Aspartate aminotransferase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC 125

• Published in: European Journal of Biochemistry
• Main Authors: Birolo, Leila, Tutino, M. Luisa, Fontanella, Bianca, Gerday, Charles, Mainolfi, Katia, Pascarella, Stefano, Sannia, Giovanni, Vinci, Floriana, Marino, Gennaro
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2000
• Subjects: Antarctic; Tac; The Antarctic; Antarc*
• Online Access: http://dx.doi.org/10.1046/j.1432-1327.2000.01299.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.2000.01299.x; https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.2000.01299.x

The gene encoding aspartate aminotransferase from the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC 125 was cloned, sequenced and overexpressed in Escherichia coli . The recombinant protein ( Ph AspAT) was characterized both at the structural and functional level in comparison with the...