Population Genomic Analysis of Brook Trout in Pennsylvania's Appalachian Region
Abstract Informed conservation of stream fishes requires detailed understanding of the effects of both natural processes and anthropogenic activities on genetic diversity. Brook Trout Salvelinus fontinalis , a salmonid native to eastern North America, typically resides in cold, high‐quality stream e...
Published in: | Transactions of the American Fisheries Society |
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Online Access: | http://dx.doi.org/10.1080/00028487.2017.1285351 https://onlinelibrary.wiley.com/doi/pdf/10.1080/00028487.2017.1285351 https://onlinelibrary.wiley.com/doi/full-xml/10.1080/00028487.2017.1285351 https://afspubs.onlinelibrary.wiley.com/doi/am-pdf/10.1080/00028487.2017.1285351 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/00028487.2017.1285351 |
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crwiley:10.1080/00028487.2017.1285351 2024-06-02T08:03:41+00:00 Population Genomic Analysis of Brook Trout in Pennsylvania's Appalachian Region Buonaccorsi, V. P. Malloy, J. Peterson, M. Brubaker, K. Grant, C. J. Howard Hughes Medical Institute National Science Foundation Howard Hughes Medical Institute National Science Foundation 2017 http://dx.doi.org/10.1080/00028487.2017.1285351 https://onlinelibrary.wiley.com/doi/pdf/10.1080/00028487.2017.1285351 https://onlinelibrary.wiley.com/doi/full-xml/10.1080/00028487.2017.1285351 https://afspubs.onlinelibrary.wiley.com/doi/am-pdf/10.1080/00028487.2017.1285351 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/00028487.2017.1285351 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor Transactions of the American Fisheries Society volume 146, issue 3, page 485-494 ISSN 0002-8487 1548-8659 journal-article 2017 crwiley https://doi.org/10.1080/00028487.2017.1285351 2024-05-03T12:00:33Z Abstract Informed conservation of stream fishes requires detailed understanding of the effects of both natural processes and anthropogenic activities on genetic diversity. Brook Trout Salvelinus fontinalis , a salmonid native to eastern North America, typically resides in cold, high‐quality stream ecosystems. The species has not only faced historical anthropogenic pressures, but also confronts current and future pressures. In a genetic analysis we used a reduced representation sequencing method (ddRADseq) to characterize 63 individuals from 23 streams where Brook Trout are native in the Appalachian region of Pennsylvania. A total of 2,590 loci passed filtering criteria, and 53% displayed significant association with a major stream drainage basin (Susquehanna or Allegheny; mean F ST = 0.085). Mapping of the sequencing reads to the Atlantic Salmon Salmo salar genome revealed no clustering of high interdrainage F ST values to specific genome regions. Evidence for genetic heterogeneity within each drainage basin was also detected. Stepwise regression of observed heterozygosity against geographic and environmental features revealed that drainage basin and effective area of watersheds were significant predictors of observed heterozygosity of Brook Trout within streams. Natural features such as waterfalls and major drainage basin, as well as the effects of dams and acid‐mine drainage have fragmented habitat and shaped genetic diversity within Brook Trout populations in the Appalachian region of Pennsylvania, overall indicating the vulnerability of this species to increased industrialization. Received August 15, 2016; accepted January 17, 2017 Published online March 24, 2017 Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Transactions of the American Fisheries Society 146 3 485 494 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Informed conservation of stream fishes requires detailed understanding of the effects of both natural processes and anthropogenic activities on genetic diversity. Brook Trout Salvelinus fontinalis , a salmonid native to eastern North America, typically resides in cold, high‐quality stream ecosystems. The species has not only faced historical anthropogenic pressures, but also confronts current and future pressures. In a genetic analysis we used a reduced representation sequencing method (ddRADseq) to characterize 63 individuals from 23 streams where Brook Trout are native in the Appalachian region of Pennsylvania. A total of 2,590 loci passed filtering criteria, and 53% displayed significant association with a major stream drainage basin (Susquehanna or Allegheny; mean F ST = 0.085). Mapping of the sequencing reads to the Atlantic Salmon Salmo salar genome revealed no clustering of high interdrainage F ST values to specific genome regions. Evidence for genetic heterogeneity within each drainage basin was also detected. Stepwise regression of observed heterozygosity against geographic and environmental features revealed that drainage basin and effective area of watersheds were significant predictors of observed heterozygosity of Brook Trout within streams. Natural features such as waterfalls and major drainage basin, as well as the effects of dams and acid‐mine drainage have fragmented habitat and shaped genetic diversity within Brook Trout populations in the Appalachian region of Pennsylvania, overall indicating the vulnerability of this species to increased industrialization. Received August 15, 2016; accepted January 17, 2017 Published online March 24, 2017 |
author2 |
Howard Hughes Medical Institute National Science Foundation Howard Hughes Medical Institute National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Buonaccorsi, V. P. Malloy, J. Peterson, M. Brubaker, K. Grant, C. J. |
spellingShingle |
Buonaccorsi, V. P. Malloy, J. Peterson, M. Brubaker, K. Grant, C. J. Population Genomic Analysis of Brook Trout in Pennsylvania's Appalachian Region |
author_facet |
Buonaccorsi, V. P. Malloy, J. Peterson, M. Brubaker, K. Grant, C. J. |
author_sort |
Buonaccorsi, V. P. |
title |
Population Genomic Analysis of Brook Trout in Pennsylvania's Appalachian Region |
title_short |
Population Genomic Analysis of Brook Trout in Pennsylvania's Appalachian Region |
title_full |
Population Genomic Analysis of Brook Trout in Pennsylvania's Appalachian Region |
title_fullStr |
Population Genomic Analysis of Brook Trout in Pennsylvania's Appalachian Region |
title_full_unstemmed |
Population Genomic Analysis of Brook Trout in Pennsylvania's Appalachian Region |
title_sort |
population genomic analysis of brook trout in pennsylvania's appalachian region |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1080/00028487.2017.1285351 https://onlinelibrary.wiley.com/doi/pdf/10.1080/00028487.2017.1285351 https://onlinelibrary.wiley.com/doi/full-xml/10.1080/00028487.2017.1285351 https://afspubs.onlinelibrary.wiley.com/doi/am-pdf/10.1080/00028487.2017.1285351 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1080/00028487.2017.1285351 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Transactions of the American Fisheries Society volume 146, issue 3, page 485-494 ISSN 0002-8487 1548-8659 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1080/00028487.2017.1285351 |
container_title |
Transactions of the American Fisheries Society |
container_volume |
146 |
container_issue |
3 |
container_start_page |
485 |
op_container_end_page |
494 |
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1800748290962096128 |