Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod
Kininogens are multifunctional proteins found so far mainly in mammals. They carry vasoactive kinins as well as participate in defense, blood coagulation and the acute phase response. In this study, novel kininogens were isolated from Atlantic cod ( Gadus morhua L.) and spotted wolffish( Anarhichas...
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crwiley:10.1046/j.1432-1033.2002.02927.x 2024-06-02T08:03:08+00:00 Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod Ylönen, Anne Helin, Jari Bøgwald, Jarl Jaakola, Anu Rinne, Ari Kalkkinen, Nisse 2002 http://dx.doi.org/10.1046/j.1432-1033.2002.02927.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1033.2002.02927.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1033.2002.02927.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor European Journal of Biochemistry volume 269, issue 11, page 2639-2646 ISSN 0014-2956 1432-1033 journal-article 2002 crwiley https://doi.org/10.1046/j.1432-1033.2002.02927.x 2024-05-03T11:05:02Z Kininogens are multifunctional proteins found so far mainly in mammals. They carry vasoactive kinins as well as participate in defense, blood coagulation and the acute phase response. In this study, novel kininogens were isolated from Atlantic cod ( Gadus morhua L.) and spotted wolffish( Anarhichas minor ) by papain‐affinity chromatography. The molecular mass of cod kininogen determined by MALDI‐TOF mass spectrometry to be 51.0 kDa and it had pI values of 3.6, 3.9 and 4.4. The molecular mass of wolffish kininogen was 45.8 kDa and it had pI values of 4.1, 4.3, 4.35 and 4.4. Partial amino‐acid sequences determined from both kininogens showed clear homology with previously determined kininogen sequences. Both kininogens were found to inhibit cysteine proteinases like papain and ficin but they had no effect on trypsin, a serine proteinase. Wolffish kininogen carried α2,3‐sialylated biantennary and triantennary N‐glycans with extensive sialic acid O‐acetylation. Cod kininogen carried similar glycan structures but about 1/3 of its glycans carried sulfate at their N ‐acetylglucosamine units. Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library European Journal of Biochemistry 269 11 2639 2646 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Kininogens are multifunctional proteins found so far mainly in mammals. They carry vasoactive kinins as well as participate in defense, blood coagulation and the acute phase response. In this study, novel kininogens were isolated from Atlantic cod ( Gadus morhua L.) and spotted wolffish( Anarhichas minor ) by papain‐affinity chromatography. The molecular mass of cod kininogen determined by MALDI‐TOF mass spectrometry to be 51.0 kDa and it had pI values of 3.6, 3.9 and 4.4. The molecular mass of wolffish kininogen was 45.8 kDa and it had pI values of 4.1, 4.3, 4.35 and 4.4. Partial amino‐acid sequences determined from both kininogens showed clear homology with previously determined kininogen sequences. Both kininogens were found to inhibit cysteine proteinases like papain and ficin but they had no effect on trypsin, a serine proteinase. Wolffish kininogen carried α2,3‐sialylated biantennary and triantennary N‐glycans with extensive sialic acid O‐acetylation. Cod kininogen carried similar glycan structures but about 1/3 of its glycans carried sulfate at their N ‐acetylglucosamine units. |
format |
Article in Journal/Newspaper |
author |
Ylönen, Anne Helin, Jari Bøgwald, Jarl Jaakola, Anu Rinne, Ari Kalkkinen, Nisse |
spellingShingle |
Ylönen, Anne Helin, Jari Bøgwald, Jarl Jaakola, Anu Rinne, Ari Kalkkinen, Nisse Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod |
author_facet |
Ylönen, Anne Helin, Jari Bøgwald, Jarl Jaakola, Anu Rinne, Ari Kalkkinen, Nisse |
author_sort |
Ylönen, Anne |
title |
Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod |
title_short |
Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod |
title_full |
Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod |
title_fullStr |
Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod |
title_full_unstemmed |
Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod |
title_sort |
purification and characterization of novel kininogens from spotted wolffish and atlantic cod |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1046/j.1432-1033.2002.02927.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1033.2002.02927.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1033.2002.02927.x |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
European Journal of Biochemistry volume 269, issue 11, page 2639-2646 ISSN 0014-2956 1432-1033 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1432-1033.2002.02927.x |
container_title |
European Journal of Biochemistry |
container_volume |
269 |
container_issue |
11 |
container_start_page |
2639 |
op_container_end_page |
2646 |
_version_ |
1800747610920714240 |