A RNA polymerase with transcriptional activity at 0°C from the Antarctic bacterium Pseudomonas syringae
A DNA‐dependent RNA polymerase was purified from the Antarctic psychrotrophic bacterium Pseudomonas syringae . The RNA polymerase showed a typical eubacterial subunit composition with β, β′, α 2 and σ subunits. The subunits cross‐reacted with antibodies raised against holoenzyme and the individual s...
Published in: | FEBS Letters |
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Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1999
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Subjects: | |
Online Access: | http://dx.doi.org/10.1016/s0014-5793(99)00660-2 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2899%2900660-2 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(99)00660-2 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2899%2900660-2 |
Summary: | A DNA‐dependent RNA polymerase was purified from the Antarctic psychrotrophic bacterium Pseudomonas syringae . The RNA polymerase showed a typical eubacterial subunit composition with β, β′, α 2 and σ subunits. The subunits cross‐reacted with antibodies raised against holoenzyme and the individual subunits of the RNA polymerase of Escherichia coli . However, the enzyme was considered unique, since unlike the RNA polymerase of mesophilic E. coli it exhibited significant and consistent transcriptional activity (10–15%) even at 0°C. But, similar to the enzyme from the mesophilic bacterium, the RNA polymerase from P. syringae exhibited optimum activity at 37°C. The study also demonstrates that the RNA polymerase of P. syringae could preferentially transcribe the cold‐inducible gene cspA of E. coli only at lower temperatures (0–22°C). The polymerase was also observed to be relatively more rifampicin‐resistant during transcription at lower temperature. |
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