Cholinesterases from the common oyster ( Crassostrea gigas)
Marine bivalves such as oysters and mussels are widely used as bioindicators of contamination in the monitoring of pollutant effects. As filter feeders, these species are known to be good general indicators of chemical contamination. However, the efficient use of decreased acetylcholinesterase activ...
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Online Access: | http://dx.doi.org/10.1016/s0014-5793(97)00339-6 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2897%2900339-6 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(97)00339-6 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2897%2900339-6 |
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crwiley:10.1016/s0014-5793(97)00339-6 2024-09-15T18:03:08+00:00 Cholinesterases from the common oyster ( Crassostrea gigas) Evidence for the presence of a soluble acetylcholinesterase insensitive to organophosphate and carbamate inhibitors Bocquené, Gilles Roig, Anne Fournier, Didier 1997 http://dx.doi.org/10.1016/s0014-5793(97)00339-6 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2897%2900339-6 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(97)00339-6 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2897%2900339-6 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor FEBS Letters volume 407, issue 3, page 261-266 ISSN 0014-5793 1873-3468 journal-article 1997 crwiley https://doi.org/10.1016/s0014-5793(97)00339-6 2024-08-27T04:29:26Z Marine bivalves such as oysters and mussels are widely used as bioindicators of contamination in the monitoring of pollutant effects. As filter feeders, these species are known to be good general indicators of chemical contamination. However, the efficient use of decreased acetylcholinesterase activity in the oyster as a biomarker of exposure to neurotoxic compounds requires a definition of the different types of cholinesterases coexisting in this mollusk. This study reports the partial purification, separation and characterization of two cholinesterases extracted from the oyster Crassostrea gigas . Differences in apparent molecular weight, type of glycosylation and hydrophobicity, and sensitivity to inhibitors suggest that they are encoded by two different genes. ‘A’ cholinesterase (apparent molecular weight 200 kDa) is anchored to the membrane via a glycolipid, is not glycosylated but sensitive to organophosphate and carbamate inhibitors. ‘B’ cholinesterase (molecular weight 330 kDa) is hydrophilic, glycosylated and highly resistant to organophosphate and carbamate inhibitors. The kinetic properties of these two cholinesterases were compared with those of other invertebrate cholinesterases. The presence of a cholinesterase insensitive to insecticides suggests that a significant improvement in the use of oyster cholinesterases as biomarkers of pollutant effects could be achieved by simple separation of the two forms. Article in Journal/Newspaper Crassostrea gigas Wiley Online Library FEBS Letters 407 3 261 266 |
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English |
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Marine bivalves such as oysters and mussels are widely used as bioindicators of contamination in the monitoring of pollutant effects. As filter feeders, these species are known to be good general indicators of chemical contamination. However, the efficient use of decreased acetylcholinesterase activity in the oyster as a biomarker of exposure to neurotoxic compounds requires a definition of the different types of cholinesterases coexisting in this mollusk. This study reports the partial purification, separation and characterization of two cholinesterases extracted from the oyster Crassostrea gigas . Differences in apparent molecular weight, type of glycosylation and hydrophobicity, and sensitivity to inhibitors suggest that they are encoded by two different genes. ‘A’ cholinesterase (apparent molecular weight 200 kDa) is anchored to the membrane via a glycolipid, is not glycosylated but sensitive to organophosphate and carbamate inhibitors. ‘B’ cholinesterase (molecular weight 330 kDa) is hydrophilic, glycosylated and highly resistant to organophosphate and carbamate inhibitors. The kinetic properties of these two cholinesterases were compared with those of other invertebrate cholinesterases. The presence of a cholinesterase insensitive to insecticides suggests that a significant improvement in the use of oyster cholinesterases as biomarkers of pollutant effects could be achieved by simple separation of the two forms. |
format |
Article in Journal/Newspaper |
author |
Bocquené, Gilles Roig, Anne Fournier, Didier |
spellingShingle |
Bocquené, Gilles Roig, Anne Fournier, Didier Cholinesterases from the common oyster ( Crassostrea gigas) |
author_facet |
Bocquené, Gilles Roig, Anne Fournier, Didier |
author_sort |
Bocquené, Gilles |
title |
Cholinesterases from the common oyster ( Crassostrea gigas) |
title_short |
Cholinesterases from the common oyster ( Crassostrea gigas) |
title_full |
Cholinesterases from the common oyster ( Crassostrea gigas) |
title_fullStr |
Cholinesterases from the common oyster ( Crassostrea gigas) |
title_full_unstemmed |
Cholinesterases from the common oyster ( Crassostrea gigas) |
title_sort |
cholinesterases from the common oyster ( crassostrea gigas) |
publisher |
Wiley |
publishDate |
1997 |
url |
http://dx.doi.org/10.1016/s0014-5793(97)00339-6 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2897%2900339-6 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(97)00339-6 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2897%2900339-6 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
FEBS Letters volume 407, issue 3, page 261-266 ISSN 0014-5793 1873-3468 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1016/s0014-5793(97)00339-6 |
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FEBS Letters |
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407 |
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3 |
container_start_page |
261 |
op_container_end_page |
266 |
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1810440647084081152 |