Role of dihydropyridine‐sensitive calcium channels in meiosis and fertilization in the bivalve molluscs Ruditapes philippinarum and Crassostrea gigas
Prophase‐arrested oocytes of Ruditapes philippinarum can not be fertilized or stimulated by a depolarizing agent such as an excess of KCl, in contrast to the situation found in Crassostrea gigas . We have performed a comparative study between the two situations found in these species. In vitro, both...
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crwiley:10.1016/s0248-4900(00)01069-8 2024-06-02T08:05:36+00:00 Role of dihydropyridine‐sensitive calcium channels in meiosis and fertilization in the bivalve molluscs Ruditapes philippinarum and Crassostrea gigas Leclerc, Catherine Guerrier, Pierre Moreau, Marc 2000 http://dx.doi.org/10.1016/s0248-4900(00)01069-8 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0248-4900(00)01069-8 https://onlinelibrary.wiley.com/doi/pdf/10.1016/S0248-4900%2800%2901069-8 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biology of the Cell volume 92, issue 3-4, page 285-299 ISSN 0248-4900 1768-322X journal-article 2000 crwiley https://doi.org/10.1016/s0248-4900(00)01069-8 2024-05-03T11:53:23Z Prophase‐arrested oocytes of Ruditapes philippinarum can not be fertilized or stimulated by a depolarizing agent such as an excess of KCl, in contrast to the situation found in Crassostrea gigas . We have performed a comparative study between the two situations found in these species. In vitro, both of these oocytes can be triggered to reinitiate meiosis following a treatment by serotonin which promotes an intracellular calcium surge. Ruditapes and Crassostrea oocytes further arrest in metaphase I, at which stage they can be either activated by sperm or by excess KCl. These treatments trigger an intracellular calcium increase. This suggests that functional voltage‐operated Ca 2+ channels are expressed in Ruditapes during the course of maturation between prophase and metaphase I. Results obtained using pharmacological tools and direct binding of specific dihydropyridines, strongly suggest that these channels are dihydropyridine‐sensitive calcium channels. In Ruditapes they become functional after 5‐HT stimulation, their number increasing before GVBD. In Crassostrea the dihydropyridine‐sensitive Ca 2+ channels are already present at prophase stage and their density is constant from prophase to metaphase I. Moreover, we have shown for Ruditapes and Crassostrea that: 1) the addition of 10 μM of S(‐)BayK8644, an agonist of dihydropyridine‐sensitive calcium channels to metaphase‐arrested oocytes releases them from metaphase block; and 2) incubating these oocytes with nicardipine, a potent blocker of dihydropyridine‐sensitive Ca 2+ channels, inhibits both their activation by excess KCl or fertilization. Taken together these data suggest that the absence of dihydropyridine‐sensitive Ca 2+ channels in the membrane of prophase‐arrested oocytes of Ruditapes may account for their inability to be fertilized at this stage, while the presence of dihydropyridine‐sensitive Ca 2+ channels in prophase‐arrested oocytes of Crassostrea may explain their fertilizability at this stage. Article in Journal/Newspaper Crassostrea gigas Wiley Online Library Biology of the Cell 92 3-4 285 299 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Prophase‐arrested oocytes of Ruditapes philippinarum can not be fertilized or stimulated by a depolarizing agent such as an excess of KCl, in contrast to the situation found in Crassostrea gigas . We have performed a comparative study between the two situations found in these species. In vitro, both of these oocytes can be triggered to reinitiate meiosis following a treatment by serotonin which promotes an intracellular calcium surge. Ruditapes and Crassostrea oocytes further arrest in metaphase I, at which stage they can be either activated by sperm or by excess KCl. These treatments trigger an intracellular calcium increase. This suggests that functional voltage‐operated Ca 2+ channels are expressed in Ruditapes during the course of maturation between prophase and metaphase I. Results obtained using pharmacological tools and direct binding of specific dihydropyridines, strongly suggest that these channels are dihydropyridine‐sensitive calcium channels. In Ruditapes they become functional after 5‐HT stimulation, their number increasing before GVBD. In Crassostrea the dihydropyridine‐sensitive Ca 2+ channels are already present at prophase stage and their density is constant from prophase to metaphase I. Moreover, we have shown for Ruditapes and Crassostrea that: 1) the addition of 10 μM of S(‐)BayK8644, an agonist of dihydropyridine‐sensitive calcium channels to metaphase‐arrested oocytes releases them from metaphase block; and 2) incubating these oocytes with nicardipine, a potent blocker of dihydropyridine‐sensitive Ca 2+ channels, inhibits both their activation by excess KCl or fertilization. Taken together these data suggest that the absence of dihydropyridine‐sensitive Ca 2+ channels in the membrane of prophase‐arrested oocytes of Ruditapes may account for their inability to be fertilized at this stage, while the presence of dihydropyridine‐sensitive Ca 2+ channels in prophase‐arrested oocytes of Crassostrea may explain their fertilizability at this stage. |
format |
Article in Journal/Newspaper |
author |
Leclerc, Catherine Guerrier, Pierre Moreau, Marc |
spellingShingle |
Leclerc, Catherine Guerrier, Pierre Moreau, Marc Role of dihydropyridine‐sensitive calcium channels in meiosis and fertilization in the bivalve molluscs Ruditapes philippinarum and Crassostrea gigas |
author_facet |
Leclerc, Catherine Guerrier, Pierre Moreau, Marc |
author_sort |
Leclerc, Catherine |
title |
Role of dihydropyridine‐sensitive calcium channels in meiosis and fertilization in the bivalve molluscs Ruditapes philippinarum and Crassostrea gigas |
title_short |
Role of dihydropyridine‐sensitive calcium channels in meiosis and fertilization in the bivalve molluscs Ruditapes philippinarum and Crassostrea gigas |
title_full |
Role of dihydropyridine‐sensitive calcium channels in meiosis and fertilization in the bivalve molluscs Ruditapes philippinarum and Crassostrea gigas |
title_fullStr |
Role of dihydropyridine‐sensitive calcium channels in meiosis and fertilization in the bivalve molluscs Ruditapes philippinarum and Crassostrea gigas |
title_full_unstemmed |
Role of dihydropyridine‐sensitive calcium channels in meiosis and fertilization in the bivalve molluscs Ruditapes philippinarum and Crassostrea gigas |
title_sort |
role of dihydropyridine‐sensitive calcium channels in meiosis and fertilization in the bivalve molluscs ruditapes philippinarum and crassostrea gigas |
publisher |
Wiley |
publishDate |
2000 |
url |
http://dx.doi.org/10.1016/s0248-4900(00)01069-8 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0248-4900(00)01069-8 https://onlinelibrary.wiley.com/doi/pdf/10.1016/S0248-4900%2800%2901069-8 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Biology of the Cell volume 92, issue 3-4, page 285-299 ISSN 0248-4900 1768-322X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1016/s0248-4900(00)01069-8 |
container_title |
Biology of the Cell |
container_volume |
92 |
container_issue |
3-4 |
container_start_page |
285 |
op_container_end_page |
299 |
_version_ |
1800750468623761408 |