CHARACTERIZATION OF HaRNAV, A SINGLE‐STRANDED RNA VIRUS CAUSING LYSIS OF HETEROSIGMA AKASHIWO (RAPHIDOPHYCEAE) 1
HaRNAV, a novel virus that infects the toxic bloom‐forming alga Heterosigma akashiwo (Hada) Hada ex Hada et Chihara, was characterized based on morphology, pathology, nucleic acid type, structural proteins, and the range of host strains that it infects. HaRNAV is a 25‐nm single‐stranded RNA (ssRNA)...
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crwiley:10.1046/j.1529-8817.2003.01162.x 2024-06-23T07:55:40+00:00 CHARACTERIZATION OF HaRNAV, A SINGLE‐STRANDED RNA VIRUS CAUSING LYSIS OF HETEROSIGMA AKASHIWO (RAPHIDOPHYCEAE) 1 Tai, Vera Lawrence, Janice E. Lang, Andrew S. Chan, Amy M. Culley, Alexander I. Suttle, Curtis A. 2003 http://dx.doi.org/10.1046/j.1529-8817.2003.01162.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1529-8817.2003.01162.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1529-8817.2003.01162.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 39, issue 2, page 343-352 ISSN 0022-3646 1529-8817 journal-article 2003 crwiley https://doi.org/10.1046/j.1529-8817.2003.01162.x 2024-06-13T04:24:03Z HaRNAV, a novel virus that infects the toxic bloom‐forming alga Heterosigma akashiwo (Hada) Hada ex Hada et Chihara, was characterized based on morphology, pathology, nucleic acid type, structural proteins, and the range of host strains that it infects. HaRNAV is a 25‐nm single‐stranded RNA (ssRNA) virus with a genome size of approximately 9100 nucleotides. This is the first report of an ssRNA virus that causes lysis of a phytoplankton species. The virus particle is sensitive to chloroform and contains at least five structural proteins ranging in apparent size from 24 to 34 kDa. HaRNAV infection causes swelling of the endoplasmic reticulum and progeny virus particles assemble in the cytoplasm of the host, frequently in crystalline arrays. The infectivity of HaRNAV was tested against 15 strains of H. akashiwo isolated from Japanese waters, the Northeast Pacific, and the Northwest Atlantic. HaRNAV caused lysis of three strains from the Northeast Pacific and two strains from Japan but none from the Northwest Atlantic. The characterization of HaRNAV demonstrates that HaRNAV is a novel type of phytoplankton virus but has some similarities with plant viruses belonging to the Sequiviridae and to other known ssRNA viruses. Further genomic analysis, however, is necessary to determine any phylogenetic relationships. The discovery of HaRNAV emphasizes the diversity of H. akashiwo viral pathogens and, more importantly, algal–virus pathogens and the complexity of virus–host interactions in the environment. Article in Journal/Newspaper Northwest Atlantic Wiley Online Library Pacific Journal of Phycology 39 2 343 352 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
HaRNAV, a novel virus that infects the toxic bloom‐forming alga Heterosigma akashiwo (Hada) Hada ex Hada et Chihara, was characterized based on morphology, pathology, nucleic acid type, structural proteins, and the range of host strains that it infects. HaRNAV is a 25‐nm single‐stranded RNA (ssRNA) virus with a genome size of approximately 9100 nucleotides. This is the first report of an ssRNA virus that causes lysis of a phytoplankton species. The virus particle is sensitive to chloroform and contains at least five structural proteins ranging in apparent size from 24 to 34 kDa. HaRNAV infection causes swelling of the endoplasmic reticulum and progeny virus particles assemble in the cytoplasm of the host, frequently in crystalline arrays. The infectivity of HaRNAV was tested against 15 strains of H. akashiwo isolated from Japanese waters, the Northeast Pacific, and the Northwest Atlantic. HaRNAV caused lysis of three strains from the Northeast Pacific and two strains from Japan but none from the Northwest Atlantic. The characterization of HaRNAV demonstrates that HaRNAV is a novel type of phytoplankton virus but has some similarities with plant viruses belonging to the Sequiviridae and to other known ssRNA viruses. Further genomic analysis, however, is necessary to determine any phylogenetic relationships. The discovery of HaRNAV emphasizes the diversity of H. akashiwo viral pathogens and, more importantly, algal–virus pathogens and the complexity of virus–host interactions in the environment. |
format |
Article in Journal/Newspaper |
author |
Tai, Vera Lawrence, Janice E. Lang, Andrew S. Chan, Amy M. Culley, Alexander I. Suttle, Curtis A. |
spellingShingle |
Tai, Vera Lawrence, Janice E. Lang, Andrew S. Chan, Amy M. Culley, Alexander I. Suttle, Curtis A. CHARACTERIZATION OF HaRNAV, A SINGLE‐STRANDED RNA VIRUS CAUSING LYSIS OF HETEROSIGMA AKASHIWO (RAPHIDOPHYCEAE) 1 |
author_facet |
Tai, Vera Lawrence, Janice E. Lang, Andrew S. Chan, Amy M. Culley, Alexander I. Suttle, Curtis A. |
author_sort |
Tai, Vera |
title |
CHARACTERIZATION OF HaRNAV, A SINGLE‐STRANDED RNA VIRUS CAUSING LYSIS OF HETEROSIGMA AKASHIWO (RAPHIDOPHYCEAE) 1 |
title_short |
CHARACTERIZATION OF HaRNAV, A SINGLE‐STRANDED RNA VIRUS CAUSING LYSIS OF HETEROSIGMA AKASHIWO (RAPHIDOPHYCEAE) 1 |
title_full |
CHARACTERIZATION OF HaRNAV, A SINGLE‐STRANDED RNA VIRUS CAUSING LYSIS OF HETEROSIGMA AKASHIWO (RAPHIDOPHYCEAE) 1 |
title_fullStr |
CHARACTERIZATION OF HaRNAV, A SINGLE‐STRANDED RNA VIRUS CAUSING LYSIS OF HETEROSIGMA AKASHIWO (RAPHIDOPHYCEAE) 1 |
title_full_unstemmed |
CHARACTERIZATION OF HaRNAV, A SINGLE‐STRANDED RNA VIRUS CAUSING LYSIS OF HETEROSIGMA AKASHIWO (RAPHIDOPHYCEAE) 1 |
title_sort |
characterization of harnav, a single‐stranded rna virus causing lysis of heterosigma akashiwo (raphidophyceae) 1 |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1046/j.1529-8817.2003.01162.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1529-8817.2003.01162.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1529-8817.2003.01162.x |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_source |
Journal of Phycology volume 39, issue 2, page 343-352 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1529-8817.2003.01162.x |
container_title |
Journal of Phycology |
container_volume |
39 |
container_issue |
2 |
container_start_page |
343 |
op_container_end_page |
352 |
_version_ |
1802648335230697472 |