Effects of hypo‐osmotic stress on ATP release in isolated turbot ( Scophthalmus maximus) hepatocytes
Background information . ATP is released from many cell types exposed to hypo‐osmotic shock and is involved in RVD (regulatory volume decrease). Purinergic signalling events have been extensively investigated in mammals, but not in marine teleosteans. Results . The effect of hypo‐osmotic shock on AT...
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crwiley:10.1042/bc20050077 2024-09-09T20:06:40+00:00 Effects of hypo‐osmotic stress on ATP release in isolated turbot ( Scophthalmus maximus) hepatocytes Ollivier, Hélène Pichavant‐Rafini, Karine Puill‐Stephan, Eneour Calvès, Patrick Nonnotte, Liliane Nonnotte, Guy 2006 http://dx.doi.org/10.1042/bc20050077 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1042%2FBC20050077 https://onlinelibrary.wiley.com/doi/pdf/10.1042/BC20050077 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biology of the Cell volume 98, issue 7, page 427-437 ISSN 0248-4900 1768-322X journal-article 2006 crwiley https://doi.org/10.1042/bc20050077 2024-06-20T04:23:53Z Background information . ATP is released from many cell types exposed to hypo‐osmotic shock and is involved in RVD (regulatory volume decrease). Purinergic signalling events have been extensively investigated in mammals, but not in marine teleosteans. Results . The effect of hypo‐osmotic shock on ATP release was examined in isolated hepatocytes from turbot ( Scophthalmus maximus ), a marine flatfish. Hypo‐osmotic stress (240 mOsm·kg −1 ) induced a significant increase in ATP efflux, and was inhibited by a potential CFTR (cystic fibrosis transmembrane conductance regulator) inhibitor, glibenclamide, but not by the MDR1 (multidrug resistance 1) P‐glycoprotein inhibitor, verapamil. ATP efflux could be a cAMP‐dependent process, as IBMX (isobutylmethylxanthine) and forskolin triggered the process under iso‐osmotic conditions. Protein kinases, including protein kinase C, could also be involved, as staurosporine and chelerythrine inhibited the mechanism. Calcium could contribute to ATP efflux as ionomycin, a calcium ionophore, elicited a rapid release under iso‐osmotic conditions, and chelation using EGTA abolished ATP release under hypo‐osmotic conditions. RVD was partially abolished by apyrase, an ATP scavenger, and suramin, a purinoceptor antagonist. Moreover, hypo‐osmotic shock induced a rise in intracellular calcium which could be involved in RVD. Since extracellular ATP triggered an increase in cellular free‐calcium content under iso‐osmotic conditions, our results could indicate that hypo‐osmotic‐induced ATP efflux contributes to RVD in turbot hepatocytes by stimulating purinergic receptors, which may lead to activation of a calcium signalling pathway. Conclusions . These data provide the first evidence of volume‐sensitive ATP signalling for volume maintenance in a marine teleost fish cell type. Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library Biology of the Cell 98 7 427 437 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Background information . ATP is released from many cell types exposed to hypo‐osmotic shock and is involved in RVD (regulatory volume decrease). Purinergic signalling events have been extensively investigated in mammals, but not in marine teleosteans. Results . The effect of hypo‐osmotic shock on ATP release was examined in isolated hepatocytes from turbot ( Scophthalmus maximus ), a marine flatfish. Hypo‐osmotic stress (240 mOsm·kg −1 ) induced a significant increase in ATP efflux, and was inhibited by a potential CFTR (cystic fibrosis transmembrane conductance regulator) inhibitor, glibenclamide, but not by the MDR1 (multidrug resistance 1) P‐glycoprotein inhibitor, verapamil. ATP efflux could be a cAMP‐dependent process, as IBMX (isobutylmethylxanthine) and forskolin triggered the process under iso‐osmotic conditions. Protein kinases, including protein kinase C, could also be involved, as staurosporine and chelerythrine inhibited the mechanism. Calcium could contribute to ATP efflux as ionomycin, a calcium ionophore, elicited a rapid release under iso‐osmotic conditions, and chelation using EGTA abolished ATP release under hypo‐osmotic conditions. RVD was partially abolished by apyrase, an ATP scavenger, and suramin, a purinoceptor antagonist. Moreover, hypo‐osmotic shock induced a rise in intracellular calcium which could be involved in RVD. Since extracellular ATP triggered an increase in cellular free‐calcium content under iso‐osmotic conditions, our results could indicate that hypo‐osmotic‐induced ATP efflux contributes to RVD in turbot hepatocytes by stimulating purinergic receptors, which may lead to activation of a calcium signalling pathway. Conclusions . These data provide the first evidence of volume‐sensitive ATP signalling for volume maintenance in a marine teleost fish cell type. |
format |
Article in Journal/Newspaper |
author |
Ollivier, Hélène Pichavant‐Rafini, Karine Puill‐Stephan, Eneour Calvès, Patrick Nonnotte, Liliane Nonnotte, Guy |
spellingShingle |
Ollivier, Hélène Pichavant‐Rafini, Karine Puill‐Stephan, Eneour Calvès, Patrick Nonnotte, Liliane Nonnotte, Guy Effects of hypo‐osmotic stress on ATP release in isolated turbot ( Scophthalmus maximus) hepatocytes |
author_facet |
Ollivier, Hélène Pichavant‐Rafini, Karine Puill‐Stephan, Eneour Calvès, Patrick Nonnotte, Liliane Nonnotte, Guy |
author_sort |
Ollivier, Hélène |
title |
Effects of hypo‐osmotic stress on ATP release in isolated turbot ( Scophthalmus maximus) hepatocytes |
title_short |
Effects of hypo‐osmotic stress on ATP release in isolated turbot ( Scophthalmus maximus) hepatocytes |
title_full |
Effects of hypo‐osmotic stress on ATP release in isolated turbot ( Scophthalmus maximus) hepatocytes |
title_fullStr |
Effects of hypo‐osmotic stress on ATP release in isolated turbot ( Scophthalmus maximus) hepatocytes |
title_full_unstemmed |
Effects of hypo‐osmotic stress on ATP release in isolated turbot ( Scophthalmus maximus) hepatocytes |
title_sort |
effects of hypo‐osmotic stress on atp release in isolated turbot ( scophthalmus maximus) hepatocytes |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1042/bc20050077 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1042%2FBC20050077 https://onlinelibrary.wiley.com/doi/pdf/10.1042/BC20050077 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Biology of the Cell volume 98, issue 7, page 427-437 ISSN 0248-4900 1768-322X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1042/bc20050077 |
container_title |
Biology of the Cell |
container_volume |
98 |
container_issue |
7 |
container_start_page |
427 |
op_container_end_page |
437 |
_version_ |
1809939177845817344 |