A suite of falcon single‐locus minisatellite probes: a powerful alternative to DNA fingerprinting
Nine falcon single‐locus minisatellite probes have been cloned, characterized and shown to provide a powerful alternative to multilocus DNA fingerprinting for determining the parentage of broods of young. Eight clones derived from size‐selected peregrine Falco peregrinus and merlin F. columbarius ch...
Published in: | Molecular Ecology |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1997
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1046/j.1365-294x.1997.00161.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294X.1997.00161.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294X.1997.00161.x |
id |
crwiley:10.1046/j.1365-294x.1997.00161.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1046/j.1365-294x.1997.00161.x 2024-09-30T14:34:37+00:00 A suite of falcon single‐locus minisatellite probes: a powerful alternative to DNA fingerprinting Wetton, J. H. Parkin, D. T. 1997 http://dx.doi.org/10.1046/j.1365-294x.1997.00161.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294X.1997.00161.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294X.1997.00161.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology volume 6, issue 2, page 119-128 ISSN 0962-1083 1365-294X journal-article 1997 crwiley https://doi.org/10.1046/j.1365-294x.1997.00161.x 2024-09-17T04:44:47Z Nine falcon single‐locus minisatellite probes have been cloned, characterized and shown to provide a powerful alternative to multilocus DNA fingerprinting for determining the parentage of broods of young. Eight clones derived from size‐selected peregrine Falco peregrinus and merlin F. columbarius charomid libraries were found to detect single minisatellite loci in all tested members of the genus Falco . A further clone (c Fti 1) randomly selected from a kestrel F. tinnunculus library identified a single locus in other members of the genus and certain species of the Accipitridae. The nine loci display a mean heterozygosity of 88% and considerable allelic diversity in the peregrine and merlin. Pedigree analysis provides evidence consistent with the clustering of minisatellites in linkage groups that are conserved between species. Mutations were observed at five loci among families of peregrines and kestrels. Composite profiles produced with this suite of probes provide an excellent means of confirming identity and parentage. The use of profiling for investigating familial relationships, population dynamics and combating the illegal trade in wild falcons is discussed. Article in Journal/Newspaper Falco peregrinus Wiley Online Library Molecular Ecology 6 2 119 128 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Nine falcon single‐locus minisatellite probes have been cloned, characterized and shown to provide a powerful alternative to multilocus DNA fingerprinting for determining the parentage of broods of young. Eight clones derived from size‐selected peregrine Falco peregrinus and merlin F. columbarius charomid libraries were found to detect single minisatellite loci in all tested members of the genus Falco . A further clone (c Fti 1) randomly selected from a kestrel F. tinnunculus library identified a single locus in other members of the genus and certain species of the Accipitridae. The nine loci display a mean heterozygosity of 88% and considerable allelic diversity in the peregrine and merlin. Pedigree analysis provides evidence consistent with the clustering of minisatellites in linkage groups that are conserved between species. Mutations were observed at five loci among families of peregrines and kestrels. Composite profiles produced with this suite of probes provide an excellent means of confirming identity and parentage. The use of profiling for investigating familial relationships, population dynamics and combating the illegal trade in wild falcons is discussed. |
format |
Article in Journal/Newspaper |
author |
Wetton, J. H. Parkin, D. T. |
spellingShingle |
Wetton, J. H. Parkin, D. T. A suite of falcon single‐locus minisatellite probes: a powerful alternative to DNA fingerprinting |
author_facet |
Wetton, J. H. Parkin, D. T. |
author_sort |
Wetton, J. H. |
title |
A suite of falcon single‐locus minisatellite probes: a powerful alternative to DNA fingerprinting |
title_short |
A suite of falcon single‐locus minisatellite probes: a powerful alternative to DNA fingerprinting |
title_full |
A suite of falcon single‐locus minisatellite probes: a powerful alternative to DNA fingerprinting |
title_fullStr |
A suite of falcon single‐locus minisatellite probes: a powerful alternative to DNA fingerprinting |
title_full_unstemmed |
A suite of falcon single‐locus minisatellite probes: a powerful alternative to DNA fingerprinting |
title_sort |
suite of falcon single‐locus minisatellite probes: a powerful alternative to dna fingerprinting |
publisher |
Wiley |
publishDate |
1997 |
url |
http://dx.doi.org/10.1046/j.1365-294x.1997.00161.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-294X.1997.00161.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-294X.1997.00161.x |
genre |
Falco peregrinus |
genre_facet |
Falco peregrinus |
op_source |
Molecular Ecology volume 6, issue 2, page 119-128 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-294x.1997.00161.x |
container_title |
Molecular Ecology |
container_volume |
6 |
container_issue |
2 |
container_start_page |
119 |
op_container_end_page |
128 |
_version_ |
1811638176269205504 |