Expression and Analysis of Recombinant Salmon Parvalbumin, the Major Allergen in Atlantic Salmon ( Salmo salar)
The parvalbumin from white muscle of Atlantic salmon was previously found to be a major allergen, and designated Sal s1 . Two distinct cDNAs, 14.1 and 24.1, which comprise the entire parvalbumin‐encoding regions, were cloned, revealing transcripts from two different parvalbumin genes. In the present...
Published in: | Scandinavian Journal of Immunology |
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Online Access: | http://dx.doi.org/10.1046/j.1365-3083.1999.00637.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-3083.1999.00637.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-3083.1999.00637.x |
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crwiley:10.1046/j.1365-3083.1999.00637.x 2024-06-02T08:03:33+00:00 Expression and Analysis of Recombinant Salmon Parvalbumin, the Major Allergen in Atlantic Salmon ( Salmo salar) Van Do Hordvik Endresen Elsayed 1999 http://dx.doi.org/10.1046/j.1365-3083.1999.00637.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-3083.1999.00637.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-3083.1999.00637.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Scandinavian Journal of Immunology volume 50, issue 6, page 619-625 ISSN 0300-9475 1365-3083 journal-article 1999 crwiley https://doi.org/10.1046/j.1365-3083.1999.00637.x 2024-05-03T11:20:16Z The parvalbumin from white muscle of Atlantic salmon was previously found to be a major allergen, and designated Sal s1 . Two distinct cDNAs, 14.1 and 24.1, which comprise the entire parvalbumin‐encoding regions, were cloned, revealing transcripts from two different parvalbumin genes. In the present study, the protein‐coding regions of these cDNAs were subcloned into an Escherichia coli expression vector (pET‐19b). Both proteins were expressed and the generated target proteins were localized in both soluble and insoluble fractions of the expression host. The recombinant products in the soluble fraction were purified using the His tag‐purification system and analysed on Western blots with anti‐salmon parvalbumin polyclonal rabbit sera and sera from patients allergic to fish. Both recombinant products (His 10 ‐14.1 and His 10 ‐24.1) reacted positively with salmon parvalbumin‐specific immunogloblin G (IgG) from rabbits, and with specific immunoglobulin E (IgE) from the sera of six fish‐allergic patients. The allergenicity of His 10 ‐14.1 was confirmed using enzyme‐linked immunosorbent assay (ELISA). The 14.1 cDNA of salmon parvalbumin was shown to be the dominant type represented in a muscle cDNA library. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Scandinavian Journal of Immunology 50 6 619 625 |
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Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
The parvalbumin from white muscle of Atlantic salmon was previously found to be a major allergen, and designated Sal s1 . Two distinct cDNAs, 14.1 and 24.1, which comprise the entire parvalbumin‐encoding regions, were cloned, revealing transcripts from two different parvalbumin genes. In the present study, the protein‐coding regions of these cDNAs were subcloned into an Escherichia coli expression vector (pET‐19b). Both proteins were expressed and the generated target proteins were localized in both soluble and insoluble fractions of the expression host. The recombinant products in the soluble fraction were purified using the His tag‐purification system and analysed on Western blots with anti‐salmon parvalbumin polyclonal rabbit sera and sera from patients allergic to fish. Both recombinant products (His 10 ‐14.1 and His 10 ‐24.1) reacted positively with salmon parvalbumin‐specific immunogloblin G (IgG) from rabbits, and with specific immunoglobulin E (IgE) from the sera of six fish‐allergic patients. The allergenicity of His 10 ‐14.1 was confirmed using enzyme‐linked immunosorbent assay (ELISA). The 14.1 cDNA of salmon parvalbumin was shown to be the dominant type represented in a muscle cDNA library. |
format |
Article in Journal/Newspaper |
author |
Van Do Hordvik Endresen Elsayed |
spellingShingle |
Van Do Hordvik Endresen Elsayed Expression and Analysis of Recombinant Salmon Parvalbumin, the Major Allergen in Atlantic Salmon ( Salmo salar) |
author_facet |
Van Do Hordvik Endresen Elsayed |
author_sort |
Van Do |
title |
Expression and Analysis of Recombinant Salmon Parvalbumin, the Major Allergen in Atlantic Salmon ( Salmo salar) |
title_short |
Expression and Analysis of Recombinant Salmon Parvalbumin, the Major Allergen in Atlantic Salmon ( Salmo salar) |
title_full |
Expression and Analysis of Recombinant Salmon Parvalbumin, the Major Allergen in Atlantic Salmon ( Salmo salar) |
title_fullStr |
Expression and Analysis of Recombinant Salmon Parvalbumin, the Major Allergen in Atlantic Salmon ( Salmo salar) |
title_full_unstemmed |
Expression and Analysis of Recombinant Salmon Parvalbumin, the Major Allergen in Atlantic Salmon ( Salmo salar) |
title_sort |
expression and analysis of recombinant salmon parvalbumin, the major allergen in atlantic salmon ( salmo salar) |
publisher |
Wiley |
publishDate |
1999 |
url |
http://dx.doi.org/10.1046/j.1365-3083.1999.00637.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-3083.1999.00637.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-3083.1999.00637.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Scandinavian Journal of Immunology volume 50, issue 6, page 619-625 ISSN 0300-9475 1365-3083 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-3083.1999.00637.x |
container_title |
Scandinavian Journal of Immunology |
container_volume |
50 |
container_issue |
6 |
container_start_page |
619 |
op_container_end_page |
625 |
_version_ |
1800748102744801280 |