Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.)
Abstract Atypical Aeromonas salmonicida (AAS) causes generalized lethal infections in farmed Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.), and is thus a serious threat for culture of these fish species. Virulence factors were studied among isolates of AAS fr...
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crwiley:10.1046/j.1365-2761.2003.00470.x 2024-06-02T08:00:02+00:00 Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.) Madetoja, J Pylkkö, P Pohjanvirta, T Schildt, L Pelkonen, S 2003 http://dx.doi.org/10.1046/j.1365-2761.2003.00470.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.2003.00470.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.2003.00470.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 26, issue 6, page 349-359 ISSN 0140-7775 1365-2761 journal-article 2003 crwiley https://doi.org/10.1046/j.1365-2761.2003.00470.x 2024-05-03T12:06:53Z Abstract Atypical Aeromonas salmonicida (AAS) causes generalized lethal infections in farmed Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.), and is thus a serious threat for culture of these fish species. Virulence factors were studied among isolates of AAS from Arctic charr ( n = 20), European grayling ( n = 19) and other fish species ( n = 20), of which 48 were of Finnish and 11 of Swedish origin. All isolates produced an A‐layer. Extracellular products (ECP) of the AAS isolates did not produce detectable gelatinase and caseinase activity in test assays. Analysis of the same ECP preparations with substrate sodium dodecyl sulphate–polyacrylamide gel electrophoresis showed weak proteolytic activity, indicating the different sensitivity of the detection methods used. The ECP from AAS isolates showed low cytotoxic activity against cultured cells. However, the ECP did not induce mortality in challenged Arctic charr. The results suggest that toxic components, like ECP, secreted by the bacterium may not be the major virulence factor in AAS‐infection in Arctic charr and European grayling, and hence the pathogenesis also differs from the pathogenesis of AAS‐infection in Atlantic salmon, Salmo salar L. Article in Journal/Newspaper Arctic charr Arctic Atlantic salmon Salmo salar Salvelinus alpinus Wiley Online Library Arctic Journal of Fish Diseases 26 6 349 359 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Atypical Aeromonas salmonicida (AAS) causes generalized lethal infections in farmed Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.), and is thus a serious threat for culture of these fish species. Virulence factors were studied among isolates of AAS from Arctic charr ( n = 20), European grayling ( n = 19) and other fish species ( n = 20), of which 48 were of Finnish and 11 of Swedish origin. All isolates produced an A‐layer. Extracellular products (ECP) of the AAS isolates did not produce detectable gelatinase and caseinase activity in test assays. Analysis of the same ECP preparations with substrate sodium dodecyl sulphate–polyacrylamide gel electrophoresis showed weak proteolytic activity, indicating the different sensitivity of the detection methods used. The ECP from AAS isolates showed low cytotoxic activity against cultured cells. However, the ECP did not induce mortality in challenged Arctic charr. The results suggest that toxic components, like ECP, secreted by the bacterium may not be the major virulence factor in AAS‐infection in Arctic charr and European grayling, and hence the pathogenesis also differs from the pathogenesis of AAS‐infection in Atlantic salmon, Salmo salar L. |
format |
Article in Journal/Newspaper |
author |
Madetoja, J Pylkkö, P Pohjanvirta, T Schildt, L Pelkonen, S |
spellingShingle |
Madetoja, J Pylkkö, P Pohjanvirta, T Schildt, L Pelkonen, S Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.) |
author_facet |
Madetoja, J Pylkkö, P Pohjanvirta, T Schildt, L Pelkonen, S |
author_sort |
Madetoja, J |
title |
Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.) |
title_short |
Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.) |
title_full |
Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.) |
title_fullStr |
Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.) |
title_full_unstemmed |
Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.) |
title_sort |
putative virulence factors of atypical aeromonas salmonicida isolated from arctic charr, salvelinus alpinus (l.), and european grayling, thymallus thymallus (l.) |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1046/j.1365-2761.2003.00470.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.2003.00470.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.2003.00470.x |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Atlantic salmon Salmo salar Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Atlantic salmon Salmo salar Salvelinus alpinus |
op_source |
Journal of Fish Diseases volume 26, issue 6, page 349-359 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-2761.2003.00470.x |
container_title |
Journal of Fish Diseases |
container_volume |
26 |
container_issue |
6 |
container_start_page |
349 |
op_container_end_page |
359 |
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1800744054386851840 |