In situ adherence of Vibrio spp. to cryosections of Atlantic salmon, Salmo salar L., tissue.
Pathogenic and presumed non‐pathogenic bacteria isolated from fish were tested for their adhesion to cryosections from different mucosal surfaces of Atlantic salmon, Salmo salar L. Adhered bacteria were detected by immunohistochemistry. Mucus was stained and fixed with Alcian blue after incubation o...
Published in: | Journal of Fish Diseases |
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crwiley:10.1046/j.1365-2761.1999.00183.x 2024-10-06T13:47:21+00:00 In situ adherence of Vibrio spp. to cryosections of Atlantic salmon, Salmo salar L., tissue. Knudsen, G. Sørum, H. Press, C MC.L. Olafsen, J A. 1999 http://dx.doi.org/10.1046/j.1365-2761.1999.00183.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1999.00183.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1999.00183.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 22, issue 6, page 409-418 ISSN 0140-7775 1365-2761 journal-article 1999 crwiley https://doi.org/10.1046/j.1365-2761.1999.00183.x 2024-09-11T04:13:41Z Pathogenic and presumed non‐pathogenic bacteria isolated from fish were tested for their adhesion to cryosections from different mucosal surfaces of Atlantic salmon, Salmo salar L. Adhered bacteria were detected by immunohistochemistry. Mucus was stained and fixed with Alcian blue after incubation of bacteria. The majority of the bacteria tested, i.e. Vibrio anguillarum serotype O1 , Vibrio salmonicida , Vibrio viscosus, Flexibacter maritimus and ‘gut vibrios’, i.e. Vibrio iliopiscarius and intestinal isolates of V. salmonicida , all adhered to mucus on all salmon epithelial surfaces tested, including sections from the foregut, hindgut, pyloric caeca, gills and skin. In contrast, V. anguillarum serotype O2, including both serotypes O2a and O2b, did not adhere to mucus, but did adhere to all other components of the tissues. As a positive control for adhesion of bacteria on cryosections, Escherichia coli was bound to piglet ileal mucosal lining, and as a negative control for adhesion, Staphylococcus aureus was found not to bind to any of the tissues tested. The present study shows that adhesion to mucus was not restricted to pathogenic bacteria, and furthermore, that not all pathogenic bacteria studied adhered to mucus. Hence, on the basis of these findings, the present authors suggest that V. anguillarum O2 may have an invasion strategy which does not involve adhesion to mucus, and thus, differs from the other pathogenic bacteria in the present study, which all bound to salmon mucus. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 22 6 409 418 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Pathogenic and presumed non‐pathogenic bacteria isolated from fish were tested for their adhesion to cryosections from different mucosal surfaces of Atlantic salmon, Salmo salar L. Adhered bacteria were detected by immunohistochemistry. Mucus was stained and fixed with Alcian blue after incubation of bacteria. The majority of the bacteria tested, i.e. Vibrio anguillarum serotype O1 , Vibrio salmonicida , Vibrio viscosus, Flexibacter maritimus and ‘gut vibrios’, i.e. Vibrio iliopiscarius and intestinal isolates of V. salmonicida , all adhered to mucus on all salmon epithelial surfaces tested, including sections from the foregut, hindgut, pyloric caeca, gills and skin. In contrast, V. anguillarum serotype O2, including both serotypes O2a and O2b, did not adhere to mucus, but did adhere to all other components of the tissues. As a positive control for adhesion of bacteria on cryosections, Escherichia coli was bound to piglet ileal mucosal lining, and as a negative control for adhesion, Staphylococcus aureus was found not to bind to any of the tissues tested. The present study shows that adhesion to mucus was not restricted to pathogenic bacteria, and furthermore, that not all pathogenic bacteria studied adhered to mucus. Hence, on the basis of these findings, the present authors suggest that V. anguillarum O2 may have an invasion strategy which does not involve adhesion to mucus, and thus, differs from the other pathogenic bacteria in the present study, which all bound to salmon mucus. |
format |
Article in Journal/Newspaper |
author |
Knudsen, G. Sørum, H. Press, C MC.L. Olafsen, J A. |
spellingShingle |
Knudsen, G. Sørum, H. Press, C MC.L. Olafsen, J A. In situ adherence of Vibrio spp. to cryosections of Atlantic salmon, Salmo salar L., tissue. |
author_facet |
Knudsen, G. Sørum, H. Press, C MC.L. Olafsen, J A. |
author_sort |
Knudsen, G. |
title |
In situ adherence of Vibrio spp. to cryosections of Atlantic salmon, Salmo salar L., tissue. |
title_short |
In situ adherence of Vibrio spp. to cryosections of Atlantic salmon, Salmo salar L., tissue. |
title_full |
In situ adherence of Vibrio spp. to cryosections of Atlantic salmon, Salmo salar L., tissue. |
title_fullStr |
In situ adherence of Vibrio spp. to cryosections of Atlantic salmon, Salmo salar L., tissue. |
title_full_unstemmed |
In situ adherence of Vibrio spp. to cryosections of Atlantic salmon, Salmo salar L., tissue. |
title_sort |
in situ adherence of vibrio spp. to cryosections of atlantic salmon, salmo salar l., tissue. |
publisher |
Wiley |
publishDate |
1999 |
url |
http://dx.doi.org/10.1046/j.1365-2761.1999.00183.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1999.00183.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1999.00183.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 22, issue 6, page 409-418 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-2761.1999.00183.x |
container_title |
Journal of Fish Diseases |
container_volume |
22 |
container_issue |
6 |
container_start_page |
409 |
op_container_end_page |
418 |
_version_ |
1812175574018293760 |