Nondestructive DNA extraction from sperm whale teeth and scrimshaw
Abstract Sperm whales ( Physeter macrocephalus ), have been hunted throughout their global distribution. As a result, sizeable collections of sperm whale teeth and scrimshaw are held in museum and private collections. Access to these specimens for genetic analysis is limited due to the tendency of D...
Published in: | Molecular Ecology Notes |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2001
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1046/j.1471-8278.2001.00027.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-8278.2001.00027.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-8278.2001.00027.x |
id |
crwiley:10.1046/j.1471-8278.2001.00027.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1046/j.1471-8278.2001.00027.x 2024-06-02T08:13:16+00:00 Nondestructive DNA extraction from sperm whale teeth and scrimshaw Pichler, F. B. Dalebout, M. L. Baker, C. S. 2001 http://dx.doi.org/10.1046/j.1471-8278.2001.00027.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-8278.2001.00027.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-8278.2001.00027.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Notes volume 1, issue 1-2, page 106-109 ISSN 1471-8278 1471-8286 journal-article 2001 crwiley https://doi.org/10.1046/j.1471-8278.2001.00027.x 2024-05-03T11:16:10Z Abstract Sperm whales ( Physeter macrocephalus ), have been hunted throughout their global distribution. As a result, sizeable collections of sperm whale teeth and scrimshaw are held in museum and private collections. Access to these specimens for genetic analysis is limited due to the tendency of DNA extraction techniques to destructively sample such specimens. Here we demonstrate that small amounts of bone powder, collected by drilling inside the root cavity, can yield sufficient DNA for sequencing without adversely affecting the specimen's appearance. We anticipate that owners and curators of sperm whale teeth will be more amenable to genetic analysis using this extraction method. Article in Journal/Newspaper Physeter macrocephalus Sperm whale Wiley Online Library Molecular Ecology Notes 1 1-2 106 109 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Sperm whales ( Physeter macrocephalus ), have been hunted throughout their global distribution. As a result, sizeable collections of sperm whale teeth and scrimshaw are held in museum and private collections. Access to these specimens for genetic analysis is limited due to the tendency of DNA extraction techniques to destructively sample such specimens. Here we demonstrate that small amounts of bone powder, collected by drilling inside the root cavity, can yield sufficient DNA for sequencing without adversely affecting the specimen's appearance. We anticipate that owners and curators of sperm whale teeth will be more amenable to genetic analysis using this extraction method. |
format |
Article in Journal/Newspaper |
author |
Pichler, F. B. Dalebout, M. L. Baker, C. S. |
spellingShingle |
Pichler, F. B. Dalebout, M. L. Baker, C. S. Nondestructive DNA extraction from sperm whale teeth and scrimshaw |
author_facet |
Pichler, F. B. Dalebout, M. L. Baker, C. S. |
author_sort |
Pichler, F. B. |
title |
Nondestructive DNA extraction from sperm whale teeth and scrimshaw |
title_short |
Nondestructive DNA extraction from sperm whale teeth and scrimshaw |
title_full |
Nondestructive DNA extraction from sperm whale teeth and scrimshaw |
title_fullStr |
Nondestructive DNA extraction from sperm whale teeth and scrimshaw |
title_full_unstemmed |
Nondestructive DNA extraction from sperm whale teeth and scrimshaw |
title_sort |
nondestructive dna extraction from sperm whale teeth and scrimshaw |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1046/j.1471-8278.2001.00027.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-8278.2001.00027.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-8278.2001.00027.x |
genre |
Physeter macrocephalus Sperm whale |
genre_facet |
Physeter macrocephalus Sperm whale |
op_source |
Molecular Ecology Notes volume 1, issue 1-2, page 106-109 ISSN 1471-8278 1471-8286 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1471-8278.2001.00027.x |
container_title |
Molecular Ecology Notes |
container_volume |
1 |
container_issue |
1-2 |
container_start_page |
106 |
op_container_end_page |
109 |
_version_ |
1800736713536962560 |