A SYSTEMATIC STUDY OF GIGAR‐TINACEAE FROM PACIFIC NORTH AMERICA BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE
Greater than 50 species of Gigartinaceae have been described from Pacific North America, about half of which are currently recognized. Although the family is treated extensively in the taxonomic literature, many of the species are still confused and a comprehensive revision is required. We sequenced...
Published in: | Journal of Phycology |
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Online Access: | http://dx.doi.org/10.1046/j.1529-8817.1999.00001-97.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1529-8817.1999.00001-97.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1529-8817.1999.00001-97.x |
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crwiley:10.1046/j.1529-8817.1999.00001-97.x 2024-06-02T08:14:49+00:00 A SYSTEMATIC STUDY OF GIGAR‐TINACEAE FROM PACIFIC NORTH AMERICA BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE Hughey, J.R. Hommersand, M.H. 2000 http://dx.doi.org/10.1046/j.1529-8817.1999.00001-97.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1529-8817.1999.00001-97.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1529-8817.1999.00001-97.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 36, issue s3, page 32-32 ISSN 0022-3646 1529-8817 journal-article 2000 crwiley https://doi.org/10.1046/j.1529-8817.1999.00001-97.x 2024-05-03T10:43:48Z Greater than 50 species of Gigartinaceae have been described from Pacific North America, about half of which are currently recognized. Although the family is treated extensively in the taxonomic literature, many of the species are still confused and a comprehensive revision is required. We sequenced the rbc L (RuBisCO) gene and ITS (Internal Transcribed Spacer) 1, 2, and 5.8S regions from a large number of recent collections and identified a discrete of number data sets. These were analysed in comparison with the morphological evidence for each of the taxa. Uncertain of the possibility that our operational taxonomic units may not correspond to the types, we developed a protocol for isolating PCR‐friendly DNA from herbarium specimens, some reaching back as far as 1670. The DNA profiles of types and historically important specimens were compared to those for recently collected silica gel‐dried and formalin‐fixed material and assigned correct names. Species studied ranged from Alaska to Mexico and the Gulf of California and were compared to outgroup taxa from Pacific South America and the Southern Ocean. Particular attention was paid to variations in morphology as they relate to habitat with emphasis on the presence or absence of different morphological forms among sympatric and allopatric populations. We recognize 10 species in Chondracanthus (including one new combination and one new species) and 16 species in Mazzaella (including two new combinations and two new species). Finally, we tested a phylogenetic hypothesis inferred for the Gigartinaceae from rbc L sequences for congruence with one generated from ITS sequences. Article in Journal/Newspaper Southern Ocean Alaska Wiley Online Library Pacific Southern Ocean Journal of Phycology 36 s3 32 32 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Greater than 50 species of Gigartinaceae have been described from Pacific North America, about half of which are currently recognized. Although the family is treated extensively in the taxonomic literature, many of the species are still confused and a comprehensive revision is required. We sequenced the rbc L (RuBisCO) gene and ITS (Internal Transcribed Spacer) 1, 2, and 5.8S regions from a large number of recent collections and identified a discrete of number data sets. These were analysed in comparison with the morphological evidence for each of the taxa. Uncertain of the possibility that our operational taxonomic units may not correspond to the types, we developed a protocol for isolating PCR‐friendly DNA from herbarium specimens, some reaching back as far as 1670. The DNA profiles of types and historically important specimens were compared to those for recently collected silica gel‐dried and formalin‐fixed material and assigned correct names. Species studied ranged from Alaska to Mexico and the Gulf of California and were compared to outgroup taxa from Pacific South America and the Southern Ocean. Particular attention was paid to variations in morphology as they relate to habitat with emphasis on the presence or absence of different morphological forms among sympatric and allopatric populations. We recognize 10 species in Chondracanthus (including one new combination and one new species) and 16 species in Mazzaella (including two new combinations and two new species). Finally, we tested a phylogenetic hypothesis inferred for the Gigartinaceae from rbc L sequences for congruence with one generated from ITS sequences. |
format |
Article in Journal/Newspaper |
author |
Hughey, J.R. Hommersand, M.H. |
spellingShingle |
Hughey, J.R. Hommersand, M.H. A SYSTEMATIC STUDY OF GIGAR‐TINACEAE FROM PACIFIC NORTH AMERICA BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE |
author_facet |
Hughey, J.R. Hommersand, M.H. |
author_sort |
Hughey, J.R. |
title |
A SYSTEMATIC STUDY OF GIGAR‐TINACEAE FROM PACIFIC NORTH AMERICA BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE |
title_short |
A SYSTEMATIC STUDY OF GIGAR‐TINACEAE FROM PACIFIC NORTH AMERICA BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE |
title_full |
A SYSTEMATIC STUDY OF GIGAR‐TINACEAE FROM PACIFIC NORTH AMERICA BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE |
title_fullStr |
A SYSTEMATIC STUDY OF GIGAR‐TINACEAE FROM PACIFIC NORTH AMERICA BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE |
title_full_unstemmed |
A SYSTEMATIC STUDY OF GIGAR‐TINACEAE FROM PACIFIC NORTH AMERICA BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE |
title_sort |
systematic study of gigar‐tinaceae from pacific north america based on molecular and morphological evidence |
publisher |
Wiley |
publishDate |
2000 |
url |
http://dx.doi.org/10.1046/j.1529-8817.1999.00001-97.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1529-8817.1999.00001-97.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1529-8817.1999.00001-97.x |
geographic |
Pacific Southern Ocean |
geographic_facet |
Pacific Southern Ocean |
genre |
Southern Ocean Alaska |
genre_facet |
Southern Ocean Alaska |
op_source |
Journal of Phycology volume 36, issue s3, page 32-32 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1529-8817.1999.00001-97.x |
container_title |
Journal of Phycology |
container_volume |
36 |
container_issue |
s3 |
container_start_page |
32 |
op_container_end_page |
32 |
_version_ |
1800738791159234560 |