Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda: Loliginidae)
Sexually mature female squid Loligo pealei harbour dense bacterial communities in their accessory nidamental glands (ANGs) and in their egg capsules (ECs). This study describes a molecular approach using the 16S rRNA gene (rDNA) to identify bacterial populations within the ANG and the ECs of the Nor...
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Online Access: | http://dx.doi.org/10.1046/j.1462-2920.2001.00172.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1462-2920.2001.00172.x http://onlinelibrary.wiley.com/wol1/doi/10.1046/j.1462-2920.2001.00172.x/fullpdf |
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crwiley:10.1046/j.1462-2920.2001.00172.x 2024-09-15T18:23:25+00:00 Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda: Loliginidae) Barbieri, Elena Paster, Bruce J. Hughes, Deborah Zurek, Ludek Moser, Duane P. Teske, Andreas Sogin, Mitchell L. 2001 http://dx.doi.org/10.1046/j.1462-2920.2001.00172.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1462-2920.2001.00172.x http://onlinelibrary.wiley.com/wol1/doi/10.1046/j.1462-2920.2001.00172.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 3, issue 3, page 151-167 ISSN 1462-2912 1462-2920 journal-article 2001 crwiley https://doi.org/10.1046/j.1462-2920.2001.00172.x 2024-08-27T04:30:07Z Sexually mature female squid Loligo pealei harbour dense bacterial communities in their accessory nidamental glands (ANGs) and in their egg capsules (ECs). This study describes a molecular approach using the 16S rRNA gene (rDNA) to identify bacterial populations within the ANG and the ECs of the North Atlantic squid species L. pealei . Fluorescent in situ hybridization (FISH) and 16S rDNA analysis showed that predominantly alpha‐ and, to a lesser extent, gamma‐proteobacteria were the predominant components of the ANG and EC bacterial communities. Sequencing results showed the presence of alpha‐proteobacterial populations affiliated with the Roseobacter group and additional deep‐branching alphaproteobacterial lineages. In contrast, isolates from the ANG and ECs contained only a few alpha‐proteobacteria of the Roseobacter group compared with several gamma‐proteobacterial isolates, mostly Shewanella and Pseudoalteromonas species. Most of the ANG‐associated bacterial populations were also found within the ECs of L. pealei . The molecular approach allowed the visualization of alpha‐proteobacteria as major constituents of a bacterial symbiosis within the reproductive system of the Loliginidae. Article in Journal/Newspaper North Atlantic Wiley Online Library Environmental Microbiology 3 3 151 167 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Sexually mature female squid Loligo pealei harbour dense bacterial communities in their accessory nidamental glands (ANGs) and in their egg capsules (ECs). This study describes a molecular approach using the 16S rRNA gene (rDNA) to identify bacterial populations within the ANG and the ECs of the North Atlantic squid species L. pealei . Fluorescent in situ hybridization (FISH) and 16S rDNA analysis showed that predominantly alpha‐ and, to a lesser extent, gamma‐proteobacteria were the predominant components of the ANG and EC bacterial communities. Sequencing results showed the presence of alpha‐proteobacterial populations affiliated with the Roseobacter group and additional deep‐branching alphaproteobacterial lineages. In contrast, isolates from the ANG and ECs contained only a few alpha‐proteobacteria of the Roseobacter group compared with several gamma‐proteobacterial isolates, mostly Shewanella and Pseudoalteromonas species. Most of the ANG‐associated bacterial populations were also found within the ECs of L. pealei . The molecular approach allowed the visualization of alpha‐proteobacteria as major constituents of a bacterial symbiosis within the reproductive system of the Loliginidae. |
format |
Article in Journal/Newspaper |
author |
Barbieri, Elena Paster, Bruce J. Hughes, Deborah Zurek, Ludek Moser, Duane P. Teske, Andreas Sogin, Mitchell L. |
spellingShingle |
Barbieri, Elena Paster, Bruce J. Hughes, Deborah Zurek, Ludek Moser, Duane P. Teske, Andreas Sogin, Mitchell L. Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda: Loliginidae) |
author_facet |
Barbieri, Elena Paster, Bruce J. Hughes, Deborah Zurek, Ludek Moser, Duane P. Teske, Andreas Sogin, Mitchell L. |
author_sort |
Barbieri, Elena |
title |
Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda: Loliginidae) |
title_short |
Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda: Loliginidae) |
title_full |
Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda: Loliginidae) |
title_fullStr |
Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda: Loliginidae) |
title_full_unstemmed |
Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda: Loliginidae) |
title_sort |
phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid loligo pealei (cephalopoda: loliginidae) |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1046/j.1462-2920.2001.00172.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1462-2920.2001.00172.x http://onlinelibrary.wiley.com/wol1/doi/10.1046/j.1462-2920.2001.00172.x/fullpdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Environmental Microbiology volume 3, issue 3, page 151-167 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1462-2920.2001.00172.x |
container_title |
Environmental Microbiology |
container_volume |
3 |
container_issue |
3 |
container_start_page |
151 |
op_container_end_page |
167 |
_version_ |
1810463619312254976 |