Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L.
The field use of a staphylococcal coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples from Atlantic salmon, Salmo salar L., was evaluated. The COA test was compared with an immunohistochemical (IHC) method for the detection of clinical outbre...
Published in: | Journal of Fish Diseases |
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Format: | Article in Journal/Newspaper |
Language: | English |
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1999
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Online Access: | http://dx.doi.org/10.1046/j.1365-2761.1999.00154.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1999.00154.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1999.00154.x https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-2761.1999.00154.x |
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crwiley:10.1046/j.1365-2761.1999.00154.x 2024-06-02T08:03:30+00:00 Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L. Taksdal, T. Thorud, K. 1999 http://dx.doi.org/10.1046/j.1365-2761.1999.00154.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1999.00154.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1999.00154.x https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-2761.1999.00154.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 22, issue 2, page 117-124 ISSN 0140-7775 1365-2761 journal-article 1999 crwiley https://doi.org/10.1046/j.1365-2761.1999.00154.x 2024-05-03T10:43:24Z The field use of a staphylococcal coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples from Atlantic salmon, Salmo salar L., was evaluated. The COA test was compared with an immunohistochemical (IHC) method for the detection of clinical outbreaks of infectious pancreatic necrosis (IPN). The present paper describes the evaluation of 320 COA test results performed at local fish health laboratories in Norway from 1994 to 1996, and COA test results from two infection trials with IPNV. The agreement between the COA test and the IHC was very good. The agreement beyond chance, measured as kappa values, was 0.74 in individuals and 0.90 in pooled samples. Thus, the COA test was suited for the detection of outbreaks of IPN. Covert infections with IPNV remained undetected by the COA test. The minimum IPNV titre needed to obtain a positive COA test was ≈ 10 5 TCID 50 mL –1 . Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Norway Journal of Fish Diseases 22 2 117 124 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
The field use of a staphylococcal coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples from Atlantic salmon, Salmo salar L., was evaluated. The COA test was compared with an immunohistochemical (IHC) method for the detection of clinical outbreaks of infectious pancreatic necrosis (IPN). The present paper describes the evaluation of 320 COA test results performed at local fish health laboratories in Norway from 1994 to 1996, and COA test results from two infection trials with IPNV. The agreement between the COA test and the IHC was very good. The agreement beyond chance, measured as kappa values, was 0.74 in individuals and 0.90 in pooled samples. Thus, the COA test was suited for the detection of outbreaks of IPN. Covert infections with IPNV remained undetected by the COA test. The minimum IPNV titre needed to obtain a positive COA test was ≈ 10 5 TCID 50 mL –1 . |
format |
Article in Journal/Newspaper |
author |
Taksdal, T. Thorud, K. |
spellingShingle |
Taksdal, T. Thorud, K. Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L. |
author_facet |
Taksdal, T. Thorud, K. |
author_sort |
Taksdal, T. |
title |
Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L. |
title_short |
Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L. |
title_full |
Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L. |
title_fullStr |
Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L. |
title_full_unstemmed |
Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L. |
title_sort |
evaluation of a rapid coagglutination (coa) test for the detection of infectious pancreatic necrosis virus (ipnv) in tissue samples of atlantic salmon, salmo salar l. |
publisher |
Wiley |
publishDate |
1999 |
url |
http://dx.doi.org/10.1046/j.1365-2761.1999.00154.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1999.00154.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1999.00154.x https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-2761.1999.00154.x |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 22, issue 2, page 117-124 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-2761.1999.00154.x |
container_title |
Journal of Fish Diseases |
container_volume |
22 |
container_issue |
2 |
container_start_page |
117 |
op_container_end_page |
124 |
_version_ |
1800748047384182784 |