Cloning and expression of three aquaporin homologues from the European eel ( Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression
Background information . The European eel ( Anguilla anguilla ) is able to osmoregulate over a wide range of environmental salinities from FW (freshwater) to hyperconcentrated SW (seawater). Successful acclimation is associated with strict regulation of ion and water transport pathways within key os...
Published in: | Biology of the Cell |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2005
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1042/bc20040111 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1042%2FBC20040111 https://onlinelibrary.wiley.com/doi/pdf/10.1042/BC20040111 |
id |
crwiley:10.1042/bc20040111 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1042/bc20040111 2024-09-15T17:39:38+00:00 Cloning and expression of three aquaporin homologues from the European eel ( Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression Martinez, Anne‐Sophie Cutler, Christopher P. Wilson, Gillian D. Phillips, Claire Hazon, Neil Cramb, Gordon 2005 http://dx.doi.org/10.1042/bc20040111 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1042%2FBC20040111 https://onlinelibrary.wiley.com/doi/pdf/10.1042/BC20040111 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biology of the Cell volume 97, issue 8, page 615-627 ISSN 0248-4900 1768-322X journal-article 2005 crwiley https://doi.org/10.1042/bc20040111 2024-08-30T04:11:35Z Background information . The European eel ( Anguilla anguilla ) is able to osmoregulate over a wide range of environmental salinities from FW (freshwater) to hyperconcentrated SW (seawater). Successful acclimation is associated with strict regulation of ion and water transport pathways within key osmoregulatory epithelia to enable animals to survive the dehydrating or oedematous conditions. These observations suggested that homologues of the AQP (aquaporin) water channel family were expressed in the eel and that these proteins may contribute to the water transport and osmoregulation in all euryhaline teleosts. Results . Complementary DNAs encoding a homologue of the mammalian aquaglyceroporins (termed AQPe) and two homologues of mammalian aquaporin‐1 [termed AQP1 and AQP1dup (aquaporin‐1 duplicate)] were isolated from the European eel. Northern‐blot analysis revealed (i) two AQP1 transcripts exhibiting a wide tissue distribution, (ii) a single AQP1dup mRNA transcript found in the kidney and the oesophagus, and (iii) a single AQPe mRNA detectable mainly in the kidney and the intestine. The relative expression of isoforms within the kidney was AQP1dup>AQPe>AQP1. SW acclimation significantly reduced the abundance of AQP1, AQP1dup and AQPe transcripts in the kidney of yellow eels by approx. 72, 66 and 34$ respectively, whereas the expression levels in silver eels were independent of salinity and equivalent to those observed in yellow SW‐acclimated fish. AQP1 protein expression was primarily located within the vascular endothelium in yellow eels and the epithelial apical brush border in some renal tubules in silver eels. Infusion of cortisol into FW eels had no effect on AQPe mRNA expression, but induced significant decreases in AQP1 and AQP1dup mRNA levels in the kidney of yellow eels. Cortisol infusion had no effect on the expression of any isoform in the silver eels. Conclusions . These results suggest that SW‐acclimation or cortisol infusion induces a down‐regulation of renal AQP expression in yellow eels. ... Article in Journal/Newspaper Anguilla anguilla Wiley Online Library Biology of the Cell 97 8 615 627 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Background information . The European eel ( Anguilla anguilla ) is able to osmoregulate over a wide range of environmental salinities from FW (freshwater) to hyperconcentrated SW (seawater). Successful acclimation is associated with strict regulation of ion and water transport pathways within key osmoregulatory epithelia to enable animals to survive the dehydrating or oedematous conditions. These observations suggested that homologues of the AQP (aquaporin) water channel family were expressed in the eel and that these proteins may contribute to the water transport and osmoregulation in all euryhaline teleosts. Results . Complementary DNAs encoding a homologue of the mammalian aquaglyceroporins (termed AQPe) and two homologues of mammalian aquaporin‐1 [termed AQP1 and AQP1dup (aquaporin‐1 duplicate)] were isolated from the European eel. Northern‐blot analysis revealed (i) two AQP1 transcripts exhibiting a wide tissue distribution, (ii) a single AQP1dup mRNA transcript found in the kidney and the oesophagus, and (iii) a single AQPe mRNA detectable mainly in the kidney and the intestine. The relative expression of isoforms within the kidney was AQP1dup>AQPe>AQP1. SW acclimation significantly reduced the abundance of AQP1, AQP1dup and AQPe transcripts in the kidney of yellow eels by approx. 72, 66 and 34$ respectively, whereas the expression levels in silver eels were independent of salinity and equivalent to those observed in yellow SW‐acclimated fish. AQP1 protein expression was primarily located within the vascular endothelium in yellow eels and the epithelial apical brush border in some renal tubules in silver eels. Infusion of cortisol into FW eels had no effect on AQPe mRNA expression, but induced significant decreases in AQP1 and AQP1dup mRNA levels in the kidney of yellow eels. Cortisol infusion had no effect on the expression of any isoform in the silver eels. Conclusions . These results suggest that SW‐acclimation or cortisol infusion induces a down‐regulation of renal AQP expression in yellow eels. ... |
format |
Article in Journal/Newspaper |
author |
Martinez, Anne‐Sophie Cutler, Christopher P. Wilson, Gillian D. Phillips, Claire Hazon, Neil Cramb, Gordon |
spellingShingle |
Martinez, Anne‐Sophie Cutler, Christopher P. Wilson, Gillian D. Phillips, Claire Hazon, Neil Cramb, Gordon Cloning and expression of three aquaporin homologues from the European eel ( Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression |
author_facet |
Martinez, Anne‐Sophie Cutler, Christopher P. Wilson, Gillian D. Phillips, Claire Hazon, Neil Cramb, Gordon |
author_sort |
Martinez, Anne‐Sophie |
title |
Cloning and expression of three aquaporin homologues from the European eel ( Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression |
title_short |
Cloning and expression of three aquaporin homologues from the European eel ( Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression |
title_full |
Cloning and expression of three aquaporin homologues from the European eel ( Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression |
title_fullStr |
Cloning and expression of three aquaporin homologues from the European eel ( Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression |
title_full_unstemmed |
Cloning and expression of three aquaporin homologues from the European eel ( Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression |
title_sort |
cloning and expression of three aquaporin homologues from the european eel ( anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1042/bc20040111 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1042%2FBC20040111 https://onlinelibrary.wiley.com/doi/pdf/10.1042/BC20040111 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Biology of the Cell volume 97, issue 8, page 615-627 ISSN 0248-4900 1768-322X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1042/bc20040111 |
container_title |
Biology of the Cell |
container_volume |
97 |
container_issue |
8 |
container_start_page |
615 |
op_container_end_page |
627 |
_version_ |
1810481368361074688 |