An alkaline phosphatase‐based method for the detection of infectious salmon anaemia virus (ISAV) in tissue culture and tissue imprints
Abstract Currently, the presence of infectious salmon anaemia virus (ISAV) is often detected in Atlantic salmon by the use of an indirect fluorescent antibody test. This test is limited by the poor stability of fluorescein isothiocyanate which fades after about a week in storage, preventing the deve...
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Online Access: | http://dx.doi.org/10.1046/j.1365-2761.2002.00408.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.2002.00408.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.2002.00408.x |
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crwiley:10.1046/j.1365-2761.2002.00408.x 2024-06-02T08:03:39+00:00 An alkaline phosphatase‐based method for the detection of infectious salmon anaemia virus (ISAV) in tissue culture and tissue imprints Wilson, L McBeath, S J Adamson, K L Cook, P F Ellis, L M Bricknell, I R 2002 http://dx.doi.org/10.1046/j.1365-2761.2002.00408.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.2002.00408.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.2002.00408.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 25, issue 10, page 615-619 ISSN 0140-7775 1365-2761 journal-article 2002 crwiley https://doi.org/10.1046/j.1365-2761.2002.00408.x 2024-05-03T10:50:38Z Abstract Currently, the presence of infectious salmon anaemia virus (ISAV) is often detected in Atlantic salmon by the use of an indirect fluorescent antibody test. This test is limited by the poor stability of fluorescein isothiocyanate which fades after about a week in storage, preventing the development of stained archive material as a reference source. One possible alternative would be the use of immunohistochemical staining methods to detect ISAV. An immunohistochemical method is presented that uses alkaline phosphatase‐conjugated antibodies and Vector ® Red as a substrate, to detect ISAV in kidney imprints. This paper also describes a procedure where Bouin's fluid is used to successfully inhibit endogenous alkaline phosphatase in tissue samples, prior to immunohistochemical processing. This method provides a stable stain that can be read for many weeks after staining or archived for future reference. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Journal of Fish Diseases 25 10 615 619 |
institution |
Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Currently, the presence of infectious salmon anaemia virus (ISAV) is often detected in Atlantic salmon by the use of an indirect fluorescent antibody test. This test is limited by the poor stability of fluorescein isothiocyanate which fades after about a week in storage, preventing the development of stained archive material as a reference source. One possible alternative would be the use of immunohistochemical staining methods to detect ISAV. An immunohistochemical method is presented that uses alkaline phosphatase‐conjugated antibodies and Vector ® Red as a substrate, to detect ISAV in kidney imprints. This paper also describes a procedure where Bouin's fluid is used to successfully inhibit endogenous alkaline phosphatase in tissue samples, prior to immunohistochemical processing. This method provides a stable stain that can be read for many weeks after staining or archived for future reference. |
format |
Article in Journal/Newspaper |
author |
Wilson, L McBeath, S J Adamson, K L Cook, P F Ellis, L M Bricknell, I R |
spellingShingle |
Wilson, L McBeath, S J Adamson, K L Cook, P F Ellis, L M Bricknell, I R An alkaline phosphatase‐based method for the detection of infectious salmon anaemia virus (ISAV) in tissue culture and tissue imprints |
author_facet |
Wilson, L McBeath, S J Adamson, K L Cook, P F Ellis, L M Bricknell, I R |
author_sort |
Wilson, L |
title |
An alkaline phosphatase‐based method for the detection of infectious salmon anaemia virus (ISAV) in tissue culture and tissue imprints |
title_short |
An alkaline phosphatase‐based method for the detection of infectious salmon anaemia virus (ISAV) in tissue culture and tissue imprints |
title_full |
An alkaline phosphatase‐based method for the detection of infectious salmon anaemia virus (ISAV) in tissue culture and tissue imprints |
title_fullStr |
An alkaline phosphatase‐based method for the detection of infectious salmon anaemia virus (ISAV) in tissue culture and tissue imprints |
title_full_unstemmed |
An alkaline phosphatase‐based method for the detection of infectious salmon anaemia virus (ISAV) in tissue culture and tissue imprints |
title_sort |
alkaline phosphatase‐based method for the detection of infectious salmon anaemia virus (isav) in tissue culture and tissue imprints |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1046/j.1365-2761.2002.00408.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.2002.00408.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.2002.00408.x |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Fish Diseases volume 25, issue 10, page 615-619 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-2761.2002.00408.x |
container_title |
Journal of Fish Diseases |
container_volume |
25 |
container_issue |
10 |
container_start_page |
615 |
op_container_end_page |
619 |
_version_ |
1800748228192239616 |