Isolation of a highly pathogenic Vibrio pelagius strain associated with mass mortalities of turbot, Scophthalmus maximus (L.), larvae
Abstract A bacterial strain, characterized as Vibrio pelagius (Hq 222), was isolated from a turbot, Scophthalmus maximus (L.), larvae mass mortality in a commercial fish farm in Spain. Turbot larvae, post‐larvae (0.2 g) and juveniles (5 and 15 g) were experimentally infected. The bacterium appeared...
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crwiley:10.1046/j.1365-2761.2003.00461.x 2024-06-23T07:56:36+00:00 Isolation of a highly pathogenic Vibrio pelagius strain associated with mass mortalities of turbot, Scophthalmus maximus (L.), larvae Villamil, L Figueras, A Toranzo, A E Planas, M Novoa, B 2003 http://dx.doi.org/10.1046/j.1365-2761.2003.00461.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.2003.00461.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.2003.00461.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 26, issue 5, page 293-303 ISSN 0140-7775 1365-2761 journal-article 2003 crwiley https://doi.org/10.1046/j.1365-2761.2003.00461.x 2024-06-13T04:20:51Z Abstract A bacterial strain, characterized as Vibrio pelagius (Hq 222), was isolated from a turbot, Scophthalmus maximus (L.), larvae mass mortality in a commercial fish farm in Spain. Turbot larvae, post‐larvae (0.2 g) and juveniles (5 and 15 g) were experimentally infected. The bacterium appeared to be very virulent for larvae and post‐larvae, LD 50 being <5 bacteria mL −1 for larvae 1 week post‐infection and 3.9 × 10 5 bacteria mL −1 in post‐larvae at day 12 post‐infection. The bacterial strain was recovered in pure culture from the internal organs of infected fish. Histological lesions in post‐larvae exhibited swelling and necrosis of gill secondary lamellae, sloughing of intestinal mucosa and necrosis of haematopoietic tissue in the kidney. Vibrio pelagius (Hq 222) was able to grow in sterile sea water when incubated at room temperature or at 15 °C. Vibrio pelagius (Hq 222) was more adherent to the turbot cell lines TV‐1 and TF than Escherichia coli . In both cell lines, the number of adhered bacteria increased with incubation time. Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library Journal of Fish Diseases 26 5 293 303 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract A bacterial strain, characterized as Vibrio pelagius (Hq 222), was isolated from a turbot, Scophthalmus maximus (L.), larvae mass mortality in a commercial fish farm in Spain. Turbot larvae, post‐larvae (0.2 g) and juveniles (5 and 15 g) were experimentally infected. The bacterium appeared to be very virulent for larvae and post‐larvae, LD 50 being <5 bacteria mL −1 for larvae 1 week post‐infection and 3.9 × 10 5 bacteria mL −1 in post‐larvae at day 12 post‐infection. The bacterial strain was recovered in pure culture from the internal organs of infected fish. Histological lesions in post‐larvae exhibited swelling and necrosis of gill secondary lamellae, sloughing of intestinal mucosa and necrosis of haematopoietic tissue in the kidney. Vibrio pelagius (Hq 222) was able to grow in sterile sea water when incubated at room temperature or at 15 °C. Vibrio pelagius (Hq 222) was more adherent to the turbot cell lines TV‐1 and TF than Escherichia coli . In both cell lines, the number of adhered bacteria increased with incubation time. |
format |
Article in Journal/Newspaper |
author |
Villamil, L Figueras, A Toranzo, A E Planas, M Novoa, B |
spellingShingle |
Villamil, L Figueras, A Toranzo, A E Planas, M Novoa, B Isolation of a highly pathogenic Vibrio pelagius strain associated with mass mortalities of turbot, Scophthalmus maximus (L.), larvae |
author_facet |
Villamil, L Figueras, A Toranzo, A E Planas, M Novoa, B |
author_sort |
Villamil, L |
title |
Isolation of a highly pathogenic Vibrio pelagius strain associated with mass mortalities of turbot, Scophthalmus maximus (L.), larvae |
title_short |
Isolation of a highly pathogenic Vibrio pelagius strain associated with mass mortalities of turbot, Scophthalmus maximus (L.), larvae |
title_full |
Isolation of a highly pathogenic Vibrio pelagius strain associated with mass mortalities of turbot, Scophthalmus maximus (L.), larvae |
title_fullStr |
Isolation of a highly pathogenic Vibrio pelagius strain associated with mass mortalities of turbot, Scophthalmus maximus (L.), larvae |
title_full_unstemmed |
Isolation of a highly pathogenic Vibrio pelagius strain associated with mass mortalities of turbot, Scophthalmus maximus (L.), larvae |
title_sort |
isolation of a highly pathogenic vibrio pelagius strain associated with mass mortalities of turbot, scophthalmus maximus (l.), larvae |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1046/j.1365-2761.2003.00461.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.2003.00461.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.2003.00461.x |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Journal of Fish Diseases volume 26, issue 5, page 293-303 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-2761.2003.00461.x |
container_title |
Journal of Fish Diseases |
container_volume |
26 |
container_issue |
5 |
container_start_page |
293 |
op_container_end_page |
303 |
_version_ |
1802649860442161152 |