Genetic relationships in the peregrine falcon ( Falco peregrinus) analysed by microsatellite DNA markers
Abstract Microsatellite DNA markers were developed from a peregrine falcon ( Falco peregrinus ) and genetic relationships among peregrine falcons in southern Norway were analysed using the markers. The genomic DNA library was screened for the presence of dinucleotide microsatellite repeats. Twelve l...
Published in: | Molecular Ecology |
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Online Access: | http://dx.doi.org/10.1046/j.1365-294x.2000.00834.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294x.2000.00834.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294x.2000.00834.x |
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crwiley:10.1046/j.1365-294x.2000.00834.x 2024-06-02T08:06:20+00:00 Genetic relationships in the peregrine falcon ( Falco peregrinus) analysed by microsatellite DNA markers Nesje, M. Røed, K. H. Lifjeld, J. T. Lindberg, P. Steen, O. F. 2000 http://dx.doi.org/10.1046/j.1365-294x.2000.00834.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294x.2000.00834.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294x.2000.00834.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology volume 9, issue 1, page 53-60 ISSN 0962-1083 1365-294X journal-article 2000 crwiley https://doi.org/10.1046/j.1365-294x.2000.00834.x 2024-05-03T11:25:19Z Abstract Microsatellite DNA markers were developed from a peregrine falcon ( Falco peregrinus ) and genetic relationships among peregrine falcons in southern Norway were analysed using the markers. The genomic DNA library was screened for the presence of dinucleotide microsatellite repeats. Twelve loci revealed polymorphism through the initial analysis of 24 unrelated peregrine falcons, and Mendelian inheritance was confirmed in two peregrine falcon families bred in captivity. The estimated mean probability of identical genotypes in two unrelated individuals was 3 × 10 −8 , and the combined exclusion probability for parentage testing was 0.99 and 0.94 for one or both parents unknown, respectively. The markers were used to investigate the parentage of peregrine broods from the same nest site from different breeding seasons, and subsequently the nest‐site fidelity of the breeding peregrines. High nest‐site fidelity was found by studying pairwise comparisons of relatedness ( r xy ) estimates among chicks at six nest sites from three different breeding seasons. Cross‐species amplifications showed that most loci also appeared to amplify polymorphic products in the gyrfalcon ( F. rusticolus ), merlin ( F. columbarius ), hobby ( F. subbuteo ) and kestrel ( F. tinnunculus ), demonstrating that the loci will provide powerful genetic markers in these falcons too. Article in Journal/Newspaper Falco peregrinus gyrfalcon peregrine falcon Wiley Online Library Norway Molecular Ecology 9 1 53 60 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Microsatellite DNA markers were developed from a peregrine falcon ( Falco peregrinus ) and genetic relationships among peregrine falcons in southern Norway were analysed using the markers. The genomic DNA library was screened for the presence of dinucleotide microsatellite repeats. Twelve loci revealed polymorphism through the initial analysis of 24 unrelated peregrine falcons, and Mendelian inheritance was confirmed in two peregrine falcon families bred in captivity. The estimated mean probability of identical genotypes in two unrelated individuals was 3 × 10 −8 , and the combined exclusion probability for parentage testing was 0.99 and 0.94 for one or both parents unknown, respectively. The markers were used to investigate the parentage of peregrine broods from the same nest site from different breeding seasons, and subsequently the nest‐site fidelity of the breeding peregrines. High nest‐site fidelity was found by studying pairwise comparisons of relatedness ( r xy ) estimates among chicks at six nest sites from three different breeding seasons. Cross‐species amplifications showed that most loci also appeared to amplify polymorphic products in the gyrfalcon ( F. rusticolus ), merlin ( F. columbarius ), hobby ( F. subbuteo ) and kestrel ( F. tinnunculus ), demonstrating that the loci will provide powerful genetic markers in these falcons too. |
format |
Article in Journal/Newspaper |
author |
Nesje, M. Røed, K. H. Lifjeld, J. T. Lindberg, P. Steen, O. F. |
spellingShingle |
Nesje, M. Røed, K. H. Lifjeld, J. T. Lindberg, P. Steen, O. F. Genetic relationships in the peregrine falcon ( Falco peregrinus) analysed by microsatellite DNA markers |
author_facet |
Nesje, M. Røed, K. H. Lifjeld, J. T. Lindberg, P. Steen, O. F. |
author_sort |
Nesje, M. |
title |
Genetic relationships in the peregrine falcon ( Falco peregrinus) analysed by microsatellite DNA markers |
title_short |
Genetic relationships in the peregrine falcon ( Falco peregrinus) analysed by microsatellite DNA markers |
title_full |
Genetic relationships in the peregrine falcon ( Falco peregrinus) analysed by microsatellite DNA markers |
title_fullStr |
Genetic relationships in the peregrine falcon ( Falco peregrinus) analysed by microsatellite DNA markers |
title_full_unstemmed |
Genetic relationships in the peregrine falcon ( Falco peregrinus) analysed by microsatellite DNA markers |
title_sort |
genetic relationships in the peregrine falcon ( falco peregrinus) analysed by microsatellite dna markers |
publisher |
Wiley |
publishDate |
2000 |
url |
http://dx.doi.org/10.1046/j.1365-294x.2000.00834.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294x.2000.00834.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294x.2000.00834.x |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Falco peregrinus gyrfalcon peregrine falcon |
genre_facet |
Falco peregrinus gyrfalcon peregrine falcon |
op_source |
Molecular Ecology volume 9, issue 1, page 53-60 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-294x.2000.00834.x |
container_title |
Molecular Ecology |
container_volume |
9 |
container_issue |
1 |
container_start_page |
53 |
op_container_end_page |
60 |
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1800751265623310336 |