Tyrosine Hydroxylase in the European Eel ( Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis
Abstract: We report the isolation of a full‐length eel tyrosine hydroxylase (TH) cDNA that is characterized by a long 3′ untranslated region and by a diversity restricted to the 3′ end owing to the differential use of three polyadenylation signals. The longest eel TH mRNA was distinctive in the pres...
Published in: | Journal of Neurochemistry |
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crwiley:10.1046/j.1471-4159.1998.71020460.x 2024-06-02T07:55:12+00:00 Tyrosine Hydroxylase in the European Eel ( Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis Boularand, Sylviane Biguet, Nicole Faucon Vidal, Bernadette Veron, Michèle Mallet, Jacques Vincent, Jean‐Didier Dufour, Sylvie Vernier, Philippe 1998 http://dx.doi.org/10.1046/j.1471-4159.1998.71020460.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-4159.1998.71020460.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-4159.1998.71020460.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Neurochemistry volume 71, issue 2, page 460-470 ISSN 0022-3042 1471-4159 journal-article 1998 crwiley https://doi.org/10.1046/j.1471-4159.1998.71020460.x 2024-05-03T11:34:45Z Abstract: We report the isolation of a full‐length eel tyrosine hydroxylase (TH) cDNA that is characterized by a long 3′ untranslated region and by a diversity restricted to the 3′ end owing to the differential use of three polyadenylation signals. The longest eel TH mRNA was distinctive in the presence of four pentameric elements (AUUUA) in the AU‐rich 3′ noncoding region. Such a diversity could provide the basis of posttranscriptional or translational regulation of eel TH gene expression. Comparison of the eel TH sequence with those of other aromatic amino acid hydroxylases (TH, tryptophan hydroxylase, and phenylalanine hydroxylase) and phylogenetic analysis confirmed that the N‐terminal regulatory domain is highly divergent, contrasting with the conservation of the catalytic core of the enzyme. Molecular phylogenies including the available sequences of the three hydroxylase genes suggested that the duplication of their common ancestor occurred before the emergence of arthropods. The regional expression of the eel TH mRNA was studied by semiquantitative PCR, northern blots, and in situ hybridization and compared with the immunocytochemical localization of TH protein. The data showed that TH mRNA is mostly expressed in the olfactory and hypothalamic areas, whereas sparse TH‐expressing cell bodies are present in the telencephalic region and brainstem. No labeling was detected in the mesencephalic area, in striking contrast with that found in amphibians and amniotes. Article in Journal/Newspaper Anguilla anguilla Wiley Online Library Journal of Neurochemistry 71 2 460 470 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract: We report the isolation of a full‐length eel tyrosine hydroxylase (TH) cDNA that is characterized by a long 3′ untranslated region and by a diversity restricted to the 3′ end owing to the differential use of three polyadenylation signals. The longest eel TH mRNA was distinctive in the presence of four pentameric elements (AUUUA) in the AU‐rich 3′ noncoding region. Such a diversity could provide the basis of posttranscriptional or translational regulation of eel TH gene expression. Comparison of the eel TH sequence with those of other aromatic amino acid hydroxylases (TH, tryptophan hydroxylase, and phenylalanine hydroxylase) and phylogenetic analysis confirmed that the N‐terminal regulatory domain is highly divergent, contrasting with the conservation of the catalytic core of the enzyme. Molecular phylogenies including the available sequences of the three hydroxylase genes suggested that the duplication of their common ancestor occurred before the emergence of arthropods. The regional expression of the eel TH mRNA was studied by semiquantitative PCR, northern blots, and in situ hybridization and compared with the immunocytochemical localization of TH protein. The data showed that TH mRNA is mostly expressed in the olfactory and hypothalamic areas, whereas sparse TH‐expressing cell bodies are present in the telencephalic region and brainstem. No labeling was detected in the mesencephalic area, in striking contrast with that found in amphibians and amniotes. |
format |
Article in Journal/Newspaper |
author |
Boularand, Sylviane Biguet, Nicole Faucon Vidal, Bernadette Veron, Michèle Mallet, Jacques Vincent, Jean‐Didier Dufour, Sylvie Vernier, Philippe |
spellingShingle |
Boularand, Sylviane Biguet, Nicole Faucon Vidal, Bernadette Veron, Michèle Mallet, Jacques Vincent, Jean‐Didier Dufour, Sylvie Vernier, Philippe Tyrosine Hydroxylase in the European Eel ( Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
author_facet |
Boularand, Sylviane Biguet, Nicole Faucon Vidal, Bernadette Veron, Michèle Mallet, Jacques Vincent, Jean‐Didier Dufour, Sylvie Vernier, Philippe |
author_sort |
Boularand, Sylviane |
title |
Tyrosine Hydroxylase in the European Eel ( Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_short |
Tyrosine Hydroxylase in the European Eel ( Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_full |
Tyrosine Hydroxylase in the European Eel ( Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_fullStr |
Tyrosine Hydroxylase in the European Eel ( Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_full_unstemmed |
Tyrosine Hydroxylase in the European Eel ( Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_sort |
tyrosine hydroxylase in the european eel ( anguilla anguilla): cdna cloning, brain distribution, and phylogenetic analysis |
publisher |
Wiley |
publishDate |
1998 |
url |
http://dx.doi.org/10.1046/j.1471-4159.1998.71020460.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-4159.1998.71020460.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-4159.1998.71020460.x |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Journal of Neurochemistry volume 71, issue 2, page 460-470 ISSN 0022-3042 1471-4159 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1471-4159.1998.71020460.x |
container_title |
Journal of Neurochemistry |
container_volume |
71 |
container_issue |
2 |
container_start_page |
460 |
op_container_end_page |
470 |
_version_ |
1800746850163097600 |