The influence of diet on faecal DNA amplification and sex identification in brown bears ( Ursus arctos)
Abstract To evaluate the influence of diet on faecal DNA amplification, 11 captive brown bears ( Ursus arctos ) were placed on six restricted diets: grass ( Trifolium spp., Haplopappus hirtus and Poa pratensis ), alfalfa ( Lupinus spp.), carrots ( Daucus spp.), white‐tailed deer ( Odocoileus virgini...
Published in: | Molecular Ecology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2003
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1046/j.1365-294x.2003.01863.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294X.2003.01863.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294X.2003.01863.x https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-294X.2003.01863.x |
id |
crwiley:10.1046/j.1365-294x.2003.01863.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1046/j.1365-294x.2003.01863.x 2024-09-09T20:12:46+00:00 The influence of diet on faecal DNA amplification and sex identification in brown bears ( Ursus arctos) Murphy, Melanie A. Waits, Lisette P. Kendall, Katherine C. 2003 http://dx.doi.org/10.1046/j.1365-294x.2003.01863.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294X.2003.01863.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294X.2003.01863.x https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-294X.2003.01863.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology volume 12, issue 8, page 2261-2265 ISSN 0962-1083 1365-294X journal-article 2003 crwiley https://doi.org/10.1046/j.1365-294x.2003.01863.x 2024-07-30T04:24:16Z Abstract To evaluate the influence of diet on faecal DNA amplification, 11 captive brown bears ( Ursus arctos ) were placed on six restricted diets: grass ( Trifolium spp., Haplopappus hirtus and Poa pratensis ), alfalfa ( Lupinus spp.), carrots ( Daucus spp.), white‐tailed deer ( Odocoileus virginianus ), blueberries ( Vaccinium spp.) and salmon ( Salmo spp.). DNA was extracted from 50 faecal samples of each restricted diet, and amplification of brown bear DNA was attempted for a mitochondrial DNA (mtDNA) locus and nuclear DNA (nDNA) locus. For mtDNA, no significant differences were observed in amplification success rates across diets. For nDNA, amplification success rates for salmon diet extracts were significantly lower than all other diet extracts ( P < 0.001). To evaluate the accuracy of faecal DNA sex identification when female carnivores consume male mammalian prey, female bears were fed male white‐tailed deer. Four of 10 extracts amplified, and all extracts were incorrectly scored as male due to amplification of X and Y‐chromosome fragments. The potential biases highlighted in this study have broad implications for researchers using faecal DNA for individual and sex identification, and should be evaluated in other species. Article in Journal/Newspaper Ursus arctos Wiley Online Library Molecular Ecology 12 8 2261 2265 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract To evaluate the influence of diet on faecal DNA amplification, 11 captive brown bears ( Ursus arctos ) were placed on six restricted diets: grass ( Trifolium spp., Haplopappus hirtus and Poa pratensis ), alfalfa ( Lupinus spp.), carrots ( Daucus spp.), white‐tailed deer ( Odocoileus virginianus ), blueberries ( Vaccinium spp.) and salmon ( Salmo spp.). DNA was extracted from 50 faecal samples of each restricted diet, and amplification of brown bear DNA was attempted for a mitochondrial DNA (mtDNA) locus and nuclear DNA (nDNA) locus. For mtDNA, no significant differences were observed in amplification success rates across diets. For nDNA, amplification success rates for salmon diet extracts were significantly lower than all other diet extracts ( P < 0.001). To evaluate the accuracy of faecal DNA sex identification when female carnivores consume male mammalian prey, female bears were fed male white‐tailed deer. Four of 10 extracts amplified, and all extracts were incorrectly scored as male due to amplification of X and Y‐chromosome fragments. The potential biases highlighted in this study have broad implications for researchers using faecal DNA for individual and sex identification, and should be evaluated in other species. |
format |
Article in Journal/Newspaper |
author |
Murphy, Melanie A. Waits, Lisette P. Kendall, Katherine C. |
spellingShingle |
Murphy, Melanie A. Waits, Lisette P. Kendall, Katherine C. The influence of diet on faecal DNA amplification and sex identification in brown bears ( Ursus arctos) |
author_facet |
Murphy, Melanie A. Waits, Lisette P. Kendall, Katherine C. |
author_sort |
Murphy, Melanie A. |
title |
The influence of diet on faecal DNA amplification and sex identification in brown bears ( Ursus arctos) |
title_short |
The influence of diet on faecal DNA amplification and sex identification in brown bears ( Ursus arctos) |
title_full |
The influence of diet on faecal DNA amplification and sex identification in brown bears ( Ursus arctos) |
title_fullStr |
The influence of diet on faecal DNA amplification and sex identification in brown bears ( Ursus arctos) |
title_full_unstemmed |
The influence of diet on faecal DNA amplification and sex identification in brown bears ( Ursus arctos) |
title_sort |
influence of diet on faecal dna amplification and sex identification in brown bears ( ursus arctos) |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1046/j.1365-294x.2003.01863.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294X.2003.01863.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294X.2003.01863.x https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-294X.2003.01863.x |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Molecular Ecology volume 12, issue 8, page 2261-2265 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-294x.2003.01863.x |
container_title |
Molecular Ecology |
container_volume |
12 |
container_issue |
8 |
container_start_page |
2261 |
op_container_end_page |
2265 |
_version_ |
1809947384740839424 |