrosis Virus (IPNV) Isolates from Mexico |
title_full_unstemmed |
Molecular Characterization of the VP2 Gene of Infectious Pancreatic Necrosis Virus (IPNV) Isolates from Mexico |
title_sort |
molecular characterization of the vp2 gene of infectious pancreatic necrosis virus (ipnv) isolates from mexico |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1080/08997659.2013.860060 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/08997659.2013.860060 |
geographic |
Canada Pacific |
geographic_facet |
Canada Pacific |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Aquatic Animal Health volume 26, issue 1, page 43-51 ISSN 0899-7659 1548-8667 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1080/08997659.2013.860060 |
container_title |
Journal of Aquatic Animal Health |
container_volume |
26 |
container_issue |
1 |
container_start_page |
43 |
op_container_end_page |
51 |
_version_ |
1800748303255601152 |