A DNA‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators
Abstract Accurate identification of species that are consumed by vertebrate predators is necessary for understanding marine food webs. Morphological methods for identifying prey components after consumption often fail to make accurate identifications of invertebrates because prey morphology becomes...
Published in: | Molecular Ecology |
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Online Access: | http://dx.doi.org/10.1046/j.1365-294x.2002.01641.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294X.2002.01641.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294X.2002.01641.x |
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crwiley:10.1046/j.1365-294x.2002.01641.x 2024-06-02T07:54:20+00:00 A DNA‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators Jarman, S. N. Gales, N. J. Tierney, M. Gill, P. C. Elliott, N. G. 2002 http://dx.doi.org/10.1046/j.1365-294x.2002.01641.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294X.2002.01641.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294X.2002.01641.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology volume 11, issue 12, page 2679-2690 ISSN 0962-1083 1365-294X journal-article 2002 crwiley https://doi.org/10.1046/j.1365-294x.2002.01641.x 2024-05-03T11:43:18Z Abstract Accurate identification of species that are consumed by vertebrate predators is necessary for understanding marine food webs. Morphological methods for identifying prey components after consumption often fail to make accurate identifications of invertebrates because prey morphology becomes damaged during capture, ingestion and digestion. Another disadvantage of morphological methods for prey identification is that they often involve sampling procedures that are disruptive for the predator, such as stomach flushing or lethal collection. We have developed a DNA‐based method for identifying species of krill (Crustacea: Malacostraca), an enormously abundant group of invertebrates that are directly consumed by many groups of marine vertebrates. The DNA‐based approach allows identification of krill species present in samples of vertebrate stomach contents, vomit, and, more importantly, faeces. Utilizing samples of faeces from vertebrate predators minimizes the impact of dietary studies on the subject animals. We demonstrate our method first on samples of Adelie penguin ( Pygoscelis adeliae ) stomach contents, where DNA‐based species identification can be confirmed by prey morphology. We then apply the method to faeces of Adelie penguins and to faeces of the endangered pygmy blue whale ( Balaenoptera musculus brevicauda ). In each of these cases, krill species consumed by the predators could be identified from their DNA present in faeces or stomach contents. Article in Journal/Newspaper Adelie penguin Balaenoptera musculus Blue whale Pygoscelis adeliae Wiley Online Library Molecular Ecology 11 12 2679 2690 |
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Wiley Online Library |
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English |
description |
Abstract Accurate identification of species that are consumed by vertebrate predators is necessary for understanding marine food webs. Morphological methods for identifying prey components after consumption often fail to make accurate identifications of invertebrates because prey morphology becomes damaged during capture, ingestion and digestion. Another disadvantage of morphological methods for prey identification is that they often involve sampling procedures that are disruptive for the predator, such as stomach flushing or lethal collection. We have developed a DNA‐based method for identifying species of krill (Crustacea: Malacostraca), an enormously abundant group of invertebrates that are directly consumed by many groups of marine vertebrates. The DNA‐based approach allows identification of krill species present in samples of vertebrate stomach contents, vomit, and, more importantly, faeces. Utilizing samples of faeces from vertebrate predators minimizes the impact of dietary studies on the subject animals. We demonstrate our method first on samples of Adelie penguin ( Pygoscelis adeliae ) stomach contents, where DNA‐based species identification can be confirmed by prey morphology. We then apply the method to faeces of Adelie penguins and to faeces of the endangered pygmy blue whale ( Balaenoptera musculus brevicauda ). In each of these cases, krill species consumed by the predators could be identified from their DNA present in faeces or stomach contents. |
format |
Article in Journal/Newspaper |
author |
Jarman, S. N. Gales, N. J. Tierney, M. Gill, P. C. Elliott, N. G. |
spellingShingle |
Jarman, S. N. Gales, N. J. Tierney, M. Gill, P. C. Elliott, N. G. A DNA‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators |
author_facet |
Jarman, S. N. Gales, N. J. Tierney, M. Gill, P. C. Elliott, N. G. |
author_sort |
Jarman, S. N. |
title |
A DNA‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators |
title_short |
A DNA‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators |
title_full |
A DNA‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators |
title_fullStr |
A DNA‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators |
title_full_unstemmed |
A DNA‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators |
title_sort |
dna‐based method for identification of krill species and its application to analysing the diet of marine vertebrate predators |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1046/j.1365-294x.2002.01641.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294X.2002.01641.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294X.2002.01641.x |
genre |
Adelie penguin Balaenoptera musculus Blue whale Pygoscelis adeliae |
genre_facet |
Adelie penguin Balaenoptera musculus Blue whale Pygoscelis adeliae |
op_source |
Molecular Ecology volume 11, issue 12, page 2679-2690 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-294x.2002.01641.x |
container_title |
Molecular Ecology |
container_volume |
11 |
container_issue |
12 |
container_start_page |
2679 |
op_container_end_page |
2690 |
_version_ |
1800753019690680320 |