Dinucleotide microsatellite markers from the Antarctic seals and their use in other Pinnipeds

• Published in: Molecular Ecology Notes
• Main Authors: Davis, C. S., Gelatt, T. S., Siniff, D., Strobeck, C.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2002
• Subjects: Antarc*; Antarctic; Bird Island; Crabeater Seal; Crabeater Seals; Hydrurga leptonyx; Leopard Seal; Leopard Seals; Lobodon carcinophagus; McMurdo Sound; Ross Sea; Ross Seal; Weddell Seal; Weddell Seals
• Online Access: http://dx.doi.org/10.1046/j.1471-8286.2002.00187.x-i2; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-8286.2002.00187.x-i2; https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-8286.2002.00187.x-i2

Abstract Twenty‐four microsatellite loci were isolated from three species of Antarctic seals (Subfamily Monachinae, Tribe Lobodontini). Eleven loci were cloned from Weddell seal, Leptonychotes weddellii , seven from leopard seal, Hydrurga leptonyx , and six from crabeater seal, Lobodon carcinophagus . Variability was assessed in Weddell seals collected in McMurdo Sound, leopard seals from Bird Island, South Georgia, and crabeater seals sampled in the eastern Ross Sea. All loci were variable in the three species used for cloning and 22 of these loci amplified variable products in the Ross seal, Ommatophoca rossii . Cross‐species amplification was largely successful, with an average of 19 loci amplifying products in other phocids.