Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice
Lake Vostok, the largest subglacial lake in Antarctica, is separated from the surface by ≈ 4 km of glacial ice. It has been isolated from direct surface input for at least 420 000 years, and the possibility of a novel environment and ecosystem therefore exists. Lake Vostok water has not been sampled...
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crwiley:10.1046/j.1462-2920.2001.00226.x 2024-09-15T17:47:54+00:00 Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice Christner, Brent C. Mosley‐Thompson, Ellen Thompson, Lonnie G. Reeve, John N. 2001 http://dx.doi.org/10.1046/j.1462-2920.2001.00226.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1462-2920.2001.00226.x http://onlinelibrary.wiley.com/wol1/doi/10.1046/j.1462-2920.2001.00226.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 3, issue 9, page 570-577 ISSN 1462-2912 1462-2920 journal-article 2001 crwiley https://doi.org/10.1046/j.1462-2920.2001.00226.x 2024-08-22T04:16:59Z Lake Vostok, the largest subglacial lake in Antarctica, is separated from the surface by ≈ 4 km of glacial ice. It has been isolated from direct surface input for at least 420 000 years, and the possibility of a novel environment and ecosystem therefore exists. Lake Vostok water has not been sampled, but an ice core has been recovered that extends into the ice accreted below glacial ice by freezing of Lake Vostok water. Here, we report the recovery of bacterial isolates belonging to the Brachybacteria , Methylobacterium , Paenibacillus and Sphingomonas lineages from a sample of melt water from this accretion ice that originated 3593 m below the surface. We have also amplified small‐subunit ribosomal RNA‐encoding DNA molecules (16S rDNAs) directly from this melt water that originated from α‐ and β‐proteobacteria, low‐ and high‐G+C Gram‐positive bacteria and a member of the Cytophaga / Flavobacterium / Bacteroides lineage. Article in Journal/Newspaper Antarc* Antarctica ice core Wiley Online Library Environmental Microbiology 3 9 570 577 |
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Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Lake Vostok, the largest subglacial lake in Antarctica, is separated from the surface by ≈ 4 km of glacial ice. It has been isolated from direct surface input for at least 420 000 years, and the possibility of a novel environment and ecosystem therefore exists. Lake Vostok water has not been sampled, but an ice core has been recovered that extends into the ice accreted below glacial ice by freezing of Lake Vostok water. Here, we report the recovery of bacterial isolates belonging to the Brachybacteria , Methylobacterium , Paenibacillus and Sphingomonas lineages from a sample of melt water from this accretion ice that originated 3593 m below the surface. We have also amplified small‐subunit ribosomal RNA‐encoding DNA molecules (16S rDNAs) directly from this melt water that originated from α‐ and β‐proteobacteria, low‐ and high‐G+C Gram‐positive bacteria and a member of the Cytophaga / Flavobacterium / Bacteroides lineage. |
format |
Article in Journal/Newspaper |
author |
Christner, Brent C. Mosley‐Thompson, Ellen Thompson, Lonnie G. Reeve, John N. |
spellingShingle |
Christner, Brent C. Mosley‐Thompson, Ellen Thompson, Lonnie G. Reeve, John N. Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
author_facet |
Christner, Brent C. Mosley‐Thompson, Ellen Thompson, Lonnie G. Reeve, John N. |
author_sort |
Christner, Brent C. |
title |
Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_short |
Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_full |
Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_fullStr |
Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_full_unstemmed |
Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice |
title_sort |
isolation of bacteria and 16s rdnas from lake vostok accretion ice |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1046/j.1462-2920.2001.00226.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1462-2920.2001.00226.x http://onlinelibrary.wiley.com/wol1/doi/10.1046/j.1462-2920.2001.00226.x/fullpdf |
genre |
Antarc* Antarctica ice core |
genre_facet |
Antarc* Antarctica ice core |
op_source |
Environmental Microbiology volume 3, issue 9, page 570-577 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1462-2920.2001.00226.x |
container_title |
Environmental Microbiology |
container_volume |
3 |
container_issue |
9 |
container_start_page |
570 |
op_container_end_page |
577 |
_version_ |
1810497594204356608 |