A DNA ligase from the psychrophile Pseudoalteromonas haloplanktisgives insights into the adaptation of proteins to low temperatures
The cloning, overexpression and characterization of a cold‐adapted DNA ligase from the Antarctic sea water bacterium Pseudoalteromonas haloplanktis are described. Protein sequence analysis revealed that the cold‐adapted Ph DNA ligase shows a significant level of sequence similarity to other NAD + ‐d...
Published in: | European Journal of Biochemistry |
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Online Access: | http://dx.doi.org/10.1046/j.1432-1327.2000.01377.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.2000.01377.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.2000.01377.x |
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crwiley:10.1046/j.1432-1327.2000.01377.x 2024-06-23T07:47:30+00:00 A DNA ligase from the psychrophile Pseudoalteromonas haloplanktisgives insights into the adaptation of proteins to low temperatures Georlette, D. Jónsson, Z. O. Van Petegem, F. Chessa, J.‐P. Van Beeumen, J. Hübscher, U. Gerday, C. 2000 http://dx.doi.org/10.1046/j.1432-1327.2000.01377.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.2000.01377.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.2000.01377.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor European Journal of Biochemistry volume 267, issue 12, page 3502-3512 ISSN 0014-2956 1432-1033 journal-article 2000 crwiley https://doi.org/10.1046/j.1432-1327.2000.01377.x 2024-06-11T04:51:04Z The cloning, overexpression and characterization of a cold‐adapted DNA ligase from the Antarctic sea water bacterium Pseudoalteromonas haloplanktis are described. Protein sequence analysis revealed that the cold‐adapted Ph DNA ligase shows a significant level of sequence similarity to other NAD + ‐dependent DNA ligases and contains several previously described sequence motifs. Also, a decreased level of arginine and proline residues in Ph DNA ligase could be involved in the cold‐adaptation strategy. Moreover, 3D modelling of the N‐terminal domain of Ph DNA ligase clearly indicates that this domain is destabilized compared with its thermophilic homologue. The recombinant Ph DNA ligase was overexpressed in Escherichia coli and purified to homogeneity. Mass spectroscopy experiments indicated that the purified enzyme is mainly in an adenylated form with a molecular mass of 74 593 Da. Ph DNA ligase shows similar overall catalytic properties to other NAD + ‐dependent DNA ligases but is a cold‐adapted enzyme as its catalytic efficiency ( k cat / K m ) at low and moderate temperatures is higher than that of its mesophilic counterpart E. coli DNA ligase. A kinetic comparison of three enzymes adapted to different temperatures ( P. haloplanktis , E. coli and Thermus scotoductus DNA ligases) indicated that an increased k cat is the most important adaptive parameter for enzymatic activity at low temperatures, whereas a decreased K m for the nicked DNA substrate seems to allow T. scotoductus DNA ligase to work efficiently at high temperatures. Besides being useful for investigation of the adaptation of enzymes to extreme temperatures, P. haloplanktis DNA ligase, which is very efficient at low temperatures, offers a novel tool for biotechnology. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The Antarctic European Journal of Biochemistry 267 12 3502 3512 |
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Open Polar |
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Wiley Online Library |
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language |
English |
description |
The cloning, overexpression and characterization of a cold‐adapted DNA ligase from the Antarctic sea water bacterium Pseudoalteromonas haloplanktis are described. Protein sequence analysis revealed that the cold‐adapted Ph DNA ligase shows a significant level of sequence similarity to other NAD + ‐dependent DNA ligases and contains several previously described sequence motifs. Also, a decreased level of arginine and proline residues in Ph DNA ligase could be involved in the cold‐adaptation strategy. Moreover, 3D modelling of the N‐terminal domain of Ph DNA ligase clearly indicates that this domain is destabilized compared with its thermophilic homologue. The recombinant Ph DNA ligase was overexpressed in Escherichia coli and purified to homogeneity. Mass spectroscopy experiments indicated that the purified enzyme is mainly in an adenylated form with a molecular mass of 74 593 Da. Ph DNA ligase shows similar overall catalytic properties to other NAD + ‐dependent DNA ligases but is a cold‐adapted enzyme as its catalytic efficiency ( k cat / K m ) at low and moderate temperatures is higher than that of its mesophilic counterpart E. coli DNA ligase. A kinetic comparison of three enzymes adapted to different temperatures ( P. haloplanktis , E. coli and Thermus scotoductus DNA ligases) indicated that an increased k cat is the most important adaptive parameter for enzymatic activity at low temperatures, whereas a decreased K m for the nicked DNA substrate seems to allow T. scotoductus DNA ligase to work efficiently at high temperatures. Besides being useful for investigation of the adaptation of enzymes to extreme temperatures, P. haloplanktis DNA ligase, which is very efficient at low temperatures, offers a novel tool for biotechnology. |
format |
Article in Journal/Newspaper |
author |
Georlette, D. Jónsson, Z. O. Van Petegem, F. Chessa, J.‐P. Van Beeumen, J. Hübscher, U. Gerday, C. |
spellingShingle |
Georlette, D. Jónsson, Z. O. Van Petegem, F. Chessa, J.‐P. Van Beeumen, J. Hübscher, U. Gerday, C. A DNA ligase from the psychrophile Pseudoalteromonas haloplanktisgives insights into the adaptation of proteins to low temperatures |
author_facet |
Georlette, D. Jónsson, Z. O. Van Petegem, F. Chessa, J.‐P. Van Beeumen, J. Hübscher, U. Gerday, C. |
author_sort |
Georlette, D. |
title |
A DNA ligase from the psychrophile Pseudoalteromonas haloplanktisgives insights into the adaptation of proteins to low temperatures |
title_short |
A DNA ligase from the psychrophile Pseudoalteromonas haloplanktisgives insights into the adaptation of proteins to low temperatures |
title_full |
A DNA ligase from the psychrophile Pseudoalteromonas haloplanktisgives insights into the adaptation of proteins to low temperatures |
title_fullStr |
A DNA ligase from the psychrophile Pseudoalteromonas haloplanktisgives insights into the adaptation of proteins to low temperatures |
title_full_unstemmed |
A DNA ligase from the psychrophile Pseudoalteromonas haloplanktisgives insights into the adaptation of proteins to low temperatures |
title_sort |
dna ligase from the psychrophile pseudoalteromonas haloplanktisgives insights into the adaptation of proteins to low temperatures |
publisher |
Wiley |
publishDate |
2000 |
url |
http://dx.doi.org/10.1046/j.1432-1327.2000.01377.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.2000.01377.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.2000.01377.x |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
European Journal of Biochemistry volume 267, issue 12, page 3502-3512 ISSN 0014-2956 1432-1033 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1432-1327.2000.01377.x |
container_title |
European Journal of Biochemistry |
container_volume |
267 |
container_issue |
12 |
container_start_page |
3502 |
op_container_end_page |
3512 |
_version_ |
1802651618055815168 |