Isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plant Ranunculus adoneus
Summary Dark‐septate endophytic (DSE) fungi are ubiquitous in the roots of Arctic and alpine plants, yet very little is known about their phylogenetic identities or effects on their host plants. Several such fungi were isolated from the alpine snowbed plant Ranunculus adoneus in the Front Range of C...
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crwiley:10.1046/j.1469-8137.2001.00132.x 2024-06-02T08:02:23+00:00 Isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plant Ranunculus adoneus Schadt, Christopher W. Mullen, Renée B. Schmidt, Steven K. 2001 http://dx.doi.org/10.1046/j.1469-8137.2001.00132.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1469-8137.2001.00132.x https://nph.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1469-8137.2001.00132.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor New Phytologist volume 150, issue 3, page 747-755 ISSN 0028-646X 1469-8137 journal-article 2001 crwiley https://doi.org/10.1046/j.1469-8137.2001.00132.x 2024-05-03T11:07:30Z Summary Dark‐septate endophytic (DSE) fungi are ubiquitous in the roots of Arctic and alpine plants, yet very little is known about their phylogenetic identities or effects on their host plants. Several such fungi were isolated from the alpine snowbed plant Ranunculus adoneus in the Front Range of Colorado, USA; one isolate was chosen for detailed study. The ability of this isolate to re‐colonize plant roots in pot cultures was assessed, and phylogenetic analyses were performed using small‐subunit (SSU), 5.8S and internal transcribed spacer (ITS) 2 ribosomal DNA sequences. This isolate had the ability to produce root endophytic structures in pot cultures similar to those reported from other sources and observed in R. adoneus roots. SSU phylogenetic analyses showed this isolate to be related to a clade within the Euascomycetes containing the Leotiales and Erysiphales. In addition, SSU and 5.8S–ITS2 sequences showed high phylogenetic similarity to a variety of isolates reported from other plants of diverse geographical origins. Although most of these isolates remain unidentified, one closely related isolate was the anamorphic taxon Phialophora gregata . The results suggest that this DSE isolate might belong to the fairly closely related group of plant endophytes that have varied effects on the plants that they inhabit. Article in Journal/Newspaper Arctic Wiley Online Library Arctic New Phytologist 150 3 747 755 |
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Wiley Online Library |
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English |
description |
Summary Dark‐septate endophytic (DSE) fungi are ubiquitous in the roots of Arctic and alpine plants, yet very little is known about their phylogenetic identities or effects on their host plants. Several such fungi were isolated from the alpine snowbed plant Ranunculus adoneus in the Front Range of Colorado, USA; one isolate was chosen for detailed study. The ability of this isolate to re‐colonize plant roots in pot cultures was assessed, and phylogenetic analyses were performed using small‐subunit (SSU), 5.8S and internal transcribed spacer (ITS) 2 ribosomal DNA sequences. This isolate had the ability to produce root endophytic structures in pot cultures similar to those reported from other sources and observed in R. adoneus roots. SSU phylogenetic analyses showed this isolate to be related to a clade within the Euascomycetes containing the Leotiales and Erysiphales. In addition, SSU and 5.8S–ITS2 sequences showed high phylogenetic similarity to a variety of isolates reported from other plants of diverse geographical origins. Although most of these isolates remain unidentified, one closely related isolate was the anamorphic taxon Phialophora gregata . The results suggest that this DSE isolate might belong to the fairly closely related group of plant endophytes that have varied effects on the plants that they inhabit. |
format |
Article in Journal/Newspaper |
author |
Schadt, Christopher W. Mullen, Renée B. Schmidt, Steven K. |
spellingShingle |
Schadt, Christopher W. Mullen, Renée B. Schmidt, Steven K. Isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plant Ranunculus adoneus |
author_facet |
Schadt, Christopher W. Mullen, Renée B. Schmidt, Steven K. |
author_sort |
Schadt, Christopher W. |
title |
Isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plant Ranunculus adoneus |
title_short |
Isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plant Ranunculus adoneus |
title_full |
Isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plant Ranunculus adoneus |
title_fullStr |
Isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plant Ranunculus adoneus |
title_full_unstemmed |
Isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plant Ranunculus adoneus |
title_sort |
isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plant ranunculus adoneus |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1046/j.1469-8137.2001.00132.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1469-8137.2001.00132.x https://nph.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1469-8137.2001.00132.x |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
New Phytologist volume 150, issue 3, page 747-755 ISSN 0028-646X 1469-8137 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1469-8137.2001.00132.x |
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New Phytologist |
container_volume |
150 |
container_issue |
3 |
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747 |
op_container_end_page |
755 |
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1800746885226430464 |