Genotypic Analysis of Russian Isolates of Phytophthora infestans from the Moscow Region, Siberia and Far East
Phytophthora infestans samples were collected during 1997 and 1998 at multiple sites in Russia from Sakhalin Island in the Far East across Siberia (nine sites, 160 isolates) to the Moscow region (four sites, 325 isolates). In addition, 12 isolates that were obtained previously were included. All iso...
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crwiley:10.1046/j.1439-0434.2001.00642.x 2024-06-02T08:13:56+00:00 Genotypic Analysis of Russian Isolates of Phytophthora infestans from the Moscow Region, Siberia and Far East Elansky, S. Smirnov, A. Dyakov, Y. Dolgova, A. Filippov, A. Kozlovsky, B. Kozlovskaya, I. Russo, P. Smart, C. Fry, W. 2001 http://dx.doi.org/10.1046/j.1439-0434.2001.00642.x http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1439-0434.2001.00642.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1439-0434.2001.00642.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phytopathology volume 149, issue 10, page 605-611 ISSN 0931-1785 1439-0434 journal-article 2001 crwiley https://doi.org/10.1046/j.1439-0434.2001.00642.x 2024-05-03T11:10:18Z Phytophthora infestans samples were collected during 1997 and 1998 at multiple sites in Russia from Sakhalin Island in the Far East across Siberia (nine sites, 160 isolates) to the Moscow region (four sites, 325 isolates). In addition, 12 isolates that were obtained previously were included. All isolates were analysed for mating type, and sensitivity to metalaxyl. Isolates from within any of the nine sites outside of the Moscow region were monomorphic for mating type and nearly monomorphic for metalaxyl resistance. In contrast, both A1 and A2 isolates were detected in the Moscow region, and these isolates were also polymorphic for metalaxyl resistance. In two sites in Siberia only A2 mating type strains were detected, in the other six sites in Siberia and in Sakahlin Island, only A1 mating types were detected. A subset of isolates ( n =191) was also analysed for pathotype (virulence to 10 R‐genes, each in a distinct differential genotype). All isolates were highly complex (mean number of virulences approximately 8.4 of a maximum number of 10). All isolates ( n =43) from Sakahlin Island were virulent to all 10 of the R‐genes tested. A further subset of isolates ( n =70, including 12 isolates collected before 1997) was analysed for genotype at the Glucose‐ 6‐phosphate isomerase and Peptidase loci, mtDNA haplotypes, and RFLP pattern using the RG57 probe. The US‐1 clonal lineage (previously dominant) was not detected in the 1997–98 sample. The populations of P. infestans near Moscow in 1997 and 1998 was highly diverse with 15 unique genotypes (including both mating types) among a sample of 18 isolates. In contrast, the populations of P. infestans in Siberia had limited diversity, with only three multilocus genotypes detected and most populations were dominated by the SIB‐1 clonal lineage. This lineage accounted for 31 of the 39 strains collected in Siberia that were assayed for multilocus genotype. Article in Journal/Newspaper Sakhalin Siberia Wiley Online Library Journal of Phytopathology 149 10 605 611 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Phytophthora infestans samples were collected during 1997 and 1998 at multiple sites in Russia from Sakhalin Island in the Far East across Siberia (nine sites, 160 isolates) to the Moscow region (four sites, 325 isolates). In addition, 12 isolates that were obtained previously were included. All isolates were analysed for mating type, and sensitivity to metalaxyl. Isolates from within any of the nine sites outside of the Moscow region were monomorphic for mating type and nearly monomorphic for metalaxyl resistance. In contrast, both A1 and A2 isolates were detected in the Moscow region, and these isolates were also polymorphic for metalaxyl resistance. In two sites in Siberia only A2 mating type strains were detected, in the other six sites in Siberia and in Sakahlin Island, only A1 mating types were detected. A subset of isolates ( n =191) was also analysed for pathotype (virulence to 10 R‐genes, each in a distinct differential genotype). All isolates were highly complex (mean number of virulences approximately 8.4 of a maximum number of 10). All isolates ( n =43) from Sakahlin Island were virulent to all 10 of the R‐genes tested. A further subset of isolates ( n =70, including 12 isolates collected before 1997) was analysed for genotype at the Glucose‐ 6‐phosphate isomerase and Peptidase loci, mtDNA haplotypes, and RFLP pattern using the RG57 probe. The US‐1 clonal lineage (previously dominant) was not detected in the 1997–98 sample. The populations of P. infestans near Moscow in 1997 and 1998 was highly diverse with 15 unique genotypes (including both mating types) among a sample of 18 isolates. In contrast, the populations of P. infestans in Siberia had limited diversity, with only three multilocus genotypes detected and most populations were dominated by the SIB‐1 clonal lineage. This lineage accounted for 31 of the 39 strains collected in Siberia that were assayed for multilocus genotype. |
format |
Article in Journal/Newspaper |
author |
Elansky, S. Smirnov, A. Dyakov, Y. Dolgova, A. Filippov, A. Kozlovsky, B. Kozlovskaya, I. Russo, P. Smart, C. Fry, W. |
spellingShingle |
Elansky, S. Smirnov, A. Dyakov, Y. Dolgova, A. Filippov, A. Kozlovsky, B. Kozlovskaya, I. Russo, P. Smart, C. Fry, W. Genotypic Analysis of Russian Isolates of Phytophthora infestans from the Moscow Region, Siberia and Far East |
author_facet |
Elansky, S. Smirnov, A. Dyakov, Y. Dolgova, A. Filippov, A. Kozlovsky, B. Kozlovskaya, I. Russo, P. Smart, C. Fry, W. |
author_sort |
Elansky, S. |
title |
Genotypic Analysis of Russian Isolates of Phytophthora infestans from the Moscow Region, Siberia and Far East |
title_short |
Genotypic Analysis of Russian Isolates of Phytophthora infestans from the Moscow Region, Siberia and Far East |
title_full |
Genotypic Analysis of Russian Isolates of Phytophthora infestans from the Moscow Region, Siberia and Far East |
title_fullStr |
Genotypic Analysis of Russian Isolates of Phytophthora infestans from the Moscow Region, Siberia and Far East |
title_full_unstemmed |
Genotypic Analysis of Russian Isolates of Phytophthora infestans from the Moscow Region, Siberia and Far East |
title_sort |
genotypic analysis of russian isolates of phytophthora infestans from the moscow region, siberia and far east |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1046/j.1439-0434.2001.00642.x http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1439-0434.2001.00642.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1439-0434.2001.00642.x |
genre |
Sakhalin Siberia |
genre_facet |
Sakhalin Siberia |
op_source |
Journal of Phytopathology volume 149, issue 10, page 605-611 ISSN 0931-1785 1439-0434 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1439-0434.2001.00642.x |
container_title |
Journal of Phytopathology |
container_volume |
149 |
container_issue |
10 |
container_start_page |
605 |
op_container_end_page |
611 |
_version_ |
1800737597839900672 |