Kinetics of amino acid uptake by ectomycorrhizal roots
It is well established that ectomycorrhizal fungi can use amino acids as nitrogen and carbon sources, but data on the kinetic properties of amino acid uptake systems of ectomycorrhizal systems are scarce. Using 14 C‐labelled compounds we have determined the kinetics of uptake of amino acids by excis...
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crwiley:10.1046/j.1365-3040.1999.00385.x 2024-06-02T08:12:11+00:00 Kinetics of amino acid uptake by ectomycorrhizal roots WALLENDA, T. READ, D. J. 1999 http://dx.doi.org/10.1046/j.1365-3040.1999.00385.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-3040.1999.00385.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-3040.1999.00385.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Plant, Cell & Environment volume 22, issue 2, page 179-187 ISSN 0140-7791 1365-3040 journal-article 1999 crwiley https://doi.org/10.1046/j.1365-3040.1999.00385.x 2024-05-03T11:39:45Z It is well established that ectomycorrhizal fungi can use amino acids as nitrogen and carbon sources, but data on the kinetic properties of amino acid uptake systems of ectomycorrhizal systems are scarce. Using 14 C‐labelled compounds we have determined the kinetics of uptake of amino acids by excised ectomycorrhizal roots for a range of distinct mycorrhizal types from three tree species, beech, spruce, and pine. All mycorrhizal types examined took up amino acids via high‐affinity transport systems ( K M values ranging from 19 to 233 mmol m –3 ). A comparative analysis of kinetic parameters for uptake of amino acids and the ammonium analogue methylammonium showed that ectomycorrhizal roots have similar or even higher affinities (lower K M values) for the amino acids, indicating that absorption of these organic forms of nitrogen (N) can contribute significantly to total N uptake by ectomycorrhizal plants. Analysis of amino acid uptake by ectomycorrhizal roots collected along a European north/south gradient of increasing mineral N pollution from northern Sweden to south Germany revealed no obvious trend in the uptake capabilities for amino acids by ectomycorrhizal roots in relation to the location of the sampling site on this gradient. Rather, the fungal species forming a particular morphotype was the factor determining uptake kinetics. It can therefore be deduced that the species composition of the fungal community will contribute significantly to the functional diversity of a population of mycorrhizal roots. Article in Journal/Newspaper Northern Sweden Wiley Online Library Plant, Cell & Environment 22 2 179 187 |
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English |
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It is well established that ectomycorrhizal fungi can use amino acids as nitrogen and carbon sources, but data on the kinetic properties of amino acid uptake systems of ectomycorrhizal systems are scarce. Using 14 C‐labelled compounds we have determined the kinetics of uptake of amino acids by excised ectomycorrhizal roots for a range of distinct mycorrhizal types from three tree species, beech, spruce, and pine. All mycorrhizal types examined took up amino acids via high‐affinity transport systems ( K M values ranging from 19 to 233 mmol m –3 ). A comparative analysis of kinetic parameters for uptake of amino acids and the ammonium analogue methylammonium showed that ectomycorrhizal roots have similar or even higher affinities (lower K M values) for the amino acids, indicating that absorption of these organic forms of nitrogen (N) can contribute significantly to total N uptake by ectomycorrhizal plants. Analysis of amino acid uptake by ectomycorrhizal roots collected along a European north/south gradient of increasing mineral N pollution from northern Sweden to south Germany revealed no obvious trend in the uptake capabilities for amino acids by ectomycorrhizal roots in relation to the location of the sampling site on this gradient. Rather, the fungal species forming a particular morphotype was the factor determining uptake kinetics. It can therefore be deduced that the species composition of the fungal community will contribute significantly to the functional diversity of a population of mycorrhizal roots. |
format |
Article in Journal/Newspaper |
author |
WALLENDA, T. READ, D. J. |
spellingShingle |
WALLENDA, T. READ, D. J. Kinetics of amino acid uptake by ectomycorrhizal roots |
author_facet |
WALLENDA, T. READ, D. J. |
author_sort |
WALLENDA, T. |
title |
Kinetics of amino acid uptake by ectomycorrhizal roots |
title_short |
Kinetics of amino acid uptake by ectomycorrhizal roots |
title_full |
Kinetics of amino acid uptake by ectomycorrhizal roots |
title_fullStr |
Kinetics of amino acid uptake by ectomycorrhizal roots |
title_full_unstemmed |
Kinetics of amino acid uptake by ectomycorrhizal roots |
title_sort |
kinetics of amino acid uptake by ectomycorrhizal roots |
publisher |
Wiley |
publishDate |
1999 |
url |
http://dx.doi.org/10.1046/j.1365-3040.1999.00385.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-3040.1999.00385.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-3040.1999.00385.x |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Plant, Cell & Environment volume 22, issue 2, page 179-187 ISSN 0140-7791 1365-3040 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-3040.1999.00385.x |
container_title |
Plant, Cell & Environment |
container_volume |
22 |
container_issue |
2 |
container_start_page |
179 |
op_container_end_page |
187 |
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1800758550462464000 |