The two myostatin genes of Atlantic salmon ( Salmo salar) are expressed in a variety of tissues
Two myostatin isoforms were identified in Atlantic salmon ( Salmo salar ) by RT‐PCR, and genomic sequences encoding this negative muscle growth factor were for the first time isolated from a nonmammalian species. Salmon myostatin isoform I is transcribed in white skeletal muscle as a 2346‐nucleotide...
Published in: | European Journal of Biochemistry |
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Online Access: | http://dx.doi.org/10.1046/j.0014-2956.2001.02456.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.0014-2956.2001.02456.x http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.0014-2956.2001.02456.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.0014-2956.2001.02456.x |
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crwiley:10.1046/j.0014-2956.2001.02456.x 2024-06-02T08:03:36+00:00 The two myostatin genes of Atlantic salmon ( Salmo salar) are expressed in a variety of tissues Østbye, Tone‐Kari Galloway, Trina Falck Nielsen, Christer Gabestad, Irene Bardal, Tora Andersen, Øivind 2001 http://dx.doi.org/10.1046/j.0014-2956.2001.02456.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.0014-2956.2001.02456.x http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.0014-2956.2001.02456.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.0014-2956.2001.02456.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor European Journal of Biochemistry volume 268, issue 20, page 5249-5257 ISSN 0014-2956 1432-1033 journal-article 2001 crwiley https://doi.org/10.1046/j.0014-2956.2001.02456.x 2024-05-03T11:26:48Z Two myostatin isoforms were identified in Atlantic salmon ( Salmo salar ) by RT‐PCR, and genomic sequences encoding this negative muscle growth factor were for the first time isolated from a nonmammalian species. Salmon myostatin isoform I is transcribed in white skeletal muscle as a 2346‐nucleotide mRNA species that encodes a precursor protein of 373 amino acids. Salmon myostatin I shows 93% sequence identity with isoform II which was isolated from white muscle as a partial cDNA sequence of 1409 nucleotides. In contrast to the restricted gene expression of myostatin in mammals, salmon myostatin I and II mRNAs were identified by RT‐PCR in multiple tissues, including white muscle, intestine, brain, gills, tongue and eye. In addition, isoform I mRNA was found in red skeletal muscle, heart, spleen, and ovarian tissue. Using polyclonal antibodies against both isoforms, a 55‐kDa precursor protein was detected by Western blot analysis in the red and white skeletal muscle, heart, intestine, and brain. Immunoreactive peptides of 35–40 kDa were identified in the gills, tongue, spleen, and head kidney, while the 25‐kDa mature myostatin was found in the eye and serum, and in vitro expressed in rabbit reticulocyte lysate. Salmon myostatin was immunohistochemically localized in the sarcoplasma of red and white muscle fibres, in intestinal epithelial cells, at the basis of the branchial primary lamellae, and in odontoblasts and ameloblasts of the tongue teeth. The results indicate that the role of fish myostatin may not be restricted to muscle growth regulation, but may have additional functions similar to the growth/differentiation factor‐11 in mammals. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library European Journal of Biochemistry 268 20 5249 5257 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Two myostatin isoforms were identified in Atlantic salmon ( Salmo salar ) by RT‐PCR, and genomic sequences encoding this negative muscle growth factor were for the first time isolated from a nonmammalian species. Salmon myostatin isoform I is transcribed in white skeletal muscle as a 2346‐nucleotide mRNA species that encodes a precursor protein of 373 amino acids. Salmon myostatin I shows 93% sequence identity with isoform II which was isolated from white muscle as a partial cDNA sequence of 1409 nucleotides. In contrast to the restricted gene expression of myostatin in mammals, salmon myostatin I and II mRNAs were identified by RT‐PCR in multiple tissues, including white muscle, intestine, brain, gills, tongue and eye. In addition, isoform I mRNA was found in red skeletal muscle, heart, spleen, and ovarian tissue. Using polyclonal antibodies against both isoforms, a 55‐kDa precursor protein was detected by Western blot analysis in the red and white skeletal muscle, heart, intestine, and brain. Immunoreactive peptides of 35–40 kDa were identified in the gills, tongue, spleen, and head kidney, while the 25‐kDa mature myostatin was found in the eye and serum, and in vitro expressed in rabbit reticulocyte lysate. Salmon myostatin was immunohistochemically localized in the sarcoplasma of red and white muscle fibres, in intestinal epithelial cells, at the basis of the branchial primary lamellae, and in odontoblasts and ameloblasts of the tongue teeth. The results indicate that the role of fish myostatin may not be restricted to muscle growth regulation, but may have additional functions similar to the growth/differentiation factor‐11 in mammals. |
format |
Article in Journal/Newspaper |
author |
Østbye, Tone‐Kari Galloway, Trina Falck Nielsen, Christer Gabestad, Irene Bardal, Tora Andersen, Øivind |
spellingShingle |
Østbye, Tone‐Kari Galloway, Trina Falck Nielsen, Christer Gabestad, Irene Bardal, Tora Andersen, Øivind The two myostatin genes of Atlantic salmon ( Salmo salar) are expressed in a variety of tissues |
author_facet |
Østbye, Tone‐Kari Galloway, Trina Falck Nielsen, Christer Gabestad, Irene Bardal, Tora Andersen, Øivind |
author_sort |
Østbye, Tone‐Kari |
title |
The two myostatin genes of Atlantic salmon ( Salmo salar) are expressed in a variety of tissues |
title_short |
The two myostatin genes of Atlantic salmon ( Salmo salar) are expressed in a variety of tissues |
title_full |
The two myostatin genes of Atlantic salmon ( Salmo salar) are expressed in a variety of tissues |
title_fullStr |
The two myostatin genes of Atlantic salmon ( Salmo salar) are expressed in a variety of tissues |
title_full_unstemmed |
The two myostatin genes of Atlantic salmon ( Salmo salar) are expressed in a variety of tissues |
title_sort |
two myostatin genes of atlantic salmon ( salmo salar) are expressed in a variety of tissues |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1046/j.0014-2956.2001.02456.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.0014-2956.2001.02456.x http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.0014-2956.2001.02456.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.0014-2956.2001.02456.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
European Journal of Biochemistry volume 268, issue 20, page 5249-5257 ISSN 0014-2956 1432-1033 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.0014-2956.2001.02456.x |
container_title |
European Journal of Biochemistry |
container_volume |
268 |
container_issue |
20 |
container_start_page |
5249 |
op_container_end_page |
5257 |
_version_ |
1800748173146193920 |