Characterization of polymorphic microsatellite DNA markers in the ruff ( Philomachus pugnax)

• Published in: Molecular Ecology Notes
• Main Authors: Thuman, Katherine A., Widemo, Fredrik, Piertney, Stuart B.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2002
• Subjects: Philomachus pugnax; Ruff
• Online Access: http://dx.doi.org/10.1046/j.1471-8286.2002.00221.x-i2; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-8286.2002.00221.x-i2; https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-8286.2002.00221.x-i2

Abstract Nine polymorphic microsatellite markers were isolated and characterized from the lek‐breeding ruff ( Philomachus pugnax ) using an enrichment cloning procedure. The number of alleles resolved per locus ranged from five to 56, and observed heterozygosity ranged from 0.311 to 0.857 in a sample of 319 ruffs from Gotland, Sweden. Two loci were located on the Z chromosome, rendering all females hemizygotes. These markers are effective in the assignment of parentage and determining male mating success.