Characterization of polymorphic microsatellite DNA markers in the ruff ( Philomachus pugnax)
Abstract Nine polymorphic microsatellite markers were isolated and characterized from the lek‐breeding ruff ( Philomachus pugnax ) using an enrichment cloning procedure. The number of alleles resolved per locus ranged from five to 56, and observed heterozygosity ranged from 0.311 to 0.857 in a sampl...
| Published in: | Molecular Ecology Notes |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2002
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1046/j.1471-8286.2002.00221.x-i2 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-8286.2002.00221.x-i2 https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-8286.2002.00221.x-i2 |
| Summary: | Abstract Nine polymorphic microsatellite markers were isolated and characterized from the lek‐breeding ruff ( Philomachus pugnax ) using an enrichment cloning procedure. The number of alleles resolved per locus ranged from five to 56, and observed heterozygosity ranged from 0.311 to 0.857 in a sample of 319 ruffs from Gotland, Sweden. Two loci were located on the Z chromosome, rendering all females hemizygotes. These markers are effective in the assignment of parentage and determining male mating success. |
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