Experimental optimization of enzymic kinetic resolution of racemic flurbiprofen
Immobilized lipase from Candida antarctica (Novozym® 435) was employed for the kinetic resolution of racemic flurbiprofen by the method of enantioselective esterification with alcohols. However, the presence of accumulated water from the esterification influenced the enantiomeric ratio and reaction...
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crwiley:10.1042/ba20040163 2024-09-15T17:46:18+00:00 Experimental optimization of enzymic kinetic resolution of racemic flurbiprofen Zhang, Hua Yun Wang, Xin Ching, Chi Bun Wu, Jin Chuan 2005 http://dx.doi.org/10.1042/ba20040163 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1042%2FBA20040163 https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1042/BA20040163 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology and Applied Biochemistry volume 42, issue 1, page 67-71 ISSN 0885-4513 1470-8744 journal-article 2005 crwiley https://doi.org/10.1042/ba20040163 2024-07-11T04:36:16Z Immobilized lipase from Candida antarctica (Novozym® 435) was employed for the kinetic resolution of racemic flurbiprofen by the method of enantioselective esterification with alcohols. However, the presence of accumulated water from the esterification influenced the enantiomeric ratio and reaction rate, due to increased rate of hydrolysis of the esterified enantiomer. In the present study, the procedure for optimizing the experimental resolution of the racemate was tested, with a focus on solvent and alcohol types, inhibition of alcohol substrates and the nature of the reversible reaction. The optimal concentration of feed flurbiprofen (180 mM or 44 mg/ml) was determined on basis of the maximum water content favourable for esterification, in single resolution, with the use of methanol in the solvent of cyclohexane. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Biotechnology and Applied Biochemistry 42 1 67 71 |
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Wiley Online Library |
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English |
description |
Immobilized lipase from Candida antarctica (Novozym® 435) was employed for the kinetic resolution of racemic flurbiprofen by the method of enantioselective esterification with alcohols. However, the presence of accumulated water from the esterification influenced the enantiomeric ratio and reaction rate, due to increased rate of hydrolysis of the esterified enantiomer. In the present study, the procedure for optimizing the experimental resolution of the racemate was tested, with a focus on solvent and alcohol types, inhibition of alcohol substrates and the nature of the reversible reaction. The optimal concentration of feed flurbiprofen (180 mM or 44 mg/ml) was determined on basis of the maximum water content favourable for esterification, in single resolution, with the use of methanol in the solvent of cyclohexane. |
format |
Article in Journal/Newspaper |
author |
Zhang, Hua Yun Wang, Xin Ching, Chi Bun Wu, Jin Chuan |
spellingShingle |
Zhang, Hua Yun Wang, Xin Ching, Chi Bun Wu, Jin Chuan Experimental optimization of enzymic kinetic resolution of racemic flurbiprofen |
author_facet |
Zhang, Hua Yun Wang, Xin Ching, Chi Bun Wu, Jin Chuan |
author_sort |
Zhang, Hua Yun |
title |
Experimental optimization of enzymic kinetic resolution of racemic flurbiprofen |
title_short |
Experimental optimization of enzymic kinetic resolution of racemic flurbiprofen |
title_full |
Experimental optimization of enzymic kinetic resolution of racemic flurbiprofen |
title_fullStr |
Experimental optimization of enzymic kinetic resolution of racemic flurbiprofen |
title_full_unstemmed |
Experimental optimization of enzymic kinetic resolution of racemic flurbiprofen |
title_sort |
experimental optimization of enzymic kinetic resolution of racemic flurbiprofen |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1042/ba20040163 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1042%2FBA20040163 https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1042/BA20040163 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Applied Biochemistry volume 42, issue 1, page 67-71 ISSN 0885-4513 1470-8744 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1042/ba20040163 |
container_title |
Biotechnology and Applied Biochemistry |
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42 |
container_issue |
1 |
container_start_page |
67 |
op_container_end_page |
71 |
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1810494315234852864 |