Investigations of Reaction Kinetics for Immobilized Enzymes—Identification of Parameters in the Presence of Diffusion Limitation
Abstract A method is proposed for identification of kinetic parameters when diffusion of substrates is limiting in reactions catalyzed by immobilized enzymes. This method overcomes conventional sequential procedures, which assume immobilization does not affect the conformation of the enzyme and, thu...
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crwiley:10.1021/bp060062e 2024-09-15T17:40:52+00:00 Investigations of Reaction Kinetics for Immobilized Enzymes—Identification of Parameters in the Presence of Diffusion Limitation Berendsen, Wouter R. Lapin, Alexei Reuss, Matthias 2006 http://dx.doi.org/10.1021/bp060062e http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1021%2Fbp060062e https://onlinelibrary.wiley.com/doi/full/10.1021/bp060062e en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology Progress volume 22, issue 5, page 1305-1312 ISSN 8756-7938 1520-6033 journal-article 2006 crwiley https://doi.org/10.1021/bp060062e 2024-08-13T04:18:57Z Abstract A method is proposed for identification of kinetic parameters when diffusion of substrates is limiting in reactions catalyzed by immobilized enzymes. This method overcomes conventional sequential procedures, which assume immobilization does not affect the conformation of the enzyme and, thus, consider intrinsic and inherent kinetics to be the same. The coupled equations describing intraparticle mass transport are solved simultaneously using numerical methods and are used for direct estimation of kinetic parameters by fitting modeling results to time‐course measurements in a stirred tank reactor. While most traditional procedures were based on Michaelis‐Menten kinetics, the method presented here is applicable to more complex kinetic mechanisms involving multiple state variables, such as ping‐pong bi‐bi. The method is applied to the kinetic resolution of ( R / S )‐1‐methoxy‐2‐propanol with vinyl acetate catalyzed by Candida antarctica lipase B. A mathematical model is developed consisting of irreversible ping‐pong bi‐bi kinetics, including competitive inhibition of both enantiomers. The kinetic model, which fits to experimental data over a wide range of both substrates (5–95%) and temperatures (5–56 °C), is used for simulations to study typical behavior of immobilized enzyme systems. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Biotechnology Progress 22 5 1305 1312 |
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Abstract A method is proposed for identification of kinetic parameters when diffusion of substrates is limiting in reactions catalyzed by immobilized enzymes. This method overcomes conventional sequential procedures, which assume immobilization does not affect the conformation of the enzyme and, thus, consider intrinsic and inherent kinetics to be the same. The coupled equations describing intraparticle mass transport are solved simultaneously using numerical methods and are used for direct estimation of kinetic parameters by fitting modeling results to time‐course measurements in a stirred tank reactor. While most traditional procedures were based on Michaelis‐Menten kinetics, the method presented here is applicable to more complex kinetic mechanisms involving multiple state variables, such as ping‐pong bi‐bi. The method is applied to the kinetic resolution of ( R / S )‐1‐methoxy‐2‐propanol with vinyl acetate catalyzed by Candida antarctica lipase B. A mathematical model is developed consisting of irreversible ping‐pong bi‐bi kinetics, including competitive inhibition of both enantiomers. The kinetic model, which fits to experimental data over a wide range of both substrates (5–95%) and temperatures (5–56 °C), is used for simulations to study typical behavior of immobilized enzyme systems. |
format |
Article in Journal/Newspaper |
author |
Berendsen, Wouter R. Lapin, Alexei Reuss, Matthias |
spellingShingle |
Berendsen, Wouter R. Lapin, Alexei Reuss, Matthias Investigations of Reaction Kinetics for Immobilized Enzymes—Identification of Parameters in the Presence of Diffusion Limitation |
author_facet |
Berendsen, Wouter R. Lapin, Alexei Reuss, Matthias |
author_sort |
Berendsen, Wouter R. |
title |
Investigations of Reaction Kinetics for Immobilized Enzymes—Identification of Parameters in the Presence of Diffusion Limitation |
title_short |
Investigations of Reaction Kinetics for Immobilized Enzymes—Identification of Parameters in the Presence of Diffusion Limitation |
title_full |
Investigations of Reaction Kinetics for Immobilized Enzymes—Identification of Parameters in the Presence of Diffusion Limitation |
title_fullStr |
Investigations of Reaction Kinetics for Immobilized Enzymes—Identification of Parameters in the Presence of Diffusion Limitation |
title_full_unstemmed |
Investigations of Reaction Kinetics for Immobilized Enzymes—Identification of Parameters in the Presence of Diffusion Limitation |
title_sort |
investigations of reaction kinetics for immobilized enzymes—identification of parameters in the presence of diffusion limitation |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1021/bp060062e http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1021%2Fbp060062e https://onlinelibrary.wiley.com/doi/full/10.1021/bp060062e |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology Progress volume 22, issue 5, page 1305-1312 ISSN 8756-7938 1520-6033 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1021/bp060062e |
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Biotechnology Progress |
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22 |
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5 |
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1305 |
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1312 |
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1810486947509960704 |