Isolation, cultivation and characterization of head kidney macrophages from Atlantic cod, Gadus morhua L.
Head kidney macrophages from Atlantic cod, Gadus morhua L., were isolated by density sedimentation and maintained under serum‐free conditions for up to one week. The cells adhered and spread well on glass and plastic, were highly phagocytic, and had typical macrophage morphology as shown by phase co...
Published in: | Journal of Fish Diseases |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1997
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1046/j.1365-2761.1997.d01-112.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1997.d01-112.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1997.d01-112.x |
id |
crwiley:10.1046/j.1365-2761.1997.d01-112.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1046/j.1365-2761.1997.d01-112.x 2024-06-02T08:03:08+00:00 Isolation, cultivation and characterization of head kidney macrophages from Atlantic cod, Gadus morhua L. Sørensen, K K Sveinbjørnsson, B Dalmo, R A Smedsrød, B Bertheussen, K 1997 http://dx.doi.org/10.1046/j.1365-2761.1997.d01-112.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1997.d01-112.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1997.d01-112.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 20, issue 2, page 93-107 ISSN 0140-7775 1365-2761 journal-article 1997 crwiley https://doi.org/10.1046/j.1365-2761.1997.d01-112.x 2024-05-03T11:48:26Z Head kidney macrophages from Atlantic cod, Gadus morhua L., were isolated by density sedimentation and maintained under serum‐free conditions for up to one week. The cells adhered and spread well on glass and plastic, were highly phagocytic, and had typical macrophage morphology as shown by phase contrast and scanning and transmission electron microscopy. Histochemical studies with the light microscope showed that the macrophages were acid phosphatase and non‐specific esterase positive, and alkaline phosphatase and peroxidase negative. Compared with unstimulated control cells, LPS‐treated cells showed enhanced superoxide anion formation, as measured by the reduction of nitroblue tetrazolium, and increased levels of acid phosphatase activity. Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library Journal of Fish Diseases 20 2 93 107 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Head kidney macrophages from Atlantic cod, Gadus morhua L., were isolated by density sedimentation and maintained under serum‐free conditions for up to one week. The cells adhered and spread well on glass and plastic, were highly phagocytic, and had typical macrophage morphology as shown by phase contrast and scanning and transmission electron microscopy. Histochemical studies with the light microscope showed that the macrophages were acid phosphatase and non‐specific esterase positive, and alkaline phosphatase and peroxidase negative. Compared with unstimulated control cells, LPS‐treated cells showed enhanced superoxide anion formation, as measured by the reduction of nitroblue tetrazolium, and increased levels of acid phosphatase activity. |
format |
Article in Journal/Newspaper |
author |
Sørensen, K K Sveinbjørnsson, B Dalmo, R A Smedsrød, B Bertheussen, K |
spellingShingle |
Sørensen, K K Sveinbjørnsson, B Dalmo, R A Smedsrød, B Bertheussen, K Isolation, cultivation and characterization of head kidney macrophages from Atlantic cod, Gadus morhua L. |
author_facet |
Sørensen, K K Sveinbjørnsson, B Dalmo, R A Smedsrød, B Bertheussen, K |
author_sort |
Sørensen, K K |
title |
Isolation, cultivation and characterization of head kidney macrophages from Atlantic cod, Gadus morhua L. |
title_short |
Isolation, cultivation and characterization of head kidney macrophages from Atlantic cod, Gadus morhua L. |
title_full |
Isolation, cultivation and characterization of head kidney macrophages from Atlantic cod, Gadus morhua L. |
title_fullStr |
Isolation, cultivation and characterization of head kidney macrophages from Atlantic cod, Gadus morhua L. |
title_full_unstemmed |
Isolation, cultivation and characterization of head kidney macrophages from Atlantic cod, Gadus morhua L. |
title_sort |
isolation, cultivation and characterization of head kidney macrophages from atlantic cod, gadus morhua l. |
publisher |
Wiley |
publishDate |
1997 |
url |
http://dx.doi.org/10.1046/j.1365-2761.1997.d01-112.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1997.d01-112.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1997.d01-112.x |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Journal of Fish Diseases volume 20, issue 2, page 93-107 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-2761.1997.d01-112.x |
container_title |
Journal of Fish Diseases |
container_volume |
20 |
container_issue |
2 |
container_start_page |
93 |
op_container_end_page |
107 |
_version_ |
1800747597437075456 |