Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus
Three cases of pyogranulomatous cephalic osteochrondritis of intensively reared rainbow trout, Oncorhynchus mykiss (Walbaum), and Atlantic salmon, Salmo salar L., in Canada and Chile, respectively, were examined for histopathological and bacteriological changes, and by using immunohistochemistry. Bi...
Published in: | Journal of Fish Diseases |
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crwiley:10.1046/j.1365-2761.1997.00323.x 2024-06-23T07:51:18+00:00 Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus Ostland, V.E. McGrogan, D.G. Ferguson, H.W. 1997 http://dx.doi.org/10.1046/j.1365-2761.1997.00323.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1997.00323.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1997.00323.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 20, issue 6, page 443-451 ISSN 0140-7775 1365-2761 journal-article 1997 crwiley https://doi.org/10.1046/j.1365-2761.1997.00323.x 2024-05-31T08:13:47Z Three cases of pyogranulomatous cephalic osteochrondritis of intensively reared rainbow trout, Oncorhynchus mykiss (Walbaum), and Atlantic salmon, Salmo salar L., in Canada and Chile, respectively, were examined for histopathological and bacteriological changes, and by using immunohistochemistry. Bilateral exophthalmia and intraocular haemorrhage were the most common gross lesions seen in the Ontario fish. Histologically, the major pathological changes seen in all cases involved the developing bone and cartilage of the head region, including the eye. Necrosis and pyogranulomatous inflammation of the scleral cartilage (necrotic scleritis) were the major ocular changes. Similarly, lysis of cephalic cartilage and immature bone, accompanied by pyogranulomatous inflammation, usually progressing to fibrosis, were the most common lesions associated with the cranial changes in both the rainbow trout and Atlantic salmon. Routine histological staining, including special stains, failed to reveal the presence of pathogens associated with these lesions. However, immunohistochemical staining of representative sections from all cases with rabbit anti‐ Flexibacter psychrophilus serum (ATCC 49510) demonstrated significant numbers of antigenically related bacteria within the leading edge of some of the scleral and cephalic osteochondritic lesions. Axenic cultures of Flexibacter psychrophilus were recovered from the eye lesions of the rainbow trout, but it was not possible to reproduce ocular lesions experimentally. However, mortality was produced following intramuscular injection of rainbow trout with bacteria. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Canada Journal of Fish Diseases 20 6 443 451 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Three cases of pyogranulomatous cephalic osteochrondritis of intensively reared rainbow trout, Oncorhynchus mykiss (Walbaum), and Atlantic salmon, Salmo salar L., in Canada and Chile, respectively, were examined for histopathological and bacteriological changes, and by using immunohistochemistry. Bilateral exophthalmia and intraocular haemorrhage were the most common gross lesions seen in the Ontario fish. Histologically, the major pathological changes seen in all cases involved the developing bone and cartilage of the head region, including the eye. Necrosis and pyogranulomatous inflammation of the scleral cartilage (necrotic scleritis) were the major ocular changes. Similarly, lysis of cephalic cartilage and immature bone, accompanied by pyogranulomatous inflammation, usually progressing to fibrosis, were the most common lesions associated with the cranial changes in both the rainbow trout and Atlantic salmon. Routine histological staining, including special stains, failed to reveal the presence of pathogens associated with these lesions. However, immunohistochemical staining of representative sections from all cases with rabbit anti‐ Flexibacter psychrophilus serum (ATCC 49510) demonstrated significant numbers of antigenically related bacteria within the leading edge of some of the scleral and cephalic osteochondritic lesions. Axenic cultures of Flexibacter psychrophilus were recovered from the eye lesions of the rainbow trout, but it was not possible to reproduce ocular lesions experimentally. However, mortality was produced following intramuscular injection of rainbow trout with bacteria. |
format |
Article in Journal/Newspaper |
author |
Ostland, V.E. McGrogan, D.G. Ferguson, H.W. |
spellingShingle |
Ostland, V.E. McGrogan, D.G. Ferguson, H.W. Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus |
author_facet |
Ostland, V.E. McGrogan, D.G. Ferguson, H.W. |
author_sort |
Ostland, V.E. |
title |
Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus |
title_short |
Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus |
title_full |
Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus |
title_fullStr |
Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus |
title_full_unstemmed |
Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus |
title_sort |
cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with flexibacter psychrophilus |
publisher |
Wiley |
publishDate |
1997 |
url |
http://dx.doi.org/10.1046/j.1365-2761.1997.00323.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2761.1997.00323.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2761.1997.00323.x |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 20, issue 6, page 443-451 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1365-2761.1997.00323.x |
container_title |
Journal of Fish Diseases |
container_volume |
20 |
container_issue |
6 |
container_start_page |
443 |
op_container_end_page |
451 |
_version_ |
1802642356449574912 |